| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 7,474 | T→C | Q162Q (CAA→CAG) | yaaJ ← | putative transporter YaaJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 7,474 | 0 | T | C | 96.8% | 85.8 / ‑4.7 | 31 | Q162Q (CAA→CAG) | yaaJ | putative transporter YaaJ |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (17/13); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
AAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAA > NC_000913/7248‑7709 | aaaTAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGCAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGc > 2:245681/1‑249 (MQ=255) gACCAAATGACGTGGGGAGGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGCCGCAATGAAGACCGCGAGTGATTGCCAGCAGAGAAAAGACAGCGAGACTAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCACGGGACACGGCGTACGCTTAACCTCAGCTGAAAATTCAGCCATAGGc > 2:15719/1‑251 (MQ=255) gACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGc > 1:54308/1‑251 (MQ=255) cAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCCACAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGCGTGATCGCCAGCAGAGCAATGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCACAAGAAAAACTCAGGGCTCGCGCAACGGCGTCCGCCTGCCTTCCGCTGTAAATTATGCCGTAGGCGAt > 2:123262/1‑251 (MQ=255) aggggAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCaaaa > 2:95815/2‑250 (MQ=255) ttCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCCGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGACAAGAACGcc > 2:362665/1‑251 (MQ=255) cGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGCACGCCCATcc > 1:340463/1‑249 (MQ=255) cAGGATGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCa < 2:54308/251‑1 (MQ=255) cTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCAGCTGAAAATTATGCCGTAGGCGATGAGCAAAACGACGGCGAACAGAACGCCCATCCAGCGCATCCCCCGccc > 2:486054/1‑251 (MQ=255) tATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGTCGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACTGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATg > 2:347145/1‑251 (MQ=255) cGCCAGCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGTGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCg < 1:247740/251‑1 (MQ=255) cATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTc > 1:119920/1‑251 (MQ=255) aaCGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGcc < 1:310811/251‑1 (MQ=255) aaccccTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTg < 1:486054/248‑1 (MQ=255) gCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAGAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCCTATACCATGCAGGTCCGCCACGAAACTGCCCAttt > 1:312945/1‑250 (MQ=255) atgggCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTggc < 2:340463/247‑3 (MQ=255) gagcaaCGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAACACAGCGAGACTAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAGCTCAGGGATCGCGCAACGACATTCGCCTGCATTCCGCTGAAAATTATGCCGTAGGCGATGACCCAGAAGACGGCGAGCCGACCGCCCATCCACCGCATCCCCAGCCCGCGCGCCATATACAATGACGTTCCGCCACGaaaatgacacatgacctcaagt > 2:176615/6‑232 (MQ=255) aGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACTGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAgctg < 1:347145/251‑4 (MQ=255) aCCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGcc < 1:413341/202‑1 (MQ=255) aCCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGcc > 2:413341/1‑202 (MQ=255) gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGGCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgagggaa < 1:239638/246‑8 (MQ=255) cagcagAGCAAAGACAGCGAGAATAATACCTGTCCCCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCgg < 2:331976/251‑1 (MQ=255) agcagAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGCAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGc > 2:92594/1‑251 (MQ=255) cagAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGCAAATCAAAAGAAACACTCAGGGCGCGCGCAACGGCGTTCGCCTGAATTCCGCTGCAAATTATGCCGTAGGCGATGCGCAAAAAGCCGACGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCGTCTACCATGCCGGTCCGCCACGCAACTGCCCATTGGCATCACGCTCTTTCTaaatctgtgcgaggaaacattctgac > 2:211637/1‑227 (MQ=255) aaaGACAGCTAGAATAATACCTGTCACCCGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGTCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAgg < 2:204770/251‑1 (MQ=255) cGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAACACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCAATg > 2:190682/1‑251 (MQ=255) gaaaaataccTGCCACCCGCGGGGGAAAAGCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTGCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCg < 2:3472/243‑1 (MQ=255) gaATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCg > 1:271435/1‑251 (MQ=255) aaaGAAAAACTCAGGGCTCGCGCAACGGCGTTCGTCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCAAATACCAAGCCGTTACGCCACGAAACTGCCCATTGGCATCACGTTCATTATAAAGCTGAGCGAGGGAAAATTCGGCAACCGCGGTAGCCATGCCGGTAAACGCGGAAAccccacaccagaaacc > 2:220542/1‑235 (MQ=255) aGGGCGCGCGAAACGGCGTGCGTCTGAATTCTGCTGAAAATTAGGCCGTAGGCGATGAGAAAAAAGCCGGCAAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATATCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCAcc < 2:93977/251‑1 (MQ=255) cgaaaCGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTaa < 2:411608/248‑1 (MQ=255) | AAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAA > NC_000913/7248‑7709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |