Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,602	2 bp→TT	intergenic (‑80/‑130)	ettA ← / → slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,602	0	C	T	100.0%	124.5 / NA	40	intergenic (‑80/‑131)	ettA/slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/17);  total (23/17)
*	NC_000913	4,630,603	0	C	T	100.0%	131.9 / NA	40	intergenic (‑81/‑130)	ettA/slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/17);  total (23/17)

GCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTC‑GATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACG  >  NC_000913/4630365‑4630844                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                   gCCAAAATGCGCAGCAGGGTAGACTTACCCGCTCCGGTCAGACCCAGTACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGTTATAAACGAAGTGAGCCACGTTGGACTTTGCCTCTATGTTTTCCGTGATGATGAGTTTTCAAAGGTGAAGTGTAGTCTTTTTCCCCTCCTAATCCCAGTTGGTC‑GATcgcct                                                                                                                                                                                                                                       <  1:396473/251‑5 (MQ=255)    aTAAGGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCg                                                                                                                                                                                                                                    <  1:258619/251‑1 (MQ=255)                      gACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACATTTCGGCGGAACAACTTTTCCGACACGATGCATGGTATAAACGGATTGATCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGAGAAGCCTTTTTCCCCGCCTAATCCCATTTGGTC‑GATCACCTTCGCcgtaaaagtactaca                                                                                                                                                                                                                    >  2:118937/1‑244 (MQ=255)                               gcgcCGTTCAGACCCAGGACACCAATTTTGGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAAc                                                                                                                                                                                                         <  1:155486/251‑1 (MQ=255)                                   cgttCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTgg                                                                                                                                                                                                     <  1:156911/249‑1 (MQ=255)                                                           ttGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAg                                                                                                                                                                              <  1:258384/250‑1 (MQ=255)                                                             gCCCCAGGGAAGAAACTCAAAGAGATGTTTTGCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCAt                                                                                                                                                                           <  2:407691/251‑1 (MQ=255)                                                              ccccAGGGCAGACACTCAGAGAGATGTTTTTCAAAATATGACGCTTCGGCGGAACAAATTTGCCGACACGATGCATGGTATAAACGAATTGTGTCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGGGTTTTCAACGGCGAAGTGTAGCCTTTTTCCCCGCCTATTCCCAGTTGGTC‑GCTCACCTTCGCAGTAGATGTAAAAAAATATACGTAATGTTGCGTAAACGGCatttattggctcaaat                                                                                                                                                                           >  2:342286/1‑235 (MQ=255)                                                                  aGGGAAGAAACTCAGAAAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACATTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGAATTCGCCTCTATGTTTATCGTGATGATGAGTTTTCCAAGGCGAAGTGTAGCCTTTTTCCACGCATAATCCCAGTTGGTCTGA‑AAGCTTCGCAGTAAAAGTCAAAACGAGACCGTAACGGGGAGTAAACGGCAATGCCGGGATAGCATAAAtt                                                                                                                                                                      >  2:40548/1‑250 (MQ=255)                                                                          aaCTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAcgc                                                                                                                                                              >  1:231723/1‑251 (MQ=255)                                                                               agagagATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCAt                                                                                                                                                         <  1:228829/251‑1 (MQ=255)                                                                               agagagATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCAt                                                                                                                                                         >  1:369971/1‑251 (MQ=255)                                                                                gtgagaTGTATTTCAAAAAATTCCGTTTCGGCGGCACCAATTGGCCCGCACGAGGCATGGTATAAACTAATTGAGCCACTTTGGTCTTCGCCTTTATGTTTATCGTGAGGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGGC‑GATCAACGTCGCATTAAAAGTAAAAAAGTTTCCGTAACGTCGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTTCGCGGCAtt                                                                                                                                                        <  2:281871/249‑2 (MQ=255)                                                                                   ggaTGTTTTTCAAAATAGGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACg                                                                                                                                                     <  2:125254/250‑1 (MQ=255)                                                                                     aTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGa                                                                                                                                                                                      >  1:360369/1‑216 (MQ=255)                                                                                     aTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGAATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGa                                                                                                                                                                                      <  2:360369/216‑1 (MQ=255)                                                                                                 atatGACGTTTCGGCGGAATCTCTTGGCCGACTCGATGCATGGTACAATCGAATGTAGCCACGGTGGACTTTGCCTCTATGTTTTTCGTGAAGATGAGTTTTCAAAGGCGAAGTTTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTGCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGTCGCTGCATTGATGTAt                                                                                                                                       <  2:406708/251‑1 (MQ=255)                                                                                                          ttCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGCATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACtt                                                                                                                               >  2:41574/1‑250 (MQ=255)                                                                                                          tcgggcggaaatactgggCCGACACGATGCATGGGATAAACGAATTGAGCCACGTTGGACTGCGCCTCTATGTTTATCGGGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTATCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACtt                                                                                                                               <  2:436644/233‑1 (MQ=255)                                                                                                                 gAACAACTTTGCCGACACGATGCATGGTATAAACGAATTAAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGCTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCATCGCAGTAAAAGGAAAAAAGAGTCCGTAACGTGGCGTAAACGGCAGTGAATGGTTAGCATAAATCTATTACGCGGAATGACGCTGCATTGATGTATTTACACTTAAAGGATg                                                                                                                       >  2:121352/1‑251 (MQ=255)                                                                                                                    caacTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCAccttc                                                                                                                                                                                                                                     <  2:264334/138‑4 (MQ=255)                                                                                                                    caacTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCAccttc                                                                                                                                                                                                                                     >  1:264334/1‑135 (MQ=255)                                                                                                                        tttGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTtgt                                                                                                                >  1:474894/1‑251 (MQ=255)                                                                                                                             cGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaa                                                                                                            >  1:222181/1‑250 (MQ=255)                                                                                                                               acacGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaaaa                                                                                                          >  2:265696/1‑250 (MQ=255)                                                                                                                                     tGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTACCGTGGCGTAACCGGCAATGACTGGTTAGCATAAATCTATTACGCGGCCTGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCaa                                                                                                    >  2:287415/1‑250 (MQ=255)                                                                                                                                                 aCGAATTGAGCCACGTTGGACTTCGCATCTATGTTTATCGTGATGATGAGGTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGAATTTATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTgg                                                                                       >  2:283020/1‑251 (MQ=255)                                                                                                                                                                  ggccTTCGCCTCTATGTTTATCGTGATGATGAGGTTTCAAAGGCGAAGTGGTGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCg                                                                       <  2:51386/247‑1 (MQ=255)                                                                                                                                                                         gCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAAAGGCAATGACTGGTTAGCATAAATCTATTAAGCGGCATGACGCTGCATTGCTGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGtgtctgt                                                               >  1:206177/1‑251 (MQ=255)                                                                                                                                                                          ccTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGtgtctgtc                                                              >  1:334330/1‑251 (MQ=255)                                                                                                                                                                                tGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGGGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTACAAGTAAAAACGTGTCCGTAACGTGGCGTAACCGGCCATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGATTGTGGAAAAAGCCAAACAAGTTACATGGCGGCTGTTGGCTGCCGGTGTCTGTctgcagc                                                        >  2:292907/1‑248 (MQ=255)                                                                                                                                                                                     aTCGTGATGATGAGTTTTCAAAGGCGAAGGGGAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGt                                                    <  2:128301/250‑1 (MQ=255)                                                                                                                                                                                     aTCGTGATGATGAGTTGTCAAAGGCGCAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGAGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCCTTGATGTATTTACACTTAGCGGATGAGCTTGTGGAAAAAGCCAAACAAGATACCTAGAGGCTGTTGGCTGCCGGAGTCTGTCTGATGACGGTc                                                   >  1:352586/1‑251 (MQ=255)                                                                                                                                                                                        gTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTcagc                                                >  1:420723/1‑251 (MQ=255)                                                                                                                                                                                           atgaTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCagcagc                                             >  2:418181/4‑251 (MQ=255)                                                                                                                                                                                                          aGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGt                                                                                              <  2:43754/187‑1 (MQ=255)                                                                                                                                                                                                          aGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGt                                                                                              >  1:43754/1‑187 (MQ=255)                                                                                                                                                                                                                         tttttCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATACATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGgc                                        >  2:290072/1‑226 (MQ=255)                                                                                                                                                                                                                         tttttCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTAGTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGgc                                        <  1:290072/226‑1 (MQ=255)                                                                                                                                                                                                                                      aaTCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTAcg  >  1:214617/1‑251 (MQ=255)                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                   GCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTC‑GATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACG  >  NC_000913/4630365‑4630844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑