Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,365,526 IS2 (–) +5 bp coding (1244‑1248/1302 nt) dcuA ← C4‑dicarboxylate transporter DcuA

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)27 (0.930) 25/488 0.2 86.9% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 4365526 = 4 (0.140)coding (1248/1302 nt) dcuA C4‑dicarboxylate transporter DcuA
* ? NC_000913 = 3187426NA (NA)24 (0.830) 21/486 0.4 85.5% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 4365530 4 (0.140)coding (1244/1302 nt) dcuA C4‑dicarboxylate transporter DcuA

AACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGC  >  NC_000913/3185849‑3186340
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     
aacaaTACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAg                                                                                                                                                                                                                                                     <  2:164219/250‑1 (MQ=255)
          aCAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCt                                                                                                                                                                                                                                          >  1:184068/1‑251 (MQ=255)
              cTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAAt                                                                                                                                                                                                                                      >  1:97704/1‑251 (MQ=255)
                    ggAACTGAATTTCAAGGCTCGTATGGAATCCACAAGCAAAGATGACACACCGGGCAACGTTTACGCGCAGGCTGACTACAAGATTGCTTACGAGTAATAGAATTTGGCAGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGGTCAGCTGTCAAAAAAATTAAAATTAACCACAATATGCGAATTGATTAAAGAGATTTATTGTGCATTAACCGTTATTAGACAGACCCCCTTAATTTcccg                                                                                                                                                                                                                                >  2:336018/1‑249 (MQ=255)
                     gAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGa                                                                                                                                                                                                                               <  1:264147/251‑1 (MQ=255)
                               tCAACGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAatat                                                                                                                                                                                                                     >  1:36414/1‑251 (MQ=255)
                                        gTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAAt                                                                                                                                                                                                            >  1:253564/1‑251 (MQ=255)
                                                    cAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATAGCATTTGGCCGGGGTCGCCCTGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAACCTCCAGACAACCAATATCACGTAAATAAGTGATAGTCt                                                                                                                                                                                                <  2:97704/251‑1 (MQ=255)
                                                       gCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTaa                                                                                                                                                                                             <  1:278869/251‑1 (MQ=255)
                                                                     ccGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGttttt                                                                                                                                                                                  >  1:325555/1‑248 (MQ=255)
                                                                           aaCGTTTATGCTCAGGCTGACGCTAAGATTGCTTACGAGTAATTGCAGTTGTCTGGGGTCGCCCGTCAACATTATTAACGGATTAATGAATAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCTAATTTATTAAAAATATTTATTGTGCATTATCAGTTATTAGACTGGCCCCCTTAATCGCCAGACAACCAATATCACTTAAATAATTGATAGTGTTAATACTAGTTTTtagtctagtc                                                                                                                                                                         <  2:35035/251‑1 (MQ=255)
                                                                              gTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAtt                                                                                                                                                                      <  1:382122/251‑1 (MQ=255)
                                                                                                                        cATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGc                                                                                                                               >  1:160483/1‑248 (MQ=255)
                                                                                                                                                          ggATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTc                                                                                          <  1:353205/251‑1 (MQ=255)
                                                                                                                                                                   aTAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCt                                                                                >  1:273830/1‑251 (MQ=255)
                                                                                                                                                                   aTAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTAATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCCATATCACTTAAATAAGTGATAGTCTTAATACTCGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGACTTAGGGCCGGAGAAACGCAGTCGGCGCACCACACAGGCAAAGATCGGAATGGTTCAACCGA‑GCt                                                                                >  2:134405/1‑251 (MQ=21)
                                                                                                                                                                                       aaaaaTTAAAATTCACGACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTAATAAAATGGCCTCCTGAATCTCAAGAAAACTTTTATCACTTATATAAGTGATAGTCTTAATAATAGTTTTTAGACTAGTCATTGAAGAACAAATGATTGATGTATTAGGACCGGAGAAACGCAGACGGCGTACTACACAGGAACAGTTCGCAATTGTTC‑GCAAAAGATTTGAAACGGGGAGGACggac                                                            >  2:279077/1‑249 (MQ=255)
                                                                                                                                                                                                    aaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTACAGACAACCAATATCAATTAAATAAGTGATAGGCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGTTGTCTTAGGGCCGGAGAAACGCAGACGGCGCACCCCAAAGGCAAAGATAGCAAt                                                                                               >  2:384832/1‑204 (MQ=18)
                                                                                                                                                                                                    aaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTAGCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAt                                                                                               <  1:384832/204‑1 (MQ=255)
                                                                                                                                                                                                           aTAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAAc                                         >  1:379740/1‑250 (MQ=255)
                                                                                                                                                                                                           aTAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAAc                                         >  1:143830/1‑250 (MQ=255)
                                                                                                                                                                                                                cGAATTTATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGgtg                                   <  2:325555/251‑1 (MQ=255)
                                                                                                                                                                                                                 gAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGgtgt                                  >  1:380967/1‑251 (MQ=255)
                                                                                                                                                                                                                   aTTGATTAAAAATATTTAGTGTTCATTATCCGGTATTCGACTGGCCCCCTGAATCTCCAGACCACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCATCGTTGCCCGGCAACATGgtgt                                  >  1:104815/1‑249 (MQ=255)
                                                                                                                                                                                                                                       gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAgccagcca                      <  1:281052/241‑1 (MQ=37)
                                                                                                                                                                                                                                       gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGACTTAATACTAGTTTTTAGACTAGTCCTTGGAGAACAGATGATTGCTGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGACAAGATCGCAATTGTTCAGCAGC‑GCTTTGAACCGGGGATGGAGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGcagccagcaa                      >  2:281052/1‑239 (MQ=11)
                                                                                                                                                                                                                                             tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGt      >  1:255862/1‑251 (MQ=33)
                                                                                                                                                                                                                                                  gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGc  >  1:347358/1‑250 (MQ=31)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     
AACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGA‑GCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGC  >  NC_000913/3185849‑3186340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.