Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,497,3850TC88.9% 68.6 / ‑2.7 27F177S (TTT→TCT) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (12/12);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009131,497,3860T.88.9% 97.5 / 4.4 27coding (531/1656 nt)opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (12/12);  minor base C (2/1);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009131,497,3870CA96.3% 82.7 / NA 27L178N (CTC→AAC) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (14/12);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009131,497,3880TA96.3% 73.6 / ‑5.1 27L178N (CTC→AAC) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (13/13);  minor base G (1/0);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAA  >  NC_000913/1497158‑1497611
                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                 
ggACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCgcgc                                                                                                                                                                                                            >  2:126918/1‑251 (MQ=255)
               ccATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGAGCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCTCCGTTGATGATACat                                                                                                                                                                                              <  1:301888/250‑1 (MQ=255)
                               ttCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCg                                                                                                                                                                             >  1:36620/1‑251 (MQ=255)
                                            ttCGTATGTTTTCTGAAGATCCAGCCAAACATCTGGGGCGTCAACTTCACTTTTGCCCGGCGTTGTTCAAATACAACGATGCAGGTGTTGATGCAAAACCATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCTTCAGCCGCGCGAGTTATTTACGCGCCGTTGTTGGTCCATATCAATACGGTTTGTCGGCACGCggaccgg                                                                                                                                                                >  2:478517/1‑246 (MQ=255)
                                                   gTTTTCTGTAGATCCAGCAACACATCCGGCGCGAGAAATTCAATTTCCCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGTTGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCg                                                                                                                                                          >  1:135134/1‑250 (MQ=255)
                                                            aGATCCAGCAACACATCTGGCGTGTGAAATTCACTTTCGCCCGGATTTGTTCAAATACAACGATGCAGGTGGTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTGTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTTGCGATCGACACTTaca                                                                                                                                                 <  1:339583/250‑1 (MQ=255)
                                                                   gCAACACATCTGGCGCGTGCAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGt                                                                                                                                         >  2:284507/1‑251 (MQ=255)
                                                                      actcaTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTCCAAATACAACGATGCCGGTTTGGATACAAAACAATTAGAAGGTCAAAGCTATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAATCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTAGTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGTCGTTCGACACTTACACCGACAGTaaa                                                                                                                                      <  2:122330/248‑1 (MQ=255)
                                                                       aactactgggccgTGAAATTCACTTTCGCCGGGTGTTGTTCAAATACAACGCTGCAGGTGTTGATACAAAACAATTAGAAGGGAAAAGCGAGCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTAACATCAACGGCGCGAGTTATTTCCGAGCCGTTGATGATACATATCAATACGGTTTGTCGTCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAaag                                                                                                                                     <  2:310147/240‑1 (MQ=255)
                                                                                      aaaTTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTtgat                                                                                                                                                                                                     <  1:147024/172‑1 (MQ=255)
                                                                                      aaaTTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTtgat                                                                                                                                                                                                     >  2:147024/1‑172 (MQ=255)
                                                                                                tCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCACCt                                                                                                             <  1:126918/250‑1 (MQ=255)
                                                                                                         gttgttCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTg                                                                                                    >  1:125233/1‑250 (MQ=255)
                                                                                                                  aTACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTaaa                                                                                          <  1:109760/251‑1 (MQ=255)
                                                                                                                            gCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCg                                                                                                                                                                             <  1:353413/158‑1 (MQ=255)
                                                                                                                            gCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCg                                                                                                                                                                             >  2:353413/1‑158 (MQ=255)
                                                                                                                                  gtTGATACAAAACACTTAGATGGGCACAGCGATCTAGGCTTTCCTGTTTTTCGCGTGTTTAAAGCCCCCGACCTGGTGCGTCTTGATGTAGTATC‑‑TCCACCGCTGCTAGTTATTTCCGCGTCGTTTATGATACATATCAATACGGTTTGTCGTCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTAcc                                                                          <  1:104968/251‑1 (MQ=255)
                                                                                                                                    tGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACAGGGGGCAACTACCtt                                                                        >  1:270318/1‑251 (MQ=255)
                                                                                                                                               aaTTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATg                                                             >  1:148681/1‑251 (MQ=255)
                                                                                                                                                           aaaGCGATCTCGGCTTTGCCGGTTGTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATa                                                 <  1:13582/251‑1 (MQ=255)
                                                                                                                                                               agATCTCGGCTTTGCCGGTTTTAGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCCTCAACGGCGCGAGTTATTTCCGCGCCGTTGAGTATACATATCAATAAGGTTTGTCGGACCGCGGCCTGGCGATCGAAACATCCACCGACAGTACAGCAGCGTTCCCCGACTTTACCCCCTTCTGGTTTGATAAGGTAAAACCGGGAGCAACTACCTGTCCCCTTTATGCGTTGCTCGagagacg                                             >  2:324211/2‑245 (MQ=255)
                                                                                                                                                                           tGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTg                                  <  1:243375/250‑1 (MQ=255)
                                                                                                                                                                                gTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCt                            >  2:135712/1‑251 (MQ=255)
                                                                                                                                                                                        gtgtTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTc                    >  1:463125/1‑251 (MQ=255)
                                                                                                                                                                                         tgtTTAAAGCCCCCGACCTGGCGCGCCGTGATGTATTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCTCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGtt                     <  1:73818/249‑1 (MQ=255)
                                                                                                                                                                                                     ccGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATtgtg       <  1:135712/251‑1 (MQ=255)
                                                                                                                                                                                                           tGGCGCGCCGTGATGTAGTATCATCAACGGCACGAGTAATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTTCCTATAAGTTCACTATCCATTGTGAGaaa  >  1:405289/1‑250 (MQ=255)
                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                 
GGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAA  >  NC_000913/1497158‑1497611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: