Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,618 T→C Q114Q (CAA→CAG)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,6180TC96.8% 92.3 / ‑6.2 31Q114Q (CAA→CAG) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (15/15);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGG‑TAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTT‑AAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAA  >  NC_000913/7381‑7859
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGGCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgagggaa                                                                                                                                                                                                                                         <  1:239638/246‑8 (MQ=255)
      cagcagAGCAAAGACAGCGAGAATAATACCTGTCCCCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCgg                                                                                                                                                                                                                                  <  2:331976/251‑1 (MQ=255)
       agcagAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGCAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGc                                                                                                                                                                                                                                 >  2:92594/1‑251 (MQ=255)
               aaaGACAGCTAGAATAATACCTGTCACCCGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGTCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAgg                                                                                                                                                                                                                         <  2:204770/251‑1 (MQ=255)
                       cGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAACACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCAATg                                                                                                                                                                                                                 >  2:190682/1‑251 (MQ=255)
                          gaaaaataccTGCCACCCGCGGGGGAAAAGCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTGCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCg                                                                                                                                                                                                              <  2:3472/243‑1 (MQ=255)
                          gaATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCg                                                                                                                                                                                                              >  1:271435/1‑251 (MQ=255)
                                                          aaaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGTCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCAAATACCAAGCCGTTACGCCACGAAACTGCCCATTGGCATCACGTTCATTATAAAGCTGAGCGAGGGAAAATTCGGCAACCGCGGTAGCCATGCCGGTAAACGCGGAAAccccacaccagaaacc                                                                                                                                                                                 >  2:220542/1‑235 (MQ=255)
                                                                      aGGGCGCGCGAAACGGCGTGCGTCTGAATTCTGCTGAAAATTAGGCCGTAGGCGATGAGAAAAAAGCCGGCAAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATATCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCAcc                                                                                                                                                                  <  2:93977/251‑1 (MQ=255)
                                                                              cgaaaCGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑Taa                                                                                                                                                         <  2:411608/248‑1 (MQ=255)
                                                                                               gaattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATTTACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCCGCGCAACGCCg                                                                                                                                        >  1:381316/5‑251 (MQ=255)
                                                                                                aattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGGCCGCGCAAcccccg                                                                                                                                       >  1:456193/4‑246 (MQ=255)
                                                                                                         gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGTAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcgg                                                                                                                                                                <  1:235609/218‑1 (MQ=255)
                                                                                                         gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAAACCACATCCAGAAGACGGCTCCAGGTCCACcgg                                                                                                                                                                >  2:235609/1‑218 (MQ=255)
                                                                                                          aaaaTTAGGCCGTAGGCGATGAGAAAAAAGACGCAGAACAGAACCCCCCTCCAGCGCATCCCCCGCCCGCGCCCCATATACCATGCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCGTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGcc                                                                                                                             <  2:393739/251‑1 (MQ=255)
                                                                                                                    cgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAAATTCCCAGGGCACCGCCGGCCAgttaccgtctcccacc                                                                                                                    >  1:244572/3‑236 (MQ=255)
                                                                                                                         gCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGAGCAACGCCAGCCAGGGTGCCGCAAcccccccggcc                                                                                                              >  1:347794/1‑243 (MQ=255)
                                                                                                                          cGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑GAATTGCCAGCGCAACCCCGGCCAGGTGGCCGCTccccccccccgcc                                                                                                             >  2:310622/1‑242 (MQ=255)
                                                                                                                                   aaaaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAACGCCACCCCGGCCAGGCTGCCGCCACCCCcgcggcccccccaaccg                                                                                                    >  2:179556/1‑238 (MQ=255)
                                                                                                                                     aaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCCGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCCCCAGACTggg                                                                                                  >  1:443645/1‑250 (MQ=255)
                                                                                                                                         cGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTacac                                                                                              <  1:92594/251‑1 (MQ=255)
                                                                                                                                              aacaaaacccccccccAGCGCACCCCCAGCCCGCGCCCCATTTCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGa                                                                                         <  2:381316/237‑1 (MQ=255)
                                                                                                                                                    aCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCACGACTTGTCCACAAAGACTgga                                                                                    >  1:149829/1‑248 (MQ=255)
                                                                                                                                                               gcgaaTCCCCAGCCCGCGCGCCATATCCCATGTCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAACCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGG‑TAATAGCCAGCGCAACGCCGGCCAGGTTGACGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAAc                                                                        <  1:15719/247‑1 (MQ=255)
                                                                                                                                                                 ccaaccccCGCCCGCGCCCCAAATCCAATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCg                                                                      <  2:119920/247‑1 (MQ=255)
                                                                                                                                                                    tCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCCGGGCAAAACCGGCCAGGGTGCCGCCACCCCCCCCCGCCGCCAGCCTGGTGCACAATGAAAGAAATGAGGGTAAACCCCCt                                                                   >  2:216041/1‑251 (MQ=255)
                                                                                                                                                                        cagcccccccccaatatACCATGCCGGCCCCCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAACCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCt                                                               <  1:128903/238‑1 (MQ=255)
                                                                                                                                                                            ccgcccgcCATATCCCAGGCCGGCCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTAAAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTgg                                                           <  1:310622/251‑1 (MQ=255)
                                                                                                                                                                                     atatCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCt                                                  <  1:245681/251‑1 (MQ=255)
                                                                                                                                                                                     aaatcccaggCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACt                                                                                       <  1:325746/205‑1 (MQ=255)
                                                                                                                                                                                                                                     ttATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGG‑TAATTGCCAGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCGCAAGACTGGTGCACAAAGACTGAAATGAGGTTAAACCGCCTGGGTGTGGGGGGAGGCTATTTTTTAATA‑TTTTTCCAAACTGGCGGGTGTAgggcaaaccgcaaa  >  2:238255/1‑237 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
GATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGG‑TAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTT‑AAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAA  >  NC_000913/7381‑7859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑