Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,640,864	A→G	intergenic (+93/‑78)	ytjE → / → yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,640,864	0	A	G	100.0%	111.7 / NA	34	intergenic (+93/‑78)	ytjE/yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

AAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGAT  >  NC_000913/4640642‑4641100                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              aaaTGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAg                                                                                                                                                                                                                   <  1:364630/250‑1 (MQ=255)        aGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAg                                                                                                                                                                                                            <  2:111596/250‑1 (MQ=255)                               aatCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTAttt                                                                                                                                                                                    >  1:283218/1‑251 (MQ=255)                                      cTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCa                                                                                                                                                                               >  1:77121/1‑249 (MQ=255)                                              atcatACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTgg                                                                                                                                                                     >  1:371853/1‑251 (MQ=255)                                               tcatACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCCACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTACGCTAAGCAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGc                                                                                                                                                                    >  2:453603/1‑251 (MQ=255)                                                      aCTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGc                                                                                                                                                             <  1:262407/251‑1 (MQ=255)                                                            aaaCGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATaa                                                                                                                                                       <  2:77121/251‑1 (MQ=255)                                                             aaCGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAAc                                                                                                                                                      <  1:31616/251‑1 (MQ=255)                                                                 tggtgAATCCAATTGTCGAGATTTATTTTTTATAAAATTAACCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTCCAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTATAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGatt                                                                                                                                                  >  1:422689/1‑251 (MQ=255)                                                                                 cGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCg                                                                                                                                  >  1:437644/1‑251 (MQ=255)                                                                                                  aaaaTTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATAtt                                                                                                                 >  2:99606/1‑251 (MQ=255)                                                                                                       tATCCTAAGGAAACAGAAGGATATATGGCATTATTTAACAATACAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTGAGGATT‑GTGAAATTCGCACCGCGAAATAAGTAGACAGTGATCACCCTTTTCGCGGTTATTTGTTCAAGAAGAGTGGCAAAATGCGTAGAAGGATTATTCTGGGCTCACCCGCCAGAGCAGAAAATATTgggg                                                                                                             <  2:246658/250‑1 (MQ=255)                                                                                                           cTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCACCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAgcg                                                                                                        >  2:397936/1‑251 (MQ=255)                                                                                                                   aCAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGc                                                                                                <  1:466974/251‑1 (MQ=255)                                                                                                                    cAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGtaataa                                                                                                                                                                                                                    >  2:92869/1‑134 (MQ=255)                                                                                                                    cAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGtaataa                                                                                                                                                                                                                    <  1:92869/134‑1 (MQ=255)                                                                                                                           atttGTAGCATTTTTGAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTCCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCCCCGCGTAATAAGTTGACATTGATCACCCTGGTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTTTAACGATTATTCTGGTCGCGCCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGaaaa                                                                                        <  2:39995/248‑1 (MQ=255)                                                                                                                                           aacaatTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgggg                                                                                                             >  1:56576/1‑214 (MQ=255)                                                                                                                                           aacaatTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTGGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgggg                                                                                                             <  2:56576/214‑1 (MQ=255)                                                                                                                                                  cAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCgg                                                                 <  1:303594/251‑1 (MQ=255)                                                                                                                                                    aCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGAt                                                               >  1:19796/1‑251 (MQ=255)                                                                                                                                                                 tCAGGAACTAATTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTCAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGCCAGTGATCACCCTGTTCGCGGATATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGACACTATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAgac                                                    >  2:438371/1‑247 (MQ=255)                                                                                                                                                                             ttAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTAAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGa                                       <  2:371853/250‑1 (MQ=255)                                                                                                                                                                                gCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGagcc                                    >  2:150182/1‑250 (MQ=255)                                                                                                                                                                                 ccTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCCCCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGagccag                                  <  1:150182/251‑1 (MQ=255)                                                                                                                                                                                     ttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGTGGCAGCGGCGCGGGCACTGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAggaccccc                                           >  2:399484/1‑232 (MQ=255)                                                                                                                                                                                     ttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACAcc                                           <  1:399484/238‑1 (MQ=255)                                                                                                                                                                                                gTAAAGGGCTTTTTCTGAGCCTTCCGATAAGGATTTGC‑GAATTCGCACCGCGTAATAAGTCGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAACATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGGAAATATTGGGGCAGCGGCGCGGGCGATGAAAACGATGGGTTATCGCGTACTGCGGATTGTCGTTAGCCAGGCACACCTGGAGCAAGCCACCCGCTggggcg                   >  2:82576/1‑248 (MQ=255)                                                                                                                                                                                                               tGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGcgcg                                                                                                   >  1:260945/1‑156 (MQ=255)                                                                                                                                                                                                               tGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGcgcg                                                                                                   <  2:260945/156‑1 (MQ=255)                                                                                                                                                                                                                 cGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCACTATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGCTTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGGGat  >  2:289724/1‑251 (MQ=255)                                                                                                                                                                                                                 cGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCCGCGgcgc                                                                                                    >  2:328874/1‑153 (MQ=255)                                                                                                                                                                                                                 cGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGgcgc                                                                                                    <  1:328874/153‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              AAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGAT  >  NC_000913/4640642‑4641100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑