Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,640,908	G→T	intergenic (+137/‑34)	ytjE → / → yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,640,908	0	G	T	100.0%	107.5 / NA	35	intergenic (+137/‑34)	ytjE/yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/15);  total (20/15)

AATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACG  >  NC_000913/4640672‑4641149                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  aatCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTAttt                                                                                                                                                                                                                                     >  1:283218/1‑251 (MQ=255)        cTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCa                                                                                                                                                                                                                                >  1:77121/1‑249 (MQ=255)                atcatACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTgg                                                                                                                                                                                                                      >  1:371853/1‑251 (MQ=255)                 tcatACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCCACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTACGCTAAGCAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGc                                                                                                                                                                                                                     >  2:453603/1‑251 (MQ=255)                        aCTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGc                                                                                                                                                                                                              <  1:262407/251‑1 (MQ=255)                              aaaCGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATaa                                                                                                                                                                                                        <  2:77121/251‑1 (MQ=255)                               aaCGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAAc                                                                                                                                                                                                       <  1:31616/251‑1 (MQ=255)                                   tggtgAATCCAATTGTCGAGATTTATTTTTTATAAAATTAACCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTCCAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTATAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGatt                                                                                                                                                                                                   >  1:422689/1‑251 (MQ=255)                                                   cGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCg                                                                                                                                                                                   >  1:437644/1‑251 (MQ=255)                                                                    aaaaTTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATAtt                                                                                                                                                                  >  2:99606/1‑251 (MQ=255)                                                                         tATCCTAAGGAAACAGAAGGATATATGGCATTATTTAACAATACAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTGAGGATT‑GTGAAATTCGCACCGCGAAATAAGTAGACAGTGATCACCCTTTTCGCGGTTATTTGTTCAAGAAGAGTGGCAAAATGCGTAGAAGGATTATTCTGGGCTCACCCGCCAGAGCAGAAAATATTgggg                                                                                                                                                              <  2:246658/250‑1 (MQ=255)                                                                             cTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCACCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAgcg                                                                                                                                                         >  2:397936/1‑251 (MQ=255)                                                                                     aCAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGc                                                                                                                                                 <  1:466974/251‑1 (MQ=255)                                                                                             atttGTAGCATTTTTGAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTCCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCCCCGCGTAATAAGTTGACATTGATCACCCTGGTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTTTAACGATTATTCTGGTCGCGCCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGaaaa                                                                                                                                         <  2:39995/248‑1 (MQ=255)                                                                                                             aacaatTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgggg                                                                                                                                                              >  1:56576/1‑214 (MQ=255)                                                                                                             aacaatTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTGGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgggg                                                                                                                                                              <  2:56576/214‑1 (MQ=255)                                                                                                                    cAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCgg                                                                                                                  <  1:303594/251‑1 (MQ=255)                                                                                                                      aCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGAt                                                                                                                >  1:19796/1‑251 (MQ=255)                                                                                                                                   tCAGGAACTAATTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTCAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGCCAGTGATCACCCTGTTCGCGGATATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGACACTATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAgac                                                                                                     >  2:438371/1‑247 (MQ=255)                                                                                                                                               ttAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTAAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGa                                                                                        <  2:371853/250‑1 (MQ=255)                                                                                                                                                  gCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGagcc                                                                                     >  2:150182/1‑250 (MQ=255)                                                                                                                                                   ccTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCCCCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGagccag                                                                                   <  1:150182/251‑1 (MQ=255)                                                                                                                                                       ttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGTGGCAGCGGCGCGGGCACTGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAggaccccc                                                                                            >  2:399484/1‑232 (MQ=255)                                                                                                                                                       ttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACAcc                                                                                            <  1:399484/238‑1 (MQ=255)                                                                                                                                                                  gTAAAGGGCTTTTTCTGAGCCTTCCGATAAGGATTTGC‑GAATTCGCACCGCGTAATAAGTCGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAACATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGGAAATATTGGGGCAGCGGCGCGGGCGATGAAAACGATGGGTTATCGCGTACTGCGGATTGTCGTTAGCCAGGCACACCTGGAGCAAGCCACCCGCTggggcg                                                                    >  2:82576/1‑248 (MQ=255)                                                                                                                                                                                 tGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGcgcg                                                                                                                                                    <  2:260945/156‑1 (MQ=255)                                                                                                                                                                                 tGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGcgcg                                                                                                                                                    >  1:260945/1‑156 (MQ=255)                                                                                                                                                                                   cGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCACTATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGCTTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGGGat                                                   >  2:289724/1‑251 (MQ=255)                                                                                                                                                                                   cGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCCGCGgcgc                                                                                                                                                     >  2:328874/1‑153 (MQ=255)                                                                                                                                                                                   cGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGgcgc                                                                                                                                                     <  1:328874/153‑1 (MQ=255)                                                                                                                                                                                                 gatgtGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAatat                                     <  1:99606/247‑1 (MQ=255)                                                                                                                                                                                                  aTTTGT‑AAATTCGCTCCGCGTAATAAGCTGATTGGGATCTCCCTGTTCGCGGTTTTTTGGTCAAGAAGAGTGGAAATTGGCGTTTAACGATTATGTTGGGCGCGCTCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAAGGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGAGAATATTGATAATAtt                                    <  2:324651/251‑1 (MQ=255)                                                                                                                                                                                                      gT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGGGATATTATTGATAATAttca                                  >  2:198596/1‑247 (MQ=255)                                                                                                                                                                                                                                gACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCACTATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGCACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCACCCCCCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGtt       >  2:214135/1‑251 (MQ=255)                                                                                                                                                                                                                                      gATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACg  >  2:403231/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  AATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACG  >  NC_000913/4640672‑4641149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑