Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,975	2 bp→TA	coding (738‑739/954 nt)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,975	0	G	T	96.0%	150.9 / ‑1.7	50	P246P (CCG→CCT)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (22/26);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01
*	NC_000913	8,976	0	G	A	95.9%	139.4 / ‑2.6	50	A247T (GCA→ACA)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (22/25);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

TGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  NC_000913/8732‑9216                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                 tGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGAACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGATTCTGTATATGAAATCTACCAGTACTACCAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGTCTGCGACCGACTTACCATCGCACCTACActgc                                                                                                                                                                                                                                            >  1:465287/1‑251 (MQ=255)      gtgCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGaa                                                                                                                                                                                                                                        >  1:247978/1‑250 (MQ=255)          ggAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAagcgc                                                                                                                                                                                                                                   >  1:202619/1‑248 (MQ=255)                            gATCTCACCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGCAAGAGCTGGctgcaagcgaggggg                                                                                                                                                                                                                 >  2:47307/1‑237 (MQ=255)                               ctcGCCGTTTGTTGGCCGTATTCTTGACTGGTACACAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGCAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCt                                                                                                                                                                                                               >  1:165044/1‑250 (MQ=255)                                 cGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAAGACCGATAAGAAATAGTACGCTCCGGCAGAAGATCCGGGCGTGGTGTCTGTATCTGAAATCTACCAGTACTCCAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATc                                                                                                                                                                                                            <  2:323148/251‑1 (MQ=255)                                  gCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAaga                                                                                                                                                                                                                                     <  2:253323/225‑1 (MQ=255)                                  gCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAaga                                                                                                                                                                                                                                     >  1:253323/1‑225 (MQ=255)                                            ggCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACt                                                                                                                                                                                                 <  1:220604/251‑1 (MQ=255)                                                  aTTCTTGCCTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCGCCTGCAGAAGATCCGGGCGTGGTGTCGGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCTACCGTCTTCCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAATGGGCTATCGAACGTAAACTGTCtt                                                                                                                                                                                            <  2:186177/250‑1 (MQ=255)                                                          cTGGTACAAAGCGAACACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCg                                                                                                                                                                                   >  1:90466/1‑251 (MQ=255)                                                               aCAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTg                                                                                                                                                                              <  2:90466/251‑1 (MQ=255)                                                                  aaGCGAAGACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTTCTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGaaa                                                                                                                                                                           <  2:195151/251‑1 (MQ=255)                                                                        aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGAGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGATATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCCACTACACTGCTGAAAGAGCTGGCTGGGCGGGAAGGGGCTATCGAACGTTAACTGTCTTACACCGGCGAGGTGAAATcgcgc                                                                                                                                                                     >  2:193141/1‑250 (MQ=255)                                                                            cGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCgg                                                                                                                                                                 <  2:436554/251‑1 (MQ=255)                                                                             gATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCCCTGCTGAAAGAACTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAATGAAAGCGCGTCCGgc                                                                                                                                                                >  2:191174/1‑251 (MQ=255)                                                                              aTAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGCGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGCCCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAAGGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGGGCGGCCGgcg                                                                                                                                                               >  2:25694/1‑251 (MQ=255)                                                                              aTAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACCGTCTTACACCGGCGAAGTGAAAGCGAGTCCGgcg                                                                                                                                                               >  2:421796/1‑251 (MQ=255)                                                                                   aaagaGTACGCTTCCGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAATAGCACTGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACTTCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCTCACCTTCACGGCTTAAAGAGCTCGCTGAGAGCTAATTGTCTATCGAACGTAAACTGTCTTACACCGTCGAAGTGAAAGCTCGTCCGGCGCTTAt                                                                                                                                                          <  1:298982/251‑1 (MQ=255)                                                                                   aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCCCCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTAt                                                                                                                                                          <  1:298987/251‑1 (MQ=255)                                                                                   aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTAt                                                                                                                                                          <  1:476703/251‑1 (MQ=255)                                                                                               ccGGCAGAATAGCCGGGCGTGGTTTCTGTATCTGAAAGCTACCAGTACTTCAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGa                                                                                                                                              <  2:326150/251‑1 (MQ=255)                                                                                                 gggagaagaGCCGGGCGTGGTGTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                                            <  2:26130/248‑1 (MQ=255)                                                                                                 ggCAGAAGATCCGGACGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGAAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                                            >  1:140505/1‑251 (MQ=255)                                                                                                                       tCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAgg                                                                                                                       <  2:251446/250‑1 (MQ=255)                                                                                                                                taaaTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCTCAAGCTTCCGTAACAACGGCGAAATCCTGGAACTGGCAGGCTGCGGCCGTCGTACCATCGCACCTACACTGCGGAAAGAGCGGGCTGAGAGCGAAGGGGCTATCGAACGGAATCTGTCTGACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGGCCGAGTTCCTGTGGCAGCACAACGAGTATCCAATg                                                                                                               <  2:244096/248‑1 (MQ=255)                                                                                                                                   aTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCCGCACAACCAGGATCCAATGGCAGt                                                                                                          >  1:68599/1‑251 (MQ=255)                                                                                                                                       aCCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTa                                                                                                         <  1:21200/248‑1 (MQ=255)                                                                                                                                             actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCACGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGCAGGGGCTATCGAACGTAACCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTg                                                                                                >  2:34408/1‑251 (MQ=255)                                                                                                                                             actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGCCCGTCTTACCATCGCACCTACCCTGCTGAAAGAGCTGGCTGAGAGAGAAGGCGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGGTCCCATGGCAATAGATAAACTg                                                                                                >  2:186442/1‑251 (MQ=255)                                                                                                                                                 cAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCgg                                                                                            >  1:102627/1‑251 (MQ=255)                                                                                                                                                             ttATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                                                                                                                          >  1:20434/1‑161 (MQ=255)                                                                                                                                                             ttATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                                                                                                                          <  2:20434/161‑1 (MQ=255)                                                                                                                                                              taggAAACCGTGGTTATGGGTGCAAGCTTCCGTAACATCGGCGAAATTCAGGAACTGGCAGGCTGCGGCCGTCTTACCATCTCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGTCGATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACATCCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATccttt                                                                                <  2:159300/247‑4 (MQ=255)                                                                                                                                                                 gAAACCGTGGTTATTGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGtt                                                                            <  1:267424/251‑1 (MQ=255)                                                                                                                                                                         ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCCCAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAATTTGCTAttt                                                                    >  2:117261/1‑250 (MQ=255)                                                                                                                                                                         ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                                                    <  2:214487/251‑1 (MQ=255)                                                                                                                                                                         ggTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTg                                                                    >  1:91547/1‑251 (MQ=255)                                                                                                                                                                             aTGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAcc                                                                 <  1:390076/250‑1 (MQ=255)                                                                                                                                                                                  cgcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                                           <  1:117261/251‑1 (MQ=255)                                                                                                                                                                                   gcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTCCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCGTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGGTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGGGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaa                                                          <  2:394905/251‑1 (MQ=255)                                                                                                                                                                                                             tCTGGAACTGTCATGCTGCGTCCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGGTCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCTGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctg                                 <  1:218888/250‑1 (MQ=255)                                                                                                                                                                                                                   aCTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGCGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                  >  1:164552/1‑243 (MQ=255)                                                                                                                                                                                                                   aCTGGCAGGCTGCGACCGTCGTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGGTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                  <  2:164552/243‑1 (MQ=255)                                                                                                                                                                                                                      ggCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCa                        <  1:265414/250‑1 (MQ=255)                                                                                                                                                                                                                                     tcttaCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAATGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCgg        >  2:332863/5‑251 (MQ=255)                                                                                                                                                                                                                                        taCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGa      >  1:142557/2‑250 (MQ=255)                                                                                                                                                                                                                                         aCCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGa      >  1:234137/1‑249 (MQ=255)                                                                                                                                                                                                                                           cATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTc  <  2:102627/251‑1 (MQ=255)                                                                                                                                                                                                                                           cATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATACTTCTTAACGTGACCGGGCAGTc  >  2:135568/1‑251 (MQ=255)                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                 TGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTC  >  NC_000913/8732‑9216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑