Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,943	T→G	L416L (CTT→CTG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,943	0	T	G	95.5%	121.6 / ‑2.6	44	L416L (CTT→CTG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (22/20);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

GAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGA  >  NC_000913/4637710‑4638184                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTgca                                                                                                                                                                                                                                    >  1:412619/1‑247 (MQ=255)  aaGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGc                                                                                                                                                                                                                                 >  1:164434/1‑251 (MQ=255)          aCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                                                         <  2:82987/251‑1 (MQ=255)                              aaacaTCACTCTACGTGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCCGGAACACGTCGCCCTTAAGGTGCTGGCTACCGGCAGTGGTATTCCTGACTCCGCGCTGTCACGCATTTTTGACCGCTTTTACTCTTTGCCGCGTGCAACTGGGc                                                                                                                                                                                                     >  2:217494/5‑251 (MQ=255)                                          acATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGTAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagcagc                                                                                                                                                                                          <  1:187993/249‑1 (MQ=255)                                                   gCCCACTGATGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAAAGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGCGCGGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGt                                                                                                                                                                                <  2:188853/251‑1 (MQ=255)                                                    cccACTGAGGTTAATGGTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATACCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTGACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTTGGGtt                                                                                                                                                                               <  2:105340/251‑1 (MQ=255)                                                    cccACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTCTTTTTGAACGCTTTTACTCTTTGCCTCGTGCAACTGGGCAAAAAAGCAGCGGCCTGGGGtt                                                                                                                                                                               >  2:463796/1‑251 (MQ=255)                                                                       ctgctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGGATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAACAAGCATCGGCATGGGGTTGGCGTTCGTAAGTggggcc                                                                                                                                                            >  2:362077/1‑246 (MQ=255)                                                                        tgctgGACCGGCGTTAATGGAGCAGGCGCTGGGGAATTTAATGGATAACGACATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGAATACCGGTAGTGGTATTCCTGACTACGCGCTGTAACGTATTTTTGGACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGTGTTGGCGGTCGTCAGTGAGGTCg                                                                                                                                                           >  1:384338/1‑251 (MQ=255)                                                                            gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGCTTTTAACCCCGAGAGCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTTTTTTTGAAAGCTTTTACTCTTTGCCGCGTGCACATGGGCCACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGGGAGGTCGACCg                                                                                                                                                       >  2:391611/1‑251 (MQ=255)                                                                            gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGccc                                                                                                                                                        <  1:26447/250‑1 (MQ=255)                                                                                       aCTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAAc                                                                                                                                             <  2:164434/250‑1 (MQ=255)                                                                                       aCTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAAc                                                                                                                                             <  1:261996/250‑1 (MQ=255)                                                                                          ggAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGCGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGGGAGGTCGCCCGTTTGTTTACCGGCg                                                                                                                                         >  2:334665/1‑251 (MQ=255)                                                                                           gAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCg                                                                                                                                         >  1:293741/1‑250 (MQ=255)                                                                                                             ttACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                                                                      >  1:422271/1‑251 (MQ=255)                                                                                                               aCTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGTTAAGCGCTGAAGTGGATCAGGAACACGTCGTCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTAGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTg                                                                                                                     <  1:71124/250‑1 (MQ=255)                                                                                                                cTGGATAACGCCATCGATTTTACCCCCGAGAGCGTTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGcc                                                                                                                   >  2:42954/1‑250 (MQ=255)                                                                                                                  ggATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAgg                                                                                                                 >  1:471393/1‑251 (MQ=255)                                                                                                                      aaCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAg                                                                                                              <  1:472215/250‑1 (MQ=255)                                                                                                                          ccATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCAAGCTGCGCAACGTGCCGGAAGGTGGc                                                                                                         >  2:33388/1‑251 (MQ=255)                                                                                                                           cagcGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCg                                                                                                        <  1:249335/248‑1 (MQ=255)                                                                                                                                  tttACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGc                                                                                                 >  1:210438/1‑251 (MQ=255)                                                                                                                                        cccGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCt                                                                                           >  1:358506/1‑251 (MQ=255)                                                                                                                                            agagCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTTGATACCGGTAGTGGTATTCCGGACTACGCGCTGTCACGTATTTTTGAACGCTGTGACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGCTGGCGTTCGTCAGTGAGGTc                                                                                                                                                            <  2:344510/182‑1 (MQ=255)                                                                                                                                              atcggtcgcataatgCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGTTGCTGGATCCCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCCAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTATCGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACt                                                                                     <  1:250536/237‑1 (MQ=255)                                                                                                                                                    cgCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCAcc                                                                                <  2:293741/249‑1 (MQ=255)                                                                                                                                                               aaGCGACGAAGGGGATCAGGAACACGTCGCCCTTAAGGGGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGGCAAGTATTTGTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGGCTGGGGTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGGTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGAGTTCACCGTCACTTcaca                                                                     <  2:226249/250‑1 (MQ=255)                                                                                                                                                                   gcCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCt                                                                <  1:297947/251‑1 (MQ=255)                                                                                                                                                                     cGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTc                                                              >  1:115245/1‑251 (MQ=255)                                                                                                                                                                     cGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTc                                                              >  1:142945/1‑251 (MQ=255)                                                                                                                                                                       aaGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATCCCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCaa                                                            <  1:8215/251‑1 (MQ=255)                                                                                                                                                                                      acGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAATGCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAg                                             <  1:463796/251‑1 (MQ=255)                                                                                                                                                                                            cccTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCg                                       <  1:79667/249‑1 (MQ=255)                                                                                                                                                                                                   ggTGCGGGATACCGGTAGTGGTATTCCTGACGACGCGCTGTCACGTATTTTTGAACGCTTTTCCTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCctg                                <  2:213576/251‑1 (MQ=255)                                                                                                                                                                                                      gCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCctgctg                             >  1:357096/1‑251 (MQ=255)                                                                                                                                                                                                          gATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCAt                         >  1:338587/1‑251 (MQ=255)                                                                                                                                                                                                                gggagaggTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCAGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAACTTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCaa                    >  2:450438/7‑250 (MQ=255)                                                                                                                                                                                                                      ggTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCCCGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGGCTTCACCGTCACTTCACATAGCTTAAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAACGgaga              >  2:115766/1‑250 (MQ=255)                                                                                                                                                                                                                         aTTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTTACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGAc          <  1:66570/251‑1 (MQ=255)                                                                                                                                                                                                                               gACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGtt    <  2:338587/251‑1 (MQ=255)                                                                                                                                                                                                                                 cTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTg   >  1:109018/1‑250 (MQ=255)                                                                                                                                                                                                                                  tACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGa  >  1:319436/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  GAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGA  >  NC_000913/4637710‑4638184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑