Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	820,914	IS5 (+) +4 bp	coding (122‑125/714 nt)	ybhM →	Bax1‑I family protein YbhM

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	273955 =	NA (NA)	22 (0.750)	20/488	0.4	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	820914 =	0 (0.000)					coding (122/714 nt)	ybhM	Bax1‑I family protein YbhM
*	?	NC_000913	= 275149	NA (NA)	26 (0.890)	25/486	0.2	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 820917	0 (0.000)					coding (125/714 nt)	ybhM	Bax1‑I family protein YbhM

AAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC‑AGTTGGTTATCGTTTTTCAGCAAC  >  NC_000913/273717‑274197                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    aaaGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGCAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAAt                                                                                                                                                                                                                                         >  1:294757/1‑251 (MQ=255)        ccAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCggg                                                                                                                                                                                                                                  >  1:369922/1‑251 (MQ=255)          aaaGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGa                                                                                                                                                                                                                                <  1:159316/251‑1 (MQ=255)           aaGCACTGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCGCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTGCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGaa                                                                                                                                                                                                                               <  2:136658/251‑1 (MQ=255)                      gAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGTAATTCTTCTCgg                                                                                                                                                                                                                    >  1:303141/1‑251 (MQ=255)                                                                       tCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGAGCCGATTTTTTCTCCCg                                                                                                                                                                   >  1:205173/1‑251 (MQ=255)                                                                          ggCTGAACATGGATAAGACTAAAAGCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaa                                                                                                                                                                <  1:81075/251‑1 (MQ=255)                                                                                  aTGGATAAGACTAAAAACCCCCATGTAAATGACGGATTTATATTTCTGGGACACAGGCACATTCGCAAACGCAGTCGTTATTGCGAGATGCGAGAGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCCCCGCATCGCTGACAGCACTGTTTTGGAAGGTGAGAATTAGAGGGGAAATTCTTCTAGGCTGACTACGTCTTTTCATTTCTTAATGTTTGAGACGATTTTTTTACCCGTaaaatcattg                                                                                                                                                        >  2:306815/1‑244 (MQ=255)                                                                                    ggATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGAAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACGTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                                                                                                                      <  1:195295/251‑1 (MQ=255)                                                                                                             aaTGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                                                                                                                              <  1:75341/250‑1 (MQ=255)                                                                                                                  cGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACTCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGCGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAtt                                                                                                                        >  1:53479/1‑251 (MQ=255)                                                                                                                  cGGCTTTATCTTTCAGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAtt                                                                                                                        >  1:383268/1‑251 (MQ=255)                                                                                                                               cTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc                                                                                                           >  1:22694/1‑251 (MQ=255)                                                                                                                                                    aaaCGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTATTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGACTTGAATCAGCCTATTTAGACAGTTTCTTCGCCATTTAAGGCGTTATCCCCCGTTTTTAGTGAGctctct                                                                                      >  1:902/1‑251 (MQ=255)                                                                                                                                                                        gaagCGAGAGGTCTCCACGATCCCGCAGGAGGAAGCCAGAAACTTCGCCGCATCGCTGAAAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTAAGTCATTTCATTTCTTCATGTTTGAGACGATTTTTTCTCACGTAAATACTTTGAATCAGCCTATTTAGACCGTTTCTTCGCAATTTAAGGTGTTTTTCACCTTTTTTAGTGAGATTTCTTCCACTTACGTTTCATTTgt                                                                  >  2:142723/4‑250 (MQ=17)                                                                                                                                                                                                 cAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCg                                         >  1:103130/1‑251 (MQ=255)                                                                                                                                                                                                       aaaGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATa                                   <  1:334019/251‑1 (MQ=255)                                                                                                                                                                                                               aaaCTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGcc                           <  1:12060/251‑1 (MQ=255)                                                                                                                                                                                                                      gcagatgcGCTGACAGCACTGTTATGGAAGGTGCGAATAAGAGGGGAAATTCTTCTCGGCTGACGCAGTCATTTCATTTTTTCATGGTGGAGCCGATTGTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCTTTTCTTCGCCATGTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGAC‑AGTTg                     <  2:15606/242‑1 (MQ=11)                                                                                                                                                                                                                       ccgcATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGCGGAAATTCTTCTCGGCTGACTCAATCATTTCATTTCTTCCTGTTTGAGACGATTTTTTCTACCGTAAATGCCTTGAATCAGCATATTTAGAACGTTTCTTCGCCATTTAAGGAGTTATACACAGTTTTTAGAGCGATCTCACACCCTGACGTCTCCTTTGGTCCGCCCGAAACAGGGTGGACAGCGTGAATAACATCGCC‑AGTTGGtt                  >  2:246793/1‑251 (MQ=255)                                                                                                                                                                                                                          cATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC‑AGTTGGTTATc               <  1:78963/251‑1 (MQ=38)                                                                                                                                                                                                                             cGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCTATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC‑AGTTGGTTATCGtt            <  1:113446/251‑1 (MQ=25)                                                                                                                                                                                                                                tGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC‑AGTTGGTTATCGttttt         >  1:142980/1‑251 (MQ=34)                                                                                                                                                                                                                                  aCAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCACAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCCGCATATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGCCGTATCATTTGGTCCGCCCGAACCCGGTTGGCAAGCGGGAATTACACCGCCCAGT‑GGTTATCGTTTTTc        >  2:288085/1‑250 (MQ=11)                                                                                                                                                                                                                                       aCTGTTATGTAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGCATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGAGTGAATAACATCGCC‑AGTTGGTTATCGTTTTTCAGCAAc  >  2:164619/1‑251 (MQ=14)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    AAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC‑AGTTGGTTATCGTTTTTCAGCAAC  >  NC_000913/273717‑274197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.