Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,624 C→G L112L (CTG→CTC)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,6240CG96.7% 90.3 / ‑5.6 30L112L (CTG→CTC) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (15/14);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGG‑TAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTT‑AAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAA  >  NC_000913/7387‑7859
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
cagcagAGCAAAGACAGCGAGAATAATACCTGTCCCCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCgg                                                                                                                                                                                                                                  <  2:331976/251‑1 (MQ=255)
 agcagAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGCAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGc                                                                                                                                                                                                                                 >  2:92594/1‑251 (MQ=255)
         aaaGACAGCTAGAATAATACCTGTCACCCGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGTCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAgg                                                                                                                                                                                                                         <  2:204770/251‑1 (MQ=255)
                 cGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAACACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCAATg                                                                                                                                                                                                                 >  2:190682/1‑251 (MQ=255)
                    gaaaaataccTGCCACCCGCGGGGGAAAAGCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTGCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCg                                                                                                                                                                                                              <  2:3472/243‑1 (MQ=255)
                    gaATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCg                                                                                                                                                                                                              >  1:271435/1‑251 (MQ=255)
                                                    aaaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGTCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCAAATACCAAGCCGTTACGCCACGAAACTGCCCATTGGCATCACGTTCATTATAAAGCTGAGCGAGGGAAAATTCGGCAACCGCGGTAGCCATGCCGGTAAACGCGGAAAccccacaccagaaacc                                                                                                                                                                                 >  2:220542/1‑235 (MQ=255)
                                                                aGGGCGCGCGAAACGGCGTGCGTCTGAATTCTGCTGAAAATTAGGCCGTAGGCGATGAGAAAAAAGCCGGCAAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATATCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCAcc                                                                                                                                                                  <  2:93977/251‑1 (MQ=255)
                                                                        cgaaaCGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑Taa                                                                                                                                                         <  2:411608/248‑1 (MQ=255)
                                                                                         gaattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATTTACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCCGCGCAACGCCg                                                                                                                                        >  1:381316/5‑251 (MQ=255)
                                                                                          aattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGGCCGCGCAAcccccg                                                                                                                                       >  1:456193/4‑246 (MQ=255)
                                                                                                   gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGTAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcgg                                                                                                                                                                <  1:235609/218‑1 (MQ=255)
                                                                                                   gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAAACCACATCCAGAAGACGGCTCCAGGTCCACcgg                                                                                                                                                                >  2:235609/1‑218 (MQ=255)
                                                                                                    aaaaTTAGGCCGTAGGCGATGAGAAAAAAGACGCAGAACAGAACCCCCCTCCAGCGCATCCCCCGCCCGCGCCCCATATACCATGCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCGTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGcc                                                                                                                             <  2:393739/251‑1 (MQ=255)
                                                                                                              cgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAAATTCCCAGGGCACCGCCGGCCAgttaccgtctcccacc                                                                                                                    >  1:244572/3‑236 (MQ=255)
                                                                                                                   gCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGAGCAACGCCAGCCAGGGTGCCGCAAcccccccggcc                                                                                                              >  1:347794/1‑243 (MQ=255)
                                                                                                                    cGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑GAATTGCCAGCGCAACCCCGGCCAGGTGGCCGCTccccccccccgcc                                                                                                             >  2:310622/1‑242 (MQ=255)
                                                                                                                             aaaaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAACGCCACCCCGGCCAGGCTGCCGCCACCCCcgcggcccccccaaccg                                                                                                    >  2:179556/1‑238 (MQ=255)
                                                                                                                               aaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCCGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCCCCAGACTggg                                                                                                  >  1:443645/1‑250 (MQ=255)
                                                                                                                                   cGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTacac                                                                                              <  1:92594/251‑1 (MQ=255)
                                                                                                                                        aacaaaacccccccccAGCGCACCCCCAGCCCGCGCCCCATTTCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGa                                                                                         <  2:381316/237‑1 (MQ=255)
                                                                                                                                              aCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCACGACTTGTCCACAAAGACTgga                                                                                    >  1:149829/1‑248 (MQ=255)
                                                                                                                                                         gcgaaTCCCCAGCCCGCGCGCCATATCCCATGTCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAACCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGG‑TAATAGCCAGCGCAACGCCGGCCAGGTTGACGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAAc                                                                        <  1:15719/247‑1 (MQ=255)
                                                                                                                                                           ccaaccccCGCCCGCGCCCCAAATCCAATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCg                                                                      <  2:119920/247‑1 (MQ=255)
                                                                                                                                                              tCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCCGGGCAAAACCGGCCAGGGTGCCGCCACCCCCCCCCGCCGCCAGCCTGGTGCACAATGAAAGAAATGAGGGTAAACCCCCt                                                                   >  2:216041/1‑251 (MQ=255)
                                                                                                                                                                  cagcccccccccaatatACCATGCCGGCCCCCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAACCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCt                                                               <  1:128903/238‑1 (MQ=255)
                                                                                                                                                                      ccgcccgcCATATCCCAGGCCGGCCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTAAAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTgg                                                           <  1:310622/251‑1 (MQ=255)
                                                                                                                                                                               atatCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCt                                                  <  1:245681/251‑1 (MQ=255)
                                                                                                                                                                               aaatcccaggCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACt                                                                                       <  1:325746/205‑1 (MQ=255)
                                                                                                                                                                                                                               ttATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCGCAAGACTGGTGCACAAAGACTGAAATGAGGTTAAACCGCCTGGGTGTGGGGGGAGGCTATTTTTTAATA‑TTTTTCCAAACTGGCGGGTGTAgggcaaaccgcaaa  >  2:238255/1‑237 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
CAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGG‑TAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTT‑AAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAA  >  NC_000913/7387‑7859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑