Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,207,5490G.92.7% 145.2 / 3.4 41coding (179/606 nt)ttdBL(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base . (21/17);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGT‑GCTGGCGGCAACCCAGGTGGAAGAGATTGAAG  >  NC_000913/3207317‑3207788
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
gctgGTTCATGCCGATCTCACCTTTGAAAATCTGCCTCACCCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATA‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGTTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaaaa                                                                                                                                                                                                                                <  1:337519/250‑1 (MQ=255)
       caggCCGATCTCCCCTTTGAAAATCTTGCTCCCACCCGGAGCGCGTTAGGAAAAAGAGCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCGCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGAc                                                                                                                                                                                                                         <  1:344222/247‑1 (MQ=255)
       cATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGCTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATACCTTACGATCTCCACGGCACAGCGCTTTTCCACGC‑GGCCCCATCGTGCGCAACAACGGCGAc                                                                                                                                                                                                                         >  2:266378/1‑250 (MQ=255)
            cGATCTCACCTTTGAACATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATACTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTAGAGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTTCGCAAAAACGACGCCAAATgg                                                                                                                                                                                                                   >  2:19427/1‑251 (MQ=255)
                ctcACCTTTGAAAATCTGTCTCACACCAGGAGCGCGCTCTGAAAAAGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGAAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGTCCTTCCGCGACGTTTGTCAACGCCGTTTGATCGACCTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGCTTTTCCACGCTG‑CCCCATCCGGCGCTAAAACGGCGACACATggg                                                                                                                                                                                                                  >  1:115018/1‑248 (MQ=255)
                        agaaaacatgtcgcccacacggcgcgcGTTAGGAAAAAGAACCTGACACCCCCGAAC‑AATGCTTAAGAGCGTCAAGATATGCGCGTCGTCGATGTTATCTACCTGGCCGTTACGCTGGTGACCGGCCGCGACGTTTGTCTCCGCCGTGTGATCGAACTGAAACTTCCGAGCCCTTACGATCTCAACGGCAAAGCGATTTTCTACGCTG‑CGCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTcc                                                                                                                                                                                                       <  1:222620/228‑1 (MQ=255)
                         aaaaattttactcACACCAGGATCGAGTTATGAAAAAAACCCTTAAAACCCGTACC‑AAAGCGAAAGTTCTGCAAGAGATCCGCGCCGGCGAGGTGATCTACCTGGCGGGTACGCTGGTGACCTGCCGCGACGTTTGTCCCCGCCGTGTGATCGACCTGAAACGTCCGAGCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCg                                                                                                                                                                                                      <  2:2468/241‑1 (MQ=255)
                             atcgttctcCCACCCGGAGCGCTTTATGAAAAATACCCTTACAACCCC‑ATCCAAAGCGGAAGATCTGCAAGATATTCGTGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCTCTACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCgg                                                                                                                                                                                                  <  1:370992/246‑1 (MQ=255)
                                tGTCTCACAACCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGTTA‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTTACCGGTACGCTGGTGACCTGACGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCAGATACCTTACGTTCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATAGTGCGCAACAACGGCGCCAAATGGAAGATGGTCACCGTCGGccc                                                                                                                                                                                               >  2:157853/1‑251 (MQ=255)
                                               gcgcGTTATGAAAAAGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCg                                                                                                                                                                                <  1:16892/251‑1 (MQ=255)
                                                      aTGAAAAAGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACACCCAGTATGCGTATGGa                                                                                                                                                                          >  1:210909/1‑250 (MQ=255)
                                                        gAAAAAGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGAGCAAAAACGGCGACAAATGGGAGCTGTTCTCCGTCGGCCCGACAACCAGTATGCGTATGGaa                                                                                                                                                                         >  1:57570/1‑249 (MQ=255)
                                                        aaaaaaGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGGCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAg                                                                                                                                                                       <  1:276053/250‑1 (MQ=255)
                                                                             gATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTg                                                                                                                                                   >  1:136676/1‑250 (MQ=255)
                                                                             gATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTg                                                                                                                                                   >  1:231823/1‑250 (MQ=255)
                                                                                   aaGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                >  1:19855/1‑248 (MQ=255)
                                                                                    aGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                >  1:32923/1‑248 (MQ=255)
                                                                                              cTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGaa                                                                                                                                   >  1:156385/1‑250 (MQ=255)
                                                                                                       atTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGtt                                                                                                                         >  1:339538/1‑251 (MQ=255)
                                                                                                             gTCGGCGATGTGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCaaa                                                                                                                   <  1:310058/251‑1 (MQ=255)
                                                                                                               cGGCGCTGTGAACTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGCTCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGAATGGAAAGTTTTGAACGGGAATTTATTGAGCAGACCTGCGTGAAACTGGGGGTTGGCAAAgg                                                                                                                 >  2:350714/1‑251 (MQ=255)
                                                                                                                  cgatggggtttgccgacccGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGAGCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAAGTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtgg                                                                                                              <  2:156385/234‑1 (MQ=255)
                                                                                                                   gATGTGATCTACCGGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCACAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtggt                                                                                                             <  1:347054/251‑1 (MQ=255)
                                                                                                                       tcaTCTACCTGAACGGTCCGCTGGAGACCTGCCGCGACGTTTGTAACCGCCGTTTGCTCGAACTGAAACGTGCGATCCATTACGATATCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATAGTGCGCACATACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAAAAACAGACTGCGAAAGGAAAGATTTGGACGTGAATTTATGGAGCAGACAGGCGTTAAACTTGCGGGTGGCCAAGGTGGTaagg                                                                                                         >  2:341397/3‑248 (MQ=255)
                                                                                                                                               tGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                 >  1:302984/1‑251 (MQ=255)
                                                                                                                                                gACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CACCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACACCCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGCGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                 >  1:266067/1‑250 (MQ=255)
                                                                                                                                                  ccgcccgagccgtgggtctccgccgTTTGGTCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCGCATCGTGCGCAAAAACGGCGACAAAGGGGAGATTGTCTCCGGCGGCCCGGCAACCAGTATGCGTATGGAAAGTGTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGa                                                                              <  1:224048/232‑1 (MQ=255)
                                                                                                                                                    tGCCGCGACGTTTGTCACCGCCGTTTGATCGACCTGAAACGTCCGATCCCTTACGCTCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATGGGGCCGCTGACCGAAGAACGCTGCCAGaa                                                                             >  1:151011/1‑250 (MQ=255)
                                                                                                                                                     gCCGCGTCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAt                                                                           <  1:371951/251‑1 (MQ=255)
                                                                                                                                                             gtttgcaaCCGCCGTTGTATCTAACTGAAACGTCCGATCTCTTACGATCACAACGGCAAAGCGATCTTCCACGCTG‑CACCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGATAACCAGTATGCGTATGGAAAGTTTTGAACTTGATTTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGgcg                                                                   <  1:160211/244‑1 (MQ=255)
                                                                                                                                                                                        aaaCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACGGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTGTCCCGGCAGGCTgc                                        <  2:231823/251‑1 (MQ=255)
                                                                                                                                                                                        aaaCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGACCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTCTTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGACGACGGCTGCCAGAAATTCAAGGCGCTACATGGGATTTTCCCGGCAGGCTgc                                        >  1:42769/1‑251 (MQ=255)
                                                                                                                                                                                              ccGATCCCTTACGAGCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCGCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGT‑g                                 <  1:19427/251‑1 (MQ=255)
                                                                                                                                                                                               cgagcccgtACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAagtg                                                                                                               <  2:139689/165‑1 (MQ=255)
                                                                                                                                                                                                    ccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGT‑GCTggcg                           >  1:183788/1‑251 (MQ=255)
                                                                                                                                                                                                      ttACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGG‑GCTggcggc                         >  1:47369/1‑251 (MQ=255)
                                                                                                                                                                                                                aaCGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAGTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGT‑GCTGGCGGCAACCCAGGt                <  2:32923/251‑1 (MQ=255)
                                                                                                                                                                                                                aaCGGCAAAACGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACATCCAGTATGCGTATGGAAAGATTTGAACGTGAATTTATTGCGCAGACCGGCGTGAGACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACAGAAGAAGTCTGCCAGAAATTCAAGGCGCGACCTGTGATTTTCCCGGCCGTCAGGGCGGTTGC‑GGCGGCAACCAAGGTg               >  2:23576/1‑251 (MQ=255)
                                                                                                                                                                                                                 aCGGCAAAGCGATTTTCCACGCTG‑CACCATCGTGCGAAAAACCGGCGACAAATAGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAACGATTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCTGGCTGCGCGGT‑GCTGGCGGCAcccccagg                >  2:380579/1‑245 (MQ=255)
                                                                                                                                                                                                                      aaaGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGT‑GCTGGCGGCAACCCAGGTGGAagag         <  1:266378/251‑1 (MQ=255)
                                                                                                                                                                                                                              tttCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGACCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATAGAGCAGACCGGAGAGAAACTGGTGGTTGGCAAAGGTGGAATGGGGCCGCTGACCGAAGAAGGCTGGCAGGACTTAAAGGCGCTACATGTGATTTTACCGGCAGGGTGCGCGGT‑GCTGGCGGCCCCCCAGGTGGGAGAGATTgaaa  >  2:148947/1‑249 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
GCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATC‑AAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGT‑GCTGGCGGCAACCCAGGTGGAAGAGATTGAAG  >  NC_000913/3207317‑3207788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑