Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,478	C→T	Y261Y (TAC→TAT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,478	0	C	T	93.3%	124.4 / ‑1.5	45	Y261Y (TAC→TAT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (20/22);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCG  >  NC_000913/4637236‑4637722                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      gagcgGCGAATTTTATTGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAAc                                                                                                                                                                                                                                               <  1:310056/251‑1 (MQ=255)        gAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATg                                                                                                                                                                                                                                          >  1:63616/1‑249 (MQ=255)                       gcgcCATTTTTCTTGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATTAGCTAAAAAGCCCACTg                                                                                                                                                                                                                         <  2:215002/251‑1 (MQ=255)                          ccATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTggcg                                                                                                                                                                                                                      <  2:239357/251‑1 (MQ=255)                            aTTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGACGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTggcgg‑c                                                                                                                                                                                                                   >  2:292956/1‑251 (MQ=255)                                tgctgGGGATTGCACTGGTTATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACGATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACGGGCGG‑CGAt                                                                                                                                                                                                                <  2:147735/247‑1 (MQ=255)                                tgctgGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGAt                                                                                                                                                                                                                <  1:273551/247‑1 (MQ=255)                                    ggggATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGt                                                                                                                                                                                                            <  1:60250/250‑1 (MQ=255)                                     gggATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGTATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTgg                                                                                                                                                                                                          <  2:485908/251‑1 (MQ=255)                                           gCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑GGATTCGTggcacagg                                                                                                                                                                                                    >  2:227311/1‑245 (MQ=255)                                                ggTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGAtt                                                                                                                                                                                                               <  2:342955/235‑1 (MQ=255)                                                ggTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGAtt                                                                                                                                                                                                               >  1:342955/1‑235 (MQ=255)                                                              gCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTAGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAACTAAAAAGACCACTGGCGGACG‑TTCGTGGCTCGGCGGCAATATTACGCGAAggg                                                                                                                                                                                 >  1:18874/1‑250 (MQ=255)                                                                 tGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGCGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGAGAATGTccc                                                                                                                                                                              >  2:71655/1‑250 (MQ=255)                                                                 tGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTccg                                                                                                                                                                              >  2:462168/1‑251 (MQ=255)                                                                                 ccGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGtggtgg                                                                                                                                                              <  1:224559/251‑1 (MQ=255)                                                                                           gCCCGGCTCCCCCGCTATGCCGATTCCGTCACTGAAAATAAGCCCGTTCCTCTTCCTGAACTCGTCAGTAGCGAGTTGCGTAAGCGTGCGCAGGCGCTGGAAAGTATGCGCGTTAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCTGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGttttgc                                                                                                                                                    <  2:84127/251‑3 (MQ=255)                                                                                            cccGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTAcc                                                                                                                                                   <  1:254377/251‑2 (MQ=255)                                                                                            cccGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTAcc                                                                                                                                                   >  1:133891/1‑250 (MQ=255)                                                                                                 cTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCCGGCGCTGGAAGGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGAAGCGGCGGAAATTTTACGCGCAGGTCCGCCGCCGGACGGGGTGGCTCGtttttattgca                                                                                                                                               >  2:232207/1‑243 (MQ=255)                                                                                                   cccTCGCTATGCCGATTCCGTCACTTACAATAAGCCCGTTCCTCTTCCGGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCTGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGacaaca                                                                                                                                            <  2:273576/249‑1 (MQ=255)                                                                                                    aCTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGCAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGACCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACAt                                                                                                                                           >  1:32124/1‑251 (MQ=255)                                                                                                          tATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAgt                                                                                                                                                                   <  2:87715/221‑1 (MQ=255)                                                                                                          tATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAgt                                                                                                                                                                   >  1:87715/1‑221 (MQ=255)                                                                                                                gATTCCGGCCCTGACAATAAGCCTGTTCCTCGTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCaaaa                                                                                                                               <  2:148411/251‑1 (MQ=255)                                                                                                                   tCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCCGGCGCTGGAAAGTATGCGCGTGACGCTGGAAGGGCAACACTATATTGCGCAGTATGTTTATGCGTTAACCCATGAGCTAACAAGCCCACTGGCCG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGACGTGGTGACTCGTTTTACCGACACCATTCTGACGCAACATgc                                                                                                                            >  1:241752/1‑251 (MQ=255)                                                                                                                      gTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCCACGGTCCGCCACCTGGAGGGGGGGCGCGTTTTACCGACACAATTCTGACGCAAAATGcgcg                                                                                                                         >  2:10290/1‑251 (MQ=255)                                                                                                                      gTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGcgcg                                                                                                                         <  1:232207/251‑1 (MQ=255)                                                                                                                                aTAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGc                                                                                                                >  1:290244/1‑250 (MQ=255)                                                                                                                                       cGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGCCGCAAAATGCGCGTATGCAGGCATTGGt                                                                                                          >  2:61592/1‑249 (MQ=255)                                                                                                                                                      actcGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGTCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGcc                                                                                         >  2:110494/2‑251 (MQ=255)                                                                                                                                                                                      aGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGtt                                                         <  1:289172/251‑1 (MQ=255)                                                                                                                                                                                        gcgcTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGTCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCt                                                       <  1:110494/251‑1 (MQ=255)                                                                                                                                                                                            tGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGCAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGAAGCAAAATGCGCGTATGCAGGCATTGGACGAAACGTTACTACGCCAGGCAAGACTGGAGAATAGTCAGGAAGTCGTTCTggat                                                   >  1:277647/1‑248 (MQ=255)                                                                                                                                                                                                 aGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCTCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGGTCGTTTTACCGACAACATTCTGACGCAAAATGCCCGCATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGCCtgttgt                                              >  2:183914/1‑251 (MQ=255)                                                                                                                                                                                                              aGCTGGAAGGGAAAAACTATATTGAGCAGTATGGTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGTTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGCTCTGACTGTTGGTGATGTGGCGGCa                                 <  1:341816/251‑1 (MQ=255)                                                                                                                                                                                                               gcctgAAGGGAAAAACTATATTTAGCAGTTTGTTTATGCGGTAACCCAAGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTAACGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCat                                <  2:316862/247‑1 (MQ=255)                                                                                                                                                                                                                  ggAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattat                             >  1:173004/1‑251 (MQ=255)                                                                                                                                                                                                                  ggAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCatt                               <  1:181083/249‑1 (MQ=255)                                                                                                                                                                                                                      gggAAAAACTATATTGAGCAGGATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGTGCCGCCACCGGAAGTTGTGGCGCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGGTCTGACTGTTGTTGATGTGGCGGCattatttcg                         <  2:450304/251‑4 (MQ=255)                                                                                                                                                                                                                         aaaaaCTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTtcgccg                      <  1:75796/251‑1 (MQ=255)                                                                                                                                                                                                                            aaCTATATTGAGCAGTATGTTTATGCGTTAACCCCTGAGCTAAAAAGCCCACTGGAGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGAGGTGGCTCGTTTTAACGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAATACTGGGAAATCGTATGGGAGTCGTTCTGACTGTTGTTGGTGTGGCGGCaatttttttccgcac                   >  2:79667/1‑237 (MQ=255)                                                                                                                                                                                                                             aCTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTc                  >  1:369328/1‑251 (MQ=255)                                                                                                                                                                                                                                atatTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGc               <  2:404134/251‑1 (MQ=255)                                                                                                                                                                                                                                              gTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCg  <  1:71655/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGG‑CGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCG  >  NC_000913/4637236‑4637722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑