Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,053	T→G	T292T (ACT→ACG)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,053	0	T	G	100.0%	142.2 / NA	47	T292T (ACT→ACG)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/23);  total (24/23)

GGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAG  >  NC_000913/4638810‑4639297                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ggtgCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCCTCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCACAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGAGTCGCCGTGACGAACCCAGCCGATCAATACCAATTATCGGACCggg                                                                                                                                                                                                                                                >  2:276645/1‑251 (MQ=255)  gtgcgtgaggggctTAAGAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTGGCCAGCCAAACGGGAAGTTGGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGGGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGc                                                                                                                                                                                                                                               <  2:190724/242‑1 (MQ=255)            ggcgTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTAc                                                                                                                                                                                                                                     <  1:466985/251‑1 (MQ=255)                    aGCGAAATGACCTTAACCAGCAACTGGCCGCAGCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTct                                                                                                                                                                                                                             <  2:187248/251‑1 (MQ=255)                               cTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGATTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGCCGCCAGCCGATCAATACCAATTAACGGACCg                                                                                                                                                                                                                                                  >  1:207134/1‑219 (MQ=255)                                 tAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAATTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCAc                                                                                                                                                                                                                  <  1:28315/249‑1 (MQ=255)                                                    tCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCCGGGACGACGCCAGCCGGTCAATACCACTTAACGGACCGGGGGACTAAGTACGCCCTTCCTCTGGTTGCACTGACTTTTATGGCGtgttttt                                                                                                                                                                                             >  2:247643/1‑245 (MQ=255)                                                              ttAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGAACAATACCAATTAACGGACCGGGCGACTAAGGACGCCATTCTGCTGGTTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAAc                                                                                                                                                                                   >  2:109615/1‑251 (MQ=255)                                                                        ttttgccAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAAGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAAAGCTCACcgtc                                                                                                                                                                         >  1:107003/6‑249 (MQ=255)                                                                                    aaCGGGAAGTTAGCGAGTCATGTTTTCAGGCGCAAGGGCAAAGCAGCTGGTTTGCGAATAATCGCGGTGAGCGGTTTGCGTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTGTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATGAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTacac                                                                                                                                                             <  1:449691/251‑1 (MQ=255)                                                                                     aCGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACAcc                                                                                                                                                            <  1:238391/251‑1 (MQ=255)                                                                                      cGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACAccc                                                                                                                                                           >  1:163409/1‑251 (MQ=255)                                                                                       gggAAGTTAGCGAGTCAGGTTTTCAGGCGAAATGGCAAAGCAGCTGGTTTGATAATAATCTCGGTGAGCGTTTTGATTCAGGCAATGATACAGGCTGGGAAAAATTCACCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGAGCAATACCAATTACAGGACCGGGCGGCTAAGTACGCCATTCTGCTGAATGCACTGAATTTTATGACGTACTTTGTTTTTGAAAAGCTCAACGCACAACGTTTACAcca                                                                                                                                                           >  1:316431/1‑249 (MQ=255)                                                                                            gTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCCATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCCCCGCGCAACGTTTACACCCCATGcc                                                                                                                                                     >  2:248691/1‑250 (MQ=255)                                                                                              tAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGCTCAATTCCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGTTTGCACTGCCTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAat                                                                                                                                                   >  2:111212/1‑251 (MQ=255)                                                                                              tAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCaa                                                                                                                                                    <  1:248691/250‑1 (MQ=255)                                                                                               aGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAata                                                                                                                                                  <  1:305282/251‑1 (MQ=255)                                                                                                  gatgccggTTTTAAGGCGCATTGGCAAAGCATGGGGTTGGCTTAAAATCTCGGTGAGCGTGGTGCTTCAGGCAAGGATGCCGGGTGGGAAAACTCCCCCGCGTTTAGCGTCGCATGGACGACGCCAGCCGATCAAGACCAATTAACGGACCGTGCGACTAAGGACGATATTCTGCTGATTGCACGGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACTCCCAATGCAatagtt                                                                                                                                               <  2:183988/245‑4 (MQ=255)                                                                                                      cAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCATTGCAATATTTGCtg                                                                                                                                           >  2:125983/1‑251 (MQ=255)                                                                                                        ggTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAAAGCTCACCGCGCAACGTTTACACCCAATGCAATATTTTCtggg                                                                                                                                         >  2:378909/1‑250 (MQ=255)                                                                                                          tcttCAGGCGCACTGGCAAAGCAACTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAGTGATCCCGTCTGGAAAAACTTCCCCGCGTTTAGCGGCGCAGTGACGACCCCCGCCGATCAATAACCATTAACGGGCCGGGCGAATAAGTAAGCCATTCTGCTGGTTGCCCTGACTTTTATGGCGGTCTTTGTTTTTGCAAAGCTCCCCACACACCGTTTACACCCACGGCAATATTTTCtggtgg                                                                                                                                       >  2:302611/3‑251 (MQ=255)                                                                                                                         gCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAAc                                                                                                                                                                     >  1:70752/1‑206 (MQ=255)                                                                                                                         gCAAAGCAGCTGGTTTGATAATAATCCCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAAc                                                                                                                                                                     <  2:70752/206‑1 (MQ=255)                                                                                                                           aaaGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGGGACGAAGCCAGCCGATCAATACCCATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGTTTGCACTGCCTTTTATGACGTTCTTTGGTTTTGAAACGCTCCACGCGCATAGTTTACACCCCCTGTAGTCATTGCTGGTGGGGATTTCATTggggcat                                                                                                                      >  2:733/1‑246 (MQ=255)                                                                                                                                  cTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACCCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGCGAT‑Gttttttt                                                                                                              >  2:376050/1‑248 (MQ=255)                                                                                                                                    ggTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCCCCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGGGAT‑GTTTTATTTg                                                                                                            >  2:385989/1‑251 (MQ=255)                                                                                                                                      tttGCTAATAATCTCGGTGATCGTTTTGCTTAAGGCAATGTTACCGGCTGGGAAAACTTCCCCGCGTTTATCGGCTCAGTGACGACTCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTTCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATGTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGc                                                                                                           <  2:459127/250‑1 (MQ=255)                                                                                                                                      tttGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGAATGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCCATATTTGCTGGGGGGGATTTCATTGGGGATTGTTTT‑TTTGct                                                                                                          >  2:471047/1‑251 (MQ=255)                                                                                                                                         gCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCGGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGctct                                                                                                        <  2:133780/250‑1 (MQ=255)                                                                                                                                            aataatCTCGGTGAGCTTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCt                                                                                                    <  1:266832/251‑1 (MQ=255)                                                                                                                                             ataatCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCTGCTGGGAAAACTTCCACGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCAGTGGTGAT‑GTTTTATTTGCTCTTGCTg                                                                                                   <  1:78344/251‑1 (MQ=255)                                                                                                                                                       tGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTg                                                                                         >  1:11644/1‑251 (MQ=255)                                                                                                                                                                 gCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCgg                                                                               <  2:163409/251‑1 (MQ=255)                                                                                                                                                                                aCCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCAt                                                                 <  1:109615/250‑1 (MQ=255)                                                                                                                                                                                   ggtggggAAAACTCCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTCGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGAt                                                             <  2:360391/247‑1 (MQ=255)                                                                                                                                                                                   ggCTGGGAAACCTCCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATTCCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGAt                                                             <  2:243655/251‑1 (MQ=255)                                                                                                                                                                                       gggAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATaa                                                           <  1:376050/249‑1 (MQ=255)                                                                                                                                                                                             aCTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGCACCTATCGGTTTTACCGTGGCCTGGATAATCGCCAGt                                                   >  2:40254/1‑251 (MQ=255)                                                                                                                                                                                                                 gCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGa                                >  1:345576/1‑250 (MQ=255)                                                                                                                                                                                                                    gtgacgacgCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACgg                            >  2:236208/4‑251 (MQ=255)                                                                                                                                                                                                                            gCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtatttatt                    >  1:174032/1‑251 (MQ=255)                                                                                                                                                                                                                            cccAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtatttatt                    <  2:11644/250‑1 (MQ=255)                                                                                                                                                                                                                              cAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTg                  >  1:65294/1‑251 (MQ=255)                                                                                                                                                                                                                               aGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGc                 <  1:111212/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaTTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGaaa   <  1:276645/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAg  <  1:125983/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGAATTgga    >  1:13888/1‑247 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAT‑GTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAG  >  NC_000913/4638810‑4639297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑