Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,640,785	C→T	intergenic (+14/‑157)	ytjE → / → yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,640,785	0	C	T	100.0%	79.0 / NA	25	intergenic (+14/‑157)	ytjE/yjtD	protein YtjE/putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

GACACCCAATAAAACTACGCTTCGTTGACATATATCAAGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAA  >  NC_000913/4640560‑4641014                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       gACACCCAATAAAACTACGCTTCGTTGACATATATCAAGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAAt                                                                                                                                                                                                               >  1:247889/1‑251 (MQ=255)              aCTACGCTTCGTTGACATATATCAAGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCtttt                                                                                                                                                                                                  >  1:17278/1‑251 (MQ=255)                  cGCTTCGTTGACATATATCAAGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATGTTTTAACAATTCAACCGTTAGTACAGTCAGGAAAGAGTTTAGCCTTTTTTaa                                                                                                                                                                                              <  2:338689/251‑1 (MQ=255)                        gTTGACATATATCAAGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAg                                                                                                                                                                                        <  2:475693/251‑1 (MQ=255)                            aCATATATCAAGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTaaa                                                                                                                                                                                    <  2:247889/251‑1 (MQ=255)                                     aaGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCttttt                                                                                                                                                                           <  2:407751/251‑1 (MQ=255)                                                    aCGTTAACGGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATCATCAAACTAGTTGAATCATACAACTAATAAACGTGGAGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAACTAGTTTAGCCTTTTTTAAGCTAAGTAACGGGCTTTTTCTGCGACTTACGtt                                                                                                                                                             >  1:301738/1‑250 (MQ=255)                                                                                   aaaTGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAg                                                                                                                             <  1:364630/250‑1 (MQ=255)                                                                                          aGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAg                                                                                                                      <  2:111596/250‑1 (MQ=255)                                                                                                                 aatCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTAttt                                                                                              >  1:283218/1‑251 (MQ=255)                                                                                                                        cTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCa                                                                                         >  1:77121/1‑249 (MQ=255)                                                                                                                                atcatACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTgg                                                                               >  1:371853/1‑251 (MQ=255)                                                                                                                                 tcatACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCCACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTACGCTAAGCAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGc                                                                              >  2:453603/1‑251 (MQ=255)                                                                                                                                        aCTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGc                                                                       <  1:262407/251‑1 (MQ=255)                                                                                                                                              aaaCGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATaa                                                                 <  2:77121/251‑1 (MQ=255)                                                                                                                                               aaCGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAAc                                                                <  1:31616/251‑1 (MQ=255)                                                                                                                                                   tggtgAATCCAATTGTCGAGATTTATTTTTTATAAAATTAACCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTCCAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTATAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGatt                                                            >  1:422689/1‑251 (MQ=255)                                                                                                                                                                   cGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCg                                            >  1:437644/1‑251 (MQ=255)                                                                                                                                                                                    aaaaTTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATAtt                           >  2:99606/1‑251 (MQ=255)                                                                                                                                                                                         tATCCTAAGGAAACAGAAGGATATATGGCATTATTTAACAATACAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTGAGGATT‑GTGAAATTCGCACCGCGAAATAAGTAGACAGTGATCACCCTTTTCGCGGTTATTTGTTCAAGAAGAGTGGCAAAATGCGTAGAAGGATTATTCTGGGCTCACCCGCCAGAGCAGAAAATATTgggg                       <  2:246658/250‑1 (MQ=255)                                                                                                                                                                                             cTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAATTCACCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAgcg                  >  2:397936/1‑251 (MQ=255)                                                                                                                                                                                                     aCAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGc          <  1:466974/251‑1 (MQ=255)                                                                                                                                                                                                      cAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGtaataa                                                                                                                              <  1:92869/134‑1 (MQ=255)                                                                                                                                                                                                      cAGAAGGATATGTAGCATTTTTTAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGtaataa                                                                                                                              >  2:92869/1‑134 (MQ=255)                                                                                                                                                                                                             atttGTAGCATTTTTGAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTCCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCCCCGCGTAATAAGTTGACATTGATCACCCTGGTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTTTAACGATTATTCTGGTCGCGCCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGaaaa  <  2:39995/248‑1 (MQ=255)                                                                                                                                                                                                                             aacaatTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgggg                       >  1:56576/1‑214 (MQ=255)                                                                                                                                                                                                                             aacaatTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTGGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgggg                       <  2:56576/214‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       GACACCCAATAAAACTACGCTTCGTTGACATATATCAAGTTCAATTGTAGCACGTTAACAGTTTGATGAAATCATCGTATCTAAATGCTAGCTTTCGTCACATTATTTTAATAATCCAACTAGTTGCATCATACAACTAATAAACGTGGTGAATCCAATTGTCGAGATTTATTTTTTATAAAATTATCCTAAGTAAACAGAAGGATATGTAGCATTTTTTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGT‑AAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAA  >  NC_000913/4640560‑4641014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑