Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,587	C→T	T137I (ACC→ATC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,587	0	C	T	100.0%	159.1 / NA	50	T137I (ACC→ATC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/22);  total (28/22)

GTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCG  >  NC_000913/4638343‑4638832                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTAAACGCGCCAAATATCATCTTAgg                                                                                                                                                                                                                                                  >  2:41693/1‑251 (MQ=255)          cttaTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGccctt                                                                                                                                                                                                                                         >  2:270342/4‑248 (MQ=255)                      ccTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGA‑GAACGCAAGATAGGGATTTATACCTGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGc                                                                                                                                                                                                                            <  1:171384/251‑1 (MQ=255)                          tgACCGAGCTTTATACGGTGCAGGAAGAGGATACAACCGTGGAGCGGAAACGAAGTTTTCTCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAGTCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCAATTTCGAGGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGCCAAGCCCTTTATTGTGATTAGCGTc                                                                                                                                                                                                                         >  1:435018/1‑251 (MQ=255)                                 gCTTTATACTGTGAAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCGTTATTGTGATTAGCGTAGGGGATg                                                                                                                                                                                                                  <  2:31723/251‑1 (MQ=255)                                          ggTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGCAGTTTTATCCATTTTTGGTTACCTGAGTCATTGCTGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGCTGCGCG‑GGGt                                                                                                                                                                                                         >  2:466985/1‑250 (MQ=255)                                            ggCAGTAAGAGGATAAAGCGGTGGATCGGAAACGAAGTTGTATCCAGTTTTGGTTAGCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGAAAGATAGGGATTTATCCCGGTCAGGTCTGGCACAGGGATTTAACGCTAAAAGCTGATTTCGATGTTTCGCGTCGTAGCGAGCTCAACGCGGCAAATAGCATCTTAGGCAATCCCTTTATTGTGATTAGCGTCGGGGAGGCGCT‑TGGTagt                                                                                                                                                                                                      <  2:412619/250‑3 (MQ=255)                                              cAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATtgg                                                                                                                                                                                                    >  1:122133/1‑251 (MQ=255)                                                ggAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGCGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATtggtg                                                                                                                                                                                                  >  2:3361/1‑251 (MQ=255)                                                     agGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATtggtgtggtg                                                                                                                                                                                             <  1:362077/251‑1 (MQ=255)                                                           aaaCCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAgcg                                                                                                                                                                                       <  1:339553/251‑1 (MQ=255)                                                            aaCCGTGGAGCGGTAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGATGAAGGCAATCAGAACGTAGCAGAACGCAAGATAGGGATTTATAACGGTCAGGTCTGGCAAAGTGATTTACCGCTAAAAGCCAATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATAATCTTAGTCAAGACCTTTTTTGTGATTAGCGTCGGCGATGCTCG‑TGATTTTGGTGAGGAGAATgcgc                                                                                                                                                                                      >  2:266832/1‑251 (MQ=255)                                                                       ggAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGGGTGGTGGAAGGGCCTggaggtaa                                                                                                                                                                            >  2:305282/1‑243 (MQ=255)                                                                        gAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGAt                                                                                                                                                                                                                   <  1:212420/211‑1 (MQ=255)                                                                        gAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGAt                                                                                                                                                                                                                   >  2:212420/1‑211 (MQ=255)                                                                          aaggAAGTTTTATCCAATTTTGGTTACATGAGTCATTGATGGTTGATGGCAATCAGAACGTGGTAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTGACGCTAAAAGCCGATTTCGATGTTTCGCGTCTAAGCGAGCTCAACGCGCCAACTATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGAGTTGGGGTGGTGAAGGCGCCTGCAGTTaacgg                                                                                                                                                                         >  2:28315/4‑250 (MQ=255)                                                                           acggaGTTTTATACATTTTTGGTTACCGGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGCACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCAAAGTAATTTAAAGATAAAGGCCTATTTCGATGTTTAGTGTCTAAGAGTGCTCAACGCGACAAATATCATCTTAGGAAAGACCTTTATTGTGCTTAGAGTCGGGGGTGCGCGGTGG‑ATTGGTGAGGTAAAAgagacgagatttagctga                                                                                                                                                                        >  2:261412/5‑233 (MQ=255)                                                                             gAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTaacggaacg                                                                                                                                                                     <  1:332330/251‑1 (MQ=255)                                                                                   ttATCAATTTTTGGTTACATGAGTCATTGATGGTTGATGGCAATAAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGGAAGAGCCTGAAGTTACCGGAACGGCGCTg                                                                                                                                                               >  2:238391/1‑251 (MQ=255)                                                                                    tATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTgg                                                                                                                                                              >  1:183988/1‑250 (MQ=255)                                                                                            tttGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGAGTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGa                                                                                                                                                       <  2:452112/250‑1 (MQ=255)                                                                                                 tcaCCTGAGACATTGATGGTTGATGGCAATCAGAACGTGGTATAACGAAAGATAGGGATTTATACCGGTCAGGTCTGTCACAGTGGTTTAAAGATTAAAGACGATTTCGATGTTTAGCGTCTTAGCGATCTCAACGCGCAAAATATCATCATAGGCAAGCCCTTTATTGAGATTAGCGTCGGGGGTGAGAG‑TGGTATTGGTGTGGTGAAAGCGACGGGAGTTaacggagcagcgcataccatttaaacggg                                                                                                                                                 >  2:33505/3‑228 (MQ=255)                                                                                                  tACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGGGGTGAAAGCGCCTGAAGTTAACGGAACGGCCCTGACCATTGGACCCGGc                                                                                                                                                >  2:91092/1‑251 (MQ=255)                                                                                                    ccTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGTAGTTAACGGAACGGCGCTGACCATTGAACCCggaac                                                                                                                                              >  2:2537/1‑248 (MQ=255)                                                                                                           cATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑GGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGtt                                                                                                                                        >  2:474511/1‑250 (MQ=255)                                                                                                             ttgatggttgatggCAATCAGCACGTGGAAGCACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGAGATTTAACTCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGCTTAGCGTCGGGGAATCGCG‑TGGGATTGGTGTGGTGAAAGAGCCTGAAGTTAACGGGACGGCGCTGGCCCATGAGCACGGCACCGGGtgtgg                                                                                                                                     >  2:302763/1‑247 (MQ=255)                                                                                                              tgatggttgatggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTag                                                                                                                                      >  1:190724/1‑249 (MQ=255)                                                                                                               gatggttgatggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAg                                                                                                                                                                                                                             >  1:127159/1‑162 (MQ=255)                                                                                                               gatggttgatggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAg                                                                                                                                                                                                                             <  2:127159/162‑1 (MQ=255)                                                                                                                        tggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCgggg                                                                                                                          >  2:115586/1‑250 (MQ=255)                                                                                                                         ggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCa                                                                                                                         <  1:270342/251‑1 (MQ=255)                                                                                                                                     gTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATGGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCTCCGGGTTAGAGCAAGGCGGGCAGGGCGTGCatat                                                                                                             <  2:435018/251‑1 (MQ=255)                                                                                                                                         aagaaCGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTTGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTGGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGCATTGGTGTGGTGCAAGCGCCTGAAGTTAACGGACCGGCGCTGACCATTGACCCCGGCACCGGGTTAGAGCAAGGCGGGCCGGGCgggccaatccct                                                                                                         >  1:354183/1‑240 (MQ=255)                                                                                                                                          agaaCGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCtt                                                                                                        <  2:236827/251‑1 (MQ=255)                                                                                                                                                   gATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAg                                                                                                                                                                                         <  2:228257/161‑1 (MQ=255)                                                                                                                                                   gATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAg                                                                                                                                                                                         >  1:228257/1‑161 (MQ=255)                                                                                                                                                    aTAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAgg                                                                                              >  1:77590/1‑251 (MQ=255)                                                                                                                                                    aTAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATACGCG‑TAGCCTTGGTGTGGTGACAGCCGCTGCAGTTAACGGCACGGCGCTGACCATTGAACCCGGCCACGGGGTACAGCAAGGTGGGGACGGCGTGGTTATCCCTTTAccccaa                                                                                                >  2:146644/1‑245 (MQ=255)                                                                                                                                                              ataCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCgg                                                                                    <  2:277960/251‑1 (MQ=255)                                                                                                                                                              ataCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCATCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCgg                                                                                    <  1:331689/251‑1 (MQ=255)                                                                                                                                                                    gTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCAAATCCCTTTACCCGAAGGGGACTGGAGGAAGCa                                                                               >  1:360391/1‑250 (MQ=255)                                                                                                                                                                      cAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCAGTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGaa                                                                            <  1:302763/251‑1 (MQ=255)                                                                                                                                                                              gCACAGTGAGTTAACGCTAAAAGCCGAGTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCGTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGc                                                                    <  2:485858/251‑1 (MQ=255)                                                                                                                                                                                          aaCGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGccc                                                        >  1:374090/1‑251 (MQ=255)                                                                                                                                                                                                     ccGAGTTCGATGTTGCGCGGCTTAGCGCGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGGTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGTTGAATATGGCCCTGAATTTacgc                                             <  2:259949/251‑4 (MQ=255)                                                                                                                                                                                                        aTTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGt                                          <  1:186780/251‑1 (MQ=255)                                                                                                                                                                                                          ttCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGGGCATATCCCTTTCCCCGAAGGGGACTGGCGGGAGCCGAACCTGAAGCTGGAAATGGCCCTGAGTTTAAACggaac                                        >  2:38607/1‑248 (MQ=255)                                                                                                                                                                                                                                agcTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCt                  <  1:243174/249‑1 (MQ=255)                                                                                                                                                                                                                                          gcCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTa        <  2:299555/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTATCATCTTAGGCAAGCCCTTTATTGGGATTAGCGTCGGGGATGCGCG‑TGGTAGTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATa     <  2:122133/251‑1 (MQ=255)                                                                                                                                                                                                                                                 atcatcTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCg  <  1:474511/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCG‑TGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCG  >  NC_000913/4638343‑4638832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑