Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,898	G→C	T401A (ACG→GCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,898	0	G	C	95.5%	130.4 / ‑2.7	44	T401A (ACG→GCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (22/20);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAG  >  NC_000913/4637653‑4638141                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTaa                                                                                                                                                                                                                                                <  1:227311/251‑1 (MQ=255)          ggcGTTCTGACTGTGGTGGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGa                                                                                                                                                                                                                                       <  2:304305/249‑1 (MQ=255)          gTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTggg                                                                                                                                                                                                                                       >  1:142718/1‑250 (MQ=255)             gTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGGTAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATATCGCTAAGCGCCGAAGGGGATCAGGAACAC‑GTCGCCCTTAAGGTGcgggttac                                                                                                                                                                                                                                    >  2:261996/1‑244 (MQ=255)               tCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCCCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGGGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGGGAGCGGTCGCCTAACGCTAAGCGCCCAAGTGGATCAGGAA‑ACAGTCCCCCTTAAGGTGCTGGATACCg                                                                                                                                                                                                                                  >  2:297947/1‑251 (MQ=255)                  gacgtttggtGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTa                                                                                                                                                                                                                               <  1:292956/242‑1 (MQ=255)                        tgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGGGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAAAGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACCATAAGCGCCGACGTGGATCAGGAACAC‑GTCTCCCTTAAGGTGCTGGGTACCggtggtggt                                                                                                                                                                                                                          >  2:472215/1‑250 (MQ=255)                               gtgGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTg                                                                                                                                                                                                                   >  1:82987/1‑250 (MQ=255)                                  gcggcATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACCC‑GTCGCCCTTAAGGGGCGGGATACCGGTCGTGGTATTCCTgcct                                                                                                                                                                                                                >  2:365739/1‑247 (MQ=255)                                          atttcgccgcGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCtgt                                                                                                                                                                                                       <  2:369328/247‑3 (MQ=255)                                                          gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTgca                                                                                                                                                                                         >  1:412619/1‑247 (MQ=255)                                                           aaGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGc                                                                                                                                                                                      >  1:164434/1‑251 (MQ=255)                                                                   aCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                              <  2:82987/251‑1 (MQ=255)                                                                                       aaacaTCACTCTACGTGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCCGGAACAC‑GTCGCCCTTAAGGTGCTGGCTACCGGCAGTGGTATTCCTGACTCCGCGCTGTCACGCATTTTTGACCGCTTTTACTCTTTGCCGCGTGCAACTGGGc                                                                                                                                                          >  2:217494/5‑251 (MQ=255)                                                                                                   acATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGTAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagcagc                                                                                                                                               <  1:187993/249‑1 (MQ=255)                                                                                                            gCCCACTGATGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAAAGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGCGCGGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGt                                                                                                                                     <  2:188853/251‑1 (MQ=255)                                                                                                             cccACTGAGGTTAATGGTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATACCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTGACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTTGGGtt                                                                                                                                    <  2:105340/251‑1 (MQ=255)                                                                                                             cccACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTCTTTTTGAACGCTTTTACTCTTTGCCTCGTGCAACTGGGCAAAAAAGCAGCGGCCTGGGGtt                                                                                                                                    >  2:463796/1‑251 (MQ=255)                                                                                                                                ctgctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGGATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAACAAGCATCGGCATGGGGTTGGCGTTCGTAAGTggggcc                                                                                                                 >  2:362077/1‑246 (MQ=255)                                                                                                                                 tgctgGACCGGCGTTAATGGAGCAGGCGCTGGGGAATTTAATGGATAACGACATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGAATACCGGTAGTGGTATTCCTGACTACGCGCTGTAACGTATTTTTGGACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGTGTTGGCGGTCGTCAGTGAGGTCg                                                                                                                >  1:384338/1‑251 (MQ=255)                                                                                                                                     gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGCTTTTAACCCCGAGAGCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTTTTTTTGAAAGCTTTTACTCTTTGCCGCGTGCACATGGGCCACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGGGAGGTCGACCg                                                                                                            >  2:391611/1‑251 (MQ=255)                                                                                                                                     gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGccc                                                                                                             <  1:26447/250‑1 (MQ=255)                                                                                                                                                aCTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAAc                                                                                                  <  1:261996/250‑1 (MQ=255)                                                                                                                                                aCTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAAc                                                                                                  <  2:164434/250‑1 (MQ=255)                                                                                                                                                   ggAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGCGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGGGAGGTCGCCCGTTTGTTTACCGGCg                                                                                              >  2:334665/1‑251 (MQ=255)                                                                                                                                                    gAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCg                                                                                              >  1:293741/1‑250 (MQ=255)                                                                                                                                                                      ttACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                           >  1:422271/1‑251 (MQ=255)                                                                                                                                                                        aCTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGTTAAGCGCTGAAGTGGATCAGGAACAC‑GTCGTCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTAGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTg                                                                          <  1:71124/250‑1 (MQ=255)                                                                                                                                                                         cTGGATAACGCCATCGATTTTACCCCCGAGAGCGTTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGcc                                                                        >  2:42954/1‑250 (MQ=255)                                                                                                                                                                           ggATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAgg                                                                      >  1:471393/1‑251 (MQ=255)                                                                                                                                                                               aaCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAg                                                                   <  1:472215/250‑1 (MQ=255)                                                                                                                                                                                   ccATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCAAGCTGCGCAACGTGCCGGAAGGTGGc                                                              >  2:33388/1‑251 (MQ=255)                                                                                                                                                                                    cagcGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCg                                                             <  1:249335/248‑1 (MQ=255)                                                                                                                                                                                           tttACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGc                                                      >  1:210438/1‑251 (MQ=255)                                                                                                                                                                                                 cccGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCt                                                >  1:358506/1‑251 (MQ=255)                                                                                                                                                                                                     agagCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTTGATACCGGTAGTGGTATTCCGGACTACGCGCTGTCACGTATTTTTGAACGCTGTGACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGCTGGCGTTCGTCAGTGAGGTc                                                                                                                 <  2:344510/182‑1 (MQ=255)                                                                                                                                                                                                       atcggtcgcataatgCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGTTGCTGGATCCCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCCAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTATCGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACt                                          <  1:250536/237‑1 (MQ=255)                                                                                                                                                                                                             cgCATAACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCAcc                                     <  2:293741/249‑1 (MQ=255)                                                                                                                                                                                                                        aaGCGACGAAGGGGATCAGGAACAC‑GTCGCCCTTAAGGGGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGGCAAGTATTTGTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGGCTGGGGTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGGTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGAGTTCACCGTCACTTcaca                          <  2:226249/250‑1 (MQ=255)                                                                                                                                                                                                                            gcCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCt                     <  1:297947/251‑1 (MQ=255)                                                                                                                                                                                                                              cGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTc                   >  1:142945/1‑251 (MQ=255)                                                                                                                                                                                                                              cGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTc                   >  1:115245/1‑251 (MQ=255)                                                                                                                                                                                                                                aaGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATCCCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCaa                 <  1:8215/251‑1 (MQ=255)                                                                                                                                                                                                                                               ac‑GTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAATGCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAg  <  1:463796/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAG  >  NC_000913/4637653‑4638141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑