Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,440	C→T	T421T (ACC→ACT)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,440	0	C	T	89.7%	68.6 / ‑0.6	29	T421T (ACC→ACT)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (13/13);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

GCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGC  >  NC_000913/4639197‑4639690                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           gCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAata                                                                                                                                                                                                                                                     <  1:385989/251‑1 (MQ=255)                                   gCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGAATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGaa                                                                                                                                                                                                                   <  1:271717/251‑1 (MQ=255)                                       ggATTATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCTGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAg                                                                                                                                                                                                                 <  1:261412/249‑1 (MQ=255)                                        gATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGCAAATGAAAGcc                                                                                                                                                                                                               >  1:189924/1‑250 (MQ=255)                                                 cAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGGAAATGAAAGCCAGTAaaga                                                                                                                                                                                                       >  1:455006/1‑249 (MQ=255)                                                     cTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAACATGTTGTTTTCCCTCGCGGTGTTGTTTCTGGGTGG‑GTGGATGGGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTTTGCTGGGGGTGGCGCTGGCCGGCCTTATGGTTGTGACTCGTATAATCGACTGGGATGGGTTTTCCATTCCGGAAATGAAAACCaaaaaaaaaattt                                                                                                                                                                                                  >  2:350759/1‑238 (MQ=255)                                                            gggCGTTAATTAACGGTATTTAATTGCAGGCGGTATTGAAAGGCTGGAGCAACAGCATGGTGTTTACCTTCGCGCGTTTGTTGCTGGATGGTGTG‑ATGTGGGGACGGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGTGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGAc                                                                                                                                                                                          <  1:388531/251‑1 (MQ=255)                                                                gttAATGAACGTTTTTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGagc                                                                                                                                                                                      <  2:345576/249‑3 (MQ=255)                                                                      gcaCGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGCTGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGGGTTAc                                                                                                                                                                                >  2:228652/3‑251 (MQ=255)                                                                                 atttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGaaa                                                                                                                                                                     <  1:228652/251‑1 (MQ=255)                                                                                       aggCGGTATTGAAAGGCTGGCGCAAAAGCATGAAGTTTAAACTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGAATCTGGAAATAAGGt                                                                                                                                                               >  1:406190/3‑251 (MQ=255)                                                                                        ggCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGAGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCCCTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGtt                                                                                                                                                              >  2:202259/2‑251 (MQ=255)                                                                                           ggTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGaa                                                                                                                                                           <  1:247643/251‑1 (MQ=255)                                                                                             tATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACACTGGACGATCAATTACGTATCTGGAAATAAGGTTGaaa                                                                                                                                                          >  1:124786/1‑250 (MQ=255)                                                                                                 gAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaa                                                                                                                                                     <  1:236208/251‑1 (MQ=255)                                                                                                   aaGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGCGTG‑ATGTGGGGACTGCTCAACTCTGACGATAGCGCGCTGATGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaaa                                                                                                                                                    >  1:456014/1‑250 (MQ=255)                                                                                                            gCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCt                                                                                                                                           >  2:82751/1‑250 (MQ=255)                                                                                                             cAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTa                                                                                                                                          >  1:25784/1‑250 (MQ=255)                                                                                                                   aaggttgttTACCCTCTCGCTGTGGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAgcg                                                                                                                                   <  2:174032/248‑1 (MQ=255)                                                                                                                     tgttgtTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAgc                                                                                                                                    <  1:244766/248‑1 (MQ=255)                                                                                                                     tgttgtTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAgc                                                                                                                                    >  2:244766/1‑248 (MQ=255)                                                                                                                                                       tgtg‑ATGTGGGTACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTACAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCtt                                                                                               >  2:428747/1‑251 (MQ=255)                                                                                                                                                       tgtg‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCtt                                                                                               >  1:115755/1‑251 (MQ=255)                                                                                                                                                            aTGTGGGGACTGCTAAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAg                                                                                           <  2:115755/251‑1 (MQ=255)                                                                                                                                                                       gCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGa                                                                                <  1:82751/251‑1 (MQ=255)                                                                                                                                                                          cAACTCTGCCGATAGCGCGCTGGTGGTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGAACAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGc                                                                             <  1:733/251‑1 (MQ=255)                                                                                                                                                                                                         aCCAGTGTGCTGGCGTTTGCGCTGGCCGGCATGATTTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTTCCGAAAAGGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTTAAAAATAAAAACGTCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGTGATAACCTTCACCGTGGATGGTGGCgatga                                               <  1:302611/250‑1 (MQ=255)                                                                                                                                                                                                                           gcgcTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTtccggcgtatccggcgt                            >  1:307787/1‑251 (MQ=255)                                                                                                                                                                                                                                          atgTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAA‑CGGCGCTAAAAAGAGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGtttt            >  2:307759/1‑250 (MQ=255)                                                                                                                                                                                                                                                     ccGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAATTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcggc  <  2:189924/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           GCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGC  >  NC_000913/4639197‑4639690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑