Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,637,857 T→C C388R (TGC→CGC)  creC → sensory histidine kinase CreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,637,8570TC95.5% 121.3 / ‑2.0 45C388R (TGC→CGC) creCsensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (22/20);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

CCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCT  >  NC_000913/4637634‑4638095
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
ccAGGCAAGACTGGAGAATTGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGGGCAGGCGCTGGGAAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCAC‑GCTAAGCGCCGAAGTGGATc                                                                                                                                                                                                                      >  1:80466/1‑251 (MQ=255)
           tGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTc                                                                                                                                                                                                          >  1:20378/1‑251 (MQ=255)
            ggAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑Gt                                                                                                                                                                                                           <  1:10290/249‑1 (MQ=255)
            ggAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑Gt                                                                                                                                                                                                           <  1:346808/249‑1 (MQ=255)
            ggAGAATCGTCAGGAAGTCGTTCTGACTGTTATTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTcg                                                                                                                                                                                                         <  1:61592/251‑2 (MQ=255)
                   cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTcgccctta                                                                                                                                                                                                   <  1:162246/250‑8 (MQ=255)
                   cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTaa                                                                                                                                                                                                  <  1:227311/251‑1 (MQ=255)
                   cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGGCAGCGAAGCGCGCACCGTGCCGTTGGCAGAAAAAAACATCACTCTACCTGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCCGGCGCTGGGGAGTTTCAC‑GGATAACGCCATCGATTTTACCCCCGAGAGCGGGAGCAT‑ACAGCTAAGCGCCGAAggggcaaaaggacacgtcgccctta                                                                                                                                                                                                    >  2:399190/1‑226 (MQ=255)
                            ggcGTTCTGACTGTGGTGGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGa                                                                                                                                                                                         <  2:304305/249‑1 (MQ=255)
                            gTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTggg                                                                                                                                                                                         >  1:142718/1‑250 (MQ=255)
                               gTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGGTAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATATC‑GCTAAGCGCCGAAGGGGATCAGGAACAC‑GTCGCCCTTAAGGTGcgggttac                                                                                                                                                                                      >  2:261996/1‑244 (MQ=255)
                                 tCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCCCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGGGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGGGAGCGGTCGCCTAAC‑GCTAAGCGCCCAAGTGGATCAGGAA‑ACAGTCCCCCTTAAGGTGCTGGATACCg                                                                                                                                                                                    >  2:297947/1‑251 (MQ=255)
                                    gacgtttggtGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTa                                                                                                                                                                                 <  1:292956/242‑1 (MQ=255)
                                          tgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGGGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAAAGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑CATAAGCGCCGACGTGGATCAGGAACAC‑GTCTCCCTTAAGGTGCTGGGTACCggtggtggt                                                                                                                                                                            >  2:472215/1‑250 (MQ=255)
                                                 gtgGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTg                                                                                                                                                                     >  1:82987/1‑250 (MQ=255)
                                                    gcggcATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACCC‑GTCGCCCTTAAGGGGCGGGATACCGGTCGTGGTATTCCTgcct                                                                                                                                                                  >  2:365739/1‑247 (MQ=255)
                                                            atttcgccgcGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCtgt                                                                                                                                                         <  2:369328/247‑3 (MQ=255)
                                                                            gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTgca                                                                                                                                           >  1:412619/1‑247 (MQ=255)
                                                                             aaGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGc                                                                                                                                        >  1:164434/1‑251 (MQ=255)
                                                                                cgcgcACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGaa                                                                                                                                                                                                                             <  2:9114/164‑1 (MQ=255)
                                                                                cgcgcACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGaa                                                                                                                                                                                                                             >  1:9114/1‑164 (MQ=255)
                                                                                     aCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                <  2:82987/251‑1 (MQ=255)
                                                                                                         aaacaTCACTCTACGTGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCCGGAACAC‑GTCGCCCTTAAGGTGCTGGCTACCGGCAGTGGTATTCCTGACTCCGCGCTGTCACGCATTTTTGACCGCTTTTACTCTTTGCCGCGTGCAACTGGGc                                                                                                            >  2:217494/5‑251 (MQ=255)
                                                                                                                     acATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGTAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagcagc                                                                                                 <  1:187993/249‑1 (MQ=255)
                                                                                                                              gCCCACTGATGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAAAGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGCGCGGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGt                                                                                       <  2:188853/251‑1 (MQ=255)
                                                                                                                               cccACTGAGGTTAATGGTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATACCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTGACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTTGGGtt                                                                                      <  2:105340/251‑1 (MQ=255)
                                                                                                                               cccACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTT‑ACTGGCTAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTCTTTTTGAACGCTTTTACTCTTTGCCTCGTGCAACTGGGCAAAAAAGCAGCGGCCTGGGGtt                                                                                      >  2:463796/1‑251 (MQ=255)
                                                                                                                                                  ctgctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGGATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAACAAGCATCGGCATGGGGTTGGCGTTCGTAAGTggggcc                                                                   >  2:362077/1‑246 (MQ=255)
                                                                                                                                                   tgctgGACCGGCGTTAATGGAGCAGGCGCTGGGGAATTT‑AATGGATAACGACATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGAATACCGGTAGTGGTATTCCTGACTACGCGCTGTAACGTATTTTTGGACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGTGTTGGCGGTCGTCAGTGAGGTCg                                                                  >  1:384338/1‑251 (MQ=255)
                                                                                                                                                       gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGCTTTTAACCCCGAGAGCGGGCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTTTTTTTGAAAGCTTTTACTCTTTGCCGCGTGCACATGGGCCACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGGGAGGTCGACCg                                                              >  2:391611/1‑251 (MQ=255)
                                                                                                                                                       gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGccc                                                               <  1:26447/250‑1 (MQ=255)
                                                                                                                                                                  aCTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAAc                                                    <  2:164434/250‑1 (MQ=255)
                                                                                                                                                                  aCTGGAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAAc                                                    <  1:261996/250‑1 (MQ=255)
                                                                                                                                                                     ggAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGCGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGGGAGGTCGCCCGTTTGTTTACCGGCg                                                >  2:334665/1‑251 (MQ=255)
                                                                                                                                                                      gAGCAGGCGCTGGGGAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCg                                                >  1:293741/1‑250 (MQ=255)
                                                                                                                                                                                        tt‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                             >  1:422271/1‑251 (MQ=255)
                                                                                                                                                                                           aCTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GTTAAGCGCTGAAGTGGATCAGGAACAC‑GTCGTCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTAGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTg                            <  1:71124/250‑1 (MQ=255)
                                                                                                                                                                                            cTGGATAACGCCATCGATTTTACCCCCGAGAGCGTTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGAAACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGcc                          >  2:42954/1‑250 (MQ=255)
                                                                                                                                                                                              ggATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAgg                        >  1:471393/1‑251 (MQ=255)
                                                                                                                                                                                                  aaCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAg                     <  1:472215/250‑1 (MQ=255)
                                                                                                                                                                                                      ccATCGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCAAGCTGCGCAACGTGCCGGAAGGTGGc                >  2:33388/1‑251 (MQ=255)
                                                                                                                                                                                                       cagcGATTTTACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCg               <  1:249335/248‑1 (MQ=255)
                                                                                                                                                                                                              tttACCCCCGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGc        >  1:210438/1‑251 (MQ=255)
                                                                                                                                                                                                                    cccGAGAGCGGTCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCt  >  1:358506/1‑251 (MQ=255)
                                                                                                                                                                                                                        agagCGGGCGCATAAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCGCCCTTAAGGTGCTTGATACCGGTAGTGGTATTCCGGACTACGCGCTGTCACGTATTTTTGAACGCTGTGACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGCTGGCGTTCGTCAGTGAGGTc                                                                   <  2:344510/182‑1 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
CCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTT‑ACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCAC‑GCTAAGCGCCGAAGTGGATCAGGAACAC‑GTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCT  >  NC_000913/4637634‑4638095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑