Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,512,423	2 bp→TT	coding (283‑284/1239 nt)	mntH ←	Mn(2(+))/Fe(2(+)): H(+) symporter MntH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,512,423	0	C	T	100.0%	106.2 / NA	34	W95K (TGG→AAG)	mntH	Mn(2(+))/Fe(2(+)): H(+) symporter MntH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)
*	NC_000913	2,512,424	0	A	T	97.1%	101.1 / ‑6.4	34	W95K (TGG→AAG)	mntH	Mn(2(+))/Fe(2(+)): H(+) symporter MntH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (19/14);  minor base C (1/0);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGAGAAAATCAACTCGACAATGTAAGCCGCGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAAC‑CAATCGCCGCAATGAACGCAGGTCCCATTAATGCG  >  NC_000913/2512180‑2512650                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                    tGGGAGAAAATCAACTCGACAATGTAAGCCGCGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCCGCACCGCGCCCTGCAACAACGAAACACCAAGAATGCGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTA‑AACCCAATAGAACTTCACTAc                                                                                                                                                                                                                               >  2:143020/1‑251 (MQ=255)         aaacAACCCGACAATGTAAGCCGCGGCAACAAACAACAGTACCCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGAcgcg                                                                                                                                                                                                                       <  1:335810/248‑1 (MQ=255)                 cGACAATGTAAGCCGCGGCAAAAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGt                                                                                                                                                                                                                 <  1:203325/249‑1 (MQ=255)                   acaagttAAGCCGCGCAAAAAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATc                                                                                                                                                                                                             <  2:237507/245‑1 (MQ=255)                      aTGTAAGCCGCGGCAACAAACAACCGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAACATCAGGACAGTCGCGATCCCCGTCCGCACCGCGCCCTGCAACAACGAACCACCAAGACTGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTCCACTACGGGACGCGGATAGGGATcgcg                                                                                                                                                                                                          >  2:461607/1‑251 (MQ=255)                                                   cccGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTgg                                                                                                                                                                             >  2:196577/1‑251 (MQ=255)                                                       ccAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAAt                                                                                                                                                                         >  2:152232/1‑251 (MQ=255)                                                              ctttcacaaacGGTTTTTGACCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCACCGTCAGCACCGCGCCCTGCAACAACGAAACAACAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATATGCTCCGCCAGATTTTTACCGGTGGCAATCCATAGt                                                                                                                                                                  >  2:199009/11‑251 (MQ=255)                                                                          gTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTGTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAgg                                                                                                                                                      <  1:38024/251‑1 (MQ=255)                                                                              ttGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCt                                                                                                                                                  >  1:336806/1‑251 (MQ=255)                                                                                gCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGCCGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGGGAGGATCTg                                                                                                                                                 >  2:134068/1‑250 (MQ=255)                                                                                gCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCGGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTg                                                                                                                                                 <  1:27372/250‑1 (MQ=255)                                                                                 cccGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATATTTACCGGTGGCAATCCCTAGTTTGGCCGAGAGGAGCTGa                                                                                                                                                >  2:87008/1‑250 (MQ=255)                                                                                      gACGTTGCAGCATTAAAATCAGGAAAGTCGCGATACCCGTCAGCACCGCGCCCTGCAACAACGAAACCCCAAGAATGAGTTTAACACCGATCGCCGCACAAATAAATTCCGCCAGGACGGTTGCCATCGCAATAGTTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCATTCCCTAGTTTGGCAGAGAGGTCCTGAATCAGc                                                                                                                                          >  2:130545/1‑251 (MQ=255)                                                                                       aCGTTGCAGCATTAAAATCAGGAAAGTCGCGCTCCCCGTCAGCACCGCGCCATGCAACAACGAAACACCAAGAATGAGTTTAACCCCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑ACCCCAATAGACCTTCCCTACGAGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCTATCACTAGTTTGGCAGAGAGGATCTGAATCAGc                                                                                                                                          >  1:231541/1‑250 (MQ=255)                                                                                        cGTTGCAGCATTAAAAGCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCAt                                                                                                                                        <  1:461607/251‑1 (MQ=255)                                                                                              agcaTTAAAATCAGGAAAGTCGCGATCCCCGTCATCACCGCGTCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCAt                                                                                                                                  <  1:233027/251‑1 (MQ=255)                                                                                                      aaTCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTgg                                                                                                                          <  1:76999/251‑1 (MQ=255)                                                                                                       aTCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGc                                                                                                                         >  1:241666/1‑251 (MQ=255)                                                                                                          aGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGccc                                                                                                                       >  1:383767/1‑250 (MQ=255)                                                                                                                     gATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAAccc                                                                                                           <  1:390739/251‑1 (MQ=255)                                                                                                                                gCACCGCGCCCTGCAACAACGAAACCCCAAGAAGGAGTTTAAAACCGATCGCCGCACCAATAAATGCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGa                                                                                                <  2:383767/251‑1 (MQ=255)                                                                                                                                         ccTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAg                                                                                       <  1:367941/251‑1 (MQ=255)                                                                                                                                               acaacGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGc                                                                                  >  1:154433/1‑250 (MQ=255)                                                                                                                                                  acGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCAccc                                                                              >  1:8715/1‑251 (MQ=255)                                                                                                                                                     aaaCACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGcc                                                                           <  1:325344/251‑1 (MQ=255)                                                                                                                                                                     tttAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCa                                                            >  1:350280/1‑250 (MQ=255)                                                                                                                                                                                             aaaTTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAAC‑Ca                                   >  1:119891/1‑250 (MQ=255)                                                                                                                                                                                                 tCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAAC‑CAATcgc                              >  1:278354/1‑251 (MQ=255)                                                                                                                                                                                                              ttGCCATCGCAATAATTTCTGCCTGTAACCCA‑TAGCACTTCACTACGGGACGCGGAGAGGGATCGCGCATCTACTCAGCCAGATTTTTACCGGGGGCCATCCCTAGTTTGGCAGAGAGGATCTGAATCCGCATCGCCCTCCGGTTAGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAACTAGAACCCGCCTGGATATTGGACGCACAGTTCCCGTGCTCGATA‑AACACAATCGCAGCAAtaaagtac                 >  2:453951/1‑244 (MQ=255)                                                                                                                                                                                                                gCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAAC‑CAATCGCCGCAATGAACGCAg                <  1:303860/250‑1 (MQ=255)                                                                                                                                                                                                                      gCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCAccc                                                                              >  2:223072/1‑183 (MQ=255)                                                                                                                                                                                                                      gCAATAATTTCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCAccc                                                                              <  1:223072/183‑1 (MQ=255)                                                                                                                                                                                                                              ttCTGCCTG‑AACCCAATAGAACTTCACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTAACGGGATCGATATAAC‑CAATCGCCGCAATGAACGCAGGTCCCATTAATGCg  >  1:35886/1‑250 (MQ=255)                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                    TGGGAGAAAATCAACTCGACAATGTAAGCCGCGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTCGGTTGCCATCGCAATAATTTCTGCCTG‑AACCCAATAGAACCACACTACGGGACGCGGATAGTGATCGCGAATCTGCTCCGCCAGATTTTTACCGGTGGCAATCCCTAGTTTGGCAGAGAGGATCTGAATCAGCATCGCCATCAGGTTGGCCCAAACGACAACCCACAGTAGCTGATAGCCGAAGCTAGCACCCGCCTGAATATTGGTCGCAAAGTTACCGGGATCGATATAAC‑CAATCGCCGCAATGAACGCAGGTCCCATTAATGCG  >  NC_000913/2512180‑2512650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑