Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,299	T→C	G374G (GGT→GGC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,299	0	T	C	92.5%	92.3 / 0.6	41	G374G (GGT→GGC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (16/21);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-02

TGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAA  >  NC_000913/4639053‑4639544                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ggACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCGCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGGCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTggc                                                                                                                                                                                                                                                    <  1:40254/250‑2 (MQ=255)       ggCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAAc                                                                                                                                                                                                                                               >  1:162473/1‑250 (MQ=255)                gTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCAtgttgt                                                                                                                                                                                                                                     <  1:378909/251‑1 (MQ=255)                                   gCACTGACTTTTAGGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACTTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTgttgtt                                                                                                                                                                                                                  <  2:202596/251‑1 (MQ=255)                                            tttATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGGAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTTTTGCTGGGTgg                                                                                                                                                                                                         >  1:95570/1‑251 (MQ=255)                                              tATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGgtggtg                                                                                                                                                                                                       >  1:158772/1‑251 (MQ=255)                                                    gTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑Atgt                                                                                                                                                                                                <  1:146644/251‑1 (MQ=255)                                                        tttGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTTGTTACCCTCCCGCTGTTGTTGCttggtggggtcggaagggg                                                                                                                                                                                             >  2:379679/1‑233 (MQ=255)                                                        tttGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATg                                                                                                                                                                                                          <  1:379679/238‑1 (MQ=255)                                                           tgttttGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTTGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCTCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGAc                                                                                                                                                                                          <  1:469704/248‑1 (MQ=255)                                                               ttGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCt                                                                                                                                                                                      <  2:162473/250‑1 (MQ=255)                                                                                cAACGTTTACAACCAATGCCATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTTAAAGGATGGCGCCACAGCATGTTTTTTATCCCCGCGCTGTTGTTGCTGGGTGGGGTG‑GTGGGGGGACTGGTCAACTCCGCCGCTAgggg                                                                                                                                                                    >  2:313745/1‑248 (MQ=255)                                                                                 aaCGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGGGTG‑ATGTGGGGACTGCCTAACTCTGCCGATAgcgcg                                                                                                                                                                   >  1:396318/1‑251 (MQ=255)                                                                                                  cAATATTTGCGGGTGGTGCTTTCATTGGGGATGTTTTATTTGTTCTTGCTGGAGCTTTTTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAg                                                                                                                                                  <  2:150170/251‑1 (MQ=255)                                                                                                     tatTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGGAGGGGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgtgt                                                                                                                                               >  1:34198/1‑251 (MQ=255)                                                                                                                ggggTTTTCATGGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGTTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtggtggtgg                                                                                                                                    <  2:65294/251‑1 (MQ=255)                                                                                                                          ttGGTGCTGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCATCGCGCTGTTTTTTCTGGGTGGTGTG‑ATGTTGGGACTGCTAAACTCTGCCGTTATCGGGCTGGTGTTGGGGACCAGTGTCGtggtgggtgggcctgccgg                                                                                                                          >  2:388531/1‑238 (MQ=255)                                                                                                                                  gTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGt                                                                                                                  <  1:471047/251‑1 (MQ=255)                                                                                                                                     ttATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGttt                                                                                                                <  1:313745/250‑1 (MQ=255)                                                                                                                                                 gCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAata                                                                                                   <  1:385989/251‑1 (MQ=255)                                                                                                                                                       gcTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCCACAGCATGTTGTTTACACTCGCGCTGATGTTGCTGGATGGTGTG‑ATGTGGGGGCTGCTAAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtg                                                                                                                                           >  1:286883/1‑205 (MQ=255)                                                                                                                                                       gcTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCtg                                                                                                                                           <  2:286883/205‑1 (MQ=255)                                                                                                                                                          ttCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCa                                                                                                                                                                                                                                           >  2:151693/1‑107 (MQ=255)                                                                                                                                                          ttCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCa                                                                                                                                                                                                                                           <  1:151693/107‑1 (MQ=255)                                                                                                                                                                                   gCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTTTTGCTGGGTGGTGTG‑Atg                                                                                                                                                                                                 >  2:273212/1‑123 (MQ=255)                                                                                                                                                                                   gCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑Atg                                                                                                                                                                                                 <  1:273212/123‑1 (MQ=255)                                                                                                                                                                                   gCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGAATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGaa                                                                 <  1:271717/251‑1 (MQ=255)                                                                                                                                                                                       ggATTATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCTGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAg                                                               <  1:261412/249‑1 (MQ=255)                                                                                                                                                                                        gATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGCAAATGAAAGcc                                                             >  1:189924/1‑250 (MQ=255)                                                                                                                                                                                                 cAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGGAAATGAAAGCCAGTAaaga                                                     >  1:455006/1‑249 (MQ=255)                                                                                                                                                                                                     cTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAACATGTTGTTTTCCCTCGCGGTGTTGTTTCTGGGTGG‑GTGGATGGGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTTTGCTGGGGGTGGCGCTGGCCGGCCTTATGGTTGTGACTCGTATAATCGACTGGGATGGGTTTTCCATTCCGGAAATGAAAACCaaaaaaaaaattt                                                >  2:350759/1‑238 (MQ=255)                                                                                                                                                                                                            gggCGTTAATTAACGGTATTTAATTGCAGGCGGTATTGAAAGGCTGGAGCAACAGCATGGTGTTTACCTTCGCGCGTTTGTTGCTGGATGGTGTG‑ATGTGGGGACGGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGTGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGAc                                        <  1:388531/251‑1 (MQ=255)                                                                                                                                                                                                                gttAATGAACGTTTTTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGagc                                    <  2:345576/249‑3 (MQ=255)                                                                                                                                                                                                                      gcaCGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGCTGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGGGTTAc                              >  2:228652/3‑251 (MQ=255)                                                                                                                                                                                                                                 atttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGaaa                   <  1:228652/251‑1 (MQ=255)                                                                                                                                                                                                                                       aggCGGTATTGAAAGGCTGGCGCAAAAGCATGAAGTTTAAACTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGAATCTGGAAATAAGGt             >  1:406190/3‑251 (MQ=255)                                                                                                                                                                                                                                        ggCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGAGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCCCTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGtt            >  2:202259/2‑251 (MQ=255)                                                                                                                                                                                                                                           ggTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGaa         <  1:247643/251‑1 (MQ=255)                                                                                                                                                                                                                                             tATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACACTGGACGATCAATTACGTATCTGGAAATAAGGTTGaaa        >  1:124786/1‑250 (MQ=255)                                                                                                                                                                                                                                                 gAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaa   <  1:236208/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aaGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGCGTG‑ATGTGGGGACTGCTCAACTCTGACGATAGCGCGCTGATGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaaa  >  1:456014/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTG‑ATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAA  >  NC_000913/4639053‑4639544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑