Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,131	T→C	L64L (TTG→CTG)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,131	0	T	C	100.0%	94.4 / NA	31	L64L (TTG→CTG)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/11);  total (20/11)

GACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGC  >  NC_000913/4640894‑4641377                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       gACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCACTATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGCACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCACCCCCCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGtt                                                                                                                                                                                                                                           >  2:214135/1‑251 (MQ=255)       gATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACg                                                                                                                                                                                                                                      >  2:403231/1‑250 (MQ=255)             cctgTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATgacg                                                                                                                                                                                                                                >  2:339142/4‑247 (MQ=255)                                                           cGATTATTCTGGACGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGTACTGCGGATTGTCGATAGTCAGGCACACCTGGACCCAGCCACCCGCTGGGTCGCCCATGGATCTGGTGATATTTTTGATAATATTAAAGTTTTCCCGACACTGGCTGCATCGTTACACGATTTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCCTtac                                                                                                                                                                                 >  1:77491/1‑251 (MQ=255)                                                                    tGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGCGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGCTAATATTACAGTTTTCCCGACACTGGCTGCATCGTTACACGATGTCGATTTCACTGTCGACACCACTGCGCGCCGTCGGGCGAAATCTCATTACTacgcttcc                                                                                                                                                                        >  2:310796/1‑247 (MQ=255)                                                                         gCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGCTGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCCTTACTacgctccgcccgt                                                                                                                                                                   >  2:27132/1‑242 (MQ=255)                                                                                 cAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGCTGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACCCCAGTCGAACTgg                                                                                                                                                           >  2:267184/1‑251 (MQ=255)                                                                                      cAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCg                                                                                                                                                      >  2:161820/1‑251 (MQ=255)                                                                                       aGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCAGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGc                                                                                                                                                     >  1:300111/1‑251 (MQ=255)                                                                                           aaTAGTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGtt                                                                                                                                                 <  2:17278/251‑1 (MQ=255)                                                                                                             cgGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATg                                                                                                                               >  1:283627/1‑251 (MQ=255)                                                                                                                    tGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCAAACCTGGAGCCAGCCACCAGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTCCCGCTGTTAGAGGAAAAATCTTCATGGATGAGc                                                                                                                        >  2:303379/1‑251 (MQ=255)                                                                                                                       aaaCGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCACCCCGCTGGGTCGCACATGGATTTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCAt                                                                                                                     <  1:147712/251‑1 (MQ=255)                                                                                                                          cGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGcc                                                                                                                  <  1:161820/251‑1 (MQ=255)                                                                                                                                          acTGCGGATTGTCGAGAGGCAGGCAAACCGGGAGCCAGCCACCCGCTGGGTCGCTAATGGAGCTGGTGATATTATTGATAATATTAAAGTTTCCCCGACACTGGCTGAAGCGTTACACGATGTCGATTTCACTGGCGCCACCACTGCGCGCAGTCGGGCGAAAGATCATTACTACGCTACGCCAGTAGAACTGGGACCGCTGTTAGAGGAAAAATCTTTATGGATGAGCGATGCAGCGCTGGTGGTTGGTc                                                                                                  <  2:32266/250‑1 (MQ=255)                                                                                                                                                    gTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATg                                                                                                                    >  1:407366/1‑223 (MQ=255)                                                                                                                                                    gTCGATAGTCAGGCACACCGGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTc                                                                                        <  1:23754/251‑1 (MQ=255)                                                                                                                                                      cGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCg                                                                                      <  1:397936/251‑1 (MQ=255)                                                                                                                                                                             cagccaCCCGCGGGGTCGCACATGGATCGGGTGATATTATTGATAACATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGACTCCGGGCTGACTAACGAAGAGTTAGCg                                                               <  2:77491/251‑1 (MQ=255)                                                                                                                                                                                       cTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCCGTAGAACTGGTACCGCTGTTCGCGGAAAAATCTTCATGGATGCGCCATGCCGCGCTGGTGTTTGGTAGCGAAGATTCCGGGCTTACTAACGAAGAGTTAGCGTTGGCTGACg                                                     >  2:158839/1‑251 (MQ=255)                                                                                                                                                                                           gTCGCACATGGAGCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCt                                                 <  1:214135/251‑1 (MQ=255)                                                                                                                                                                                               cacaTGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACt                                             >  1:352607/1‑251 (MQ=255)                                                                                                                                                                                                    ggATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGgtgt                                        <  1:339142/251‑1 (MQ=255)                                                                                                                                                                                                              atatTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACCCGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGAtggtg                              <  2:283218/251‑1 (MQ=255)                                                                                                                                                                                                                 ttattGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGc                            >  1:160643/1‑250 (MQ=255)                                                                                                                                                                                                                             ttAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGGAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTAc                                              >  1:205480/1‑220 (MQ=255)                                                                                                                                                                                                                             ttAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGGAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTAc                                              <  2:205480/220‑1 (MQ=255)                                                                                                                                                                                                                             ttAAAGTTTTCACGACACTGGCTGAATCGTTACACGATGTCGATTTAACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGGGGTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGGGGCAGATTATCCTTCg               >  2:418016/1‑251 (MQ=255)                                                                                                                                                                                                                                        ccGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTggg    >  1:148413/1‑251 (MQ=255)                                                                                                                                                                                                                                        ccGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTggg    >  1:59834/1‑251 (MQ=255)                                                                                                                                                                                                                                          gACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGc  >  1:142156/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       GACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGC  >  NC_000913/4640894‑4641377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑