Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,767	C→T	A24A (GCC→GCT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,767	0	C	T	100.0%	146.7 / NA	51	A24A (GCC→GCT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

CCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTG  >  NC_000913/4636530‑4637010                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     cccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCaa                                                                                                                                                                                                                                           >  1:330803/1‑249 (MQ=255)    gCCAGCAACTAATGGATTGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATCCTTATCGCGGCAGGGGATATAGCCTGAGTGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAaagaag                                                                                                                                                                                                                                      <  2:290268/251‑1 (MQ=255)     ccAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGt                                                                                                                                                                                                                                     <  1:228925/251‑1 (MQ=255)              aaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGACTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGGGGCGGTGGCGGCCTGGGTTTTACTGGCTAttttttttgacgaggttgaaccggg                                                                                                                                                                                                                            >  2:402081/2‑231 (MQ=255)                       cgtgtGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTTCAAGAAGTTAAACCGGGCGTGCgg                                                                                                                                                                                                                     >  2:219555/5‑248 (MQ=255)                             ggAAGATGCGCAGGACACCTACGAGCGCACCGGTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATGAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAc                                                                                                                                                                                                             <  2:357079/251‑1 (MQ=255)                                    gcgcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGTAgggg                                                                                                                                                                                                      <  2:452621/251‑1 (MQ=255)                                      gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAc                                                                                                                                                                                                    <  1:480911/251‑1 (MQ=255)                                      gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAc                                                                                                                                                                                                    <  1:308400/251‑1 (MQ=255)                                                  cGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGatac                                                                                                                                                                                        <  1:435350/251‑4 (MQ=255)                                                   gATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATAcc                                                                                                                                                                                       >  1:308405/1‑251 (MQ=255)                                                      cGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCAGCTCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTCCTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGGGAAGAGCAACGGAGGGGGAGTTAATCGATACCGc                                                                                                                                                                                     >  2:66488/1‑250 (MQ=255)                                                        cACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGGACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAAGTTAATCGATACCGCAAc                                                                                                                                                                                  >  1:130788/1‑251 (MQ=255)                                                                            aaaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑Agactgccc                                                                                                                                                             >  1:84127/1‑244 (MQ=255)                                                                            aaaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGgcgc                                                                                                                                                             >  1:475867/1‑251 (MQ=255)                                                                             aaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGAGCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGcgcg                                                                                                                                                            <  1:267636/251‑1 (MQ=255)                                                                               cgttacGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGGTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGcgcggc                                                                                                                                                          >  2:471028/6‑249 (MQ=255)                                                                                    cGTGCCTAGCTGCGCGCCATCAACCCTGATCTTGCACCGATGAATACTCATCGCGGAAGGGGAGATAGCCTGAGGGGCCAGTAAAGCGTATCGGCATGCGGTTGCTGCTAGGCTATTTTCTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATGTGTGTCAAAGAAGTTAAGCCGGGCTTGAGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGAt                                                                                                                                                     <  2:372759/251‑1 (MQ=255)                                                                                      tGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGAttt                                                                                                                                                   >  1:208109/1‑251 (MQ=255)                                                                                        ccAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGCGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGGAGGGCAACGGAGGGGGCGTTAATCGATACCGCAAAGTTGCTGGCGG‑AGCAGGGGCGTCCCGCTTTgg                                                                                                                                                 >  2:243336/1‑250 (MQ=255)                                                                                                   gcCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGAc                                                                                                                                      >  1:347449/1‑251 (MQ=255)                                                                                                      aTCAACCCCGATCTTTCACCGATTAATCCTCATCGCGGCATGGGATATAGACTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTGGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAAGGCAACGGGGGGGAAGTTAAGGGCTACGTCAACGTGGCTGGGGG‑AGTTGGTGCGTCCCGATTTGccgtcttggggccc                                                                                                                                    >  1:186254/1‑237 (MQ=255)                                                                                                      aTCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTTTTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCCACGGAGGGGATGTTAATCGATACCGCAACGTTGCTGGCGGGAG‑TGGCGCGTCCCGAttttgtccctgggggacc                                                                                                                                    >  2:391755/1‑235 (MQ=255)                                                                                                               gATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGAATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTACGTACTGGCTATTTTTGTCAAAGAAGATAATCCGGGAGTGCGAAGAGCAAAGGAGGGGACGTTAATCGCTACCGCAAAGTTGCTGGCGG‑GACTGGCGCGTCCCGGTTTGCTCCCTGGGGAGCCAACgaccggg                                                                                                                          >  2:430263/1‑245 (MQ=255)                                                                                                                             tAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTAATCGCTACCGCCACGTTGCTGGCGG‑AGCTGGGGCGTGCCAGTTTGCTCTCTGGGGACCCACCCCCTGGGCAAcaggggcgggg                                                                                                            >  2:373982/1‑241 (MQ=255)                                                                                                                                   tCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAc                                                                                                                                 >  1:411675/1‑224 (MQ=255)                                                                                                                                   tCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAc                                                                                                                                 <  2:411675/224‑1 (MQ=255)                                                                                                                                       ggcgGCATGGGATATAGGCTGAGGGGGCTGTAATGCGTATCGGCATGCGGGTGCTGCTGGGCTATTTTTGTCTGGTGGCGGTGGCGGCCTGGTTCGTAATGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGATGGGACGTTAAGCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAg                                                                                                  <  2:240606/250‑1 (MQ=255)                                                                                                                                             aTGGGATATAGCCTGAGGGGCCTGTAATGCGTAGCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTacaa                                                                                            <  1:335164/251‑1 (MQ=255)                                                                                                                                               gggttataGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTacaac                                                                                           <  2:347449/246‑1 (MQ=255)                                                                                                                                                 gATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGTGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATc                                                                                        <  1:331702/251‑1 (MQ=255)                                                                                                                                                  atatAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑GGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCCAACGCCTGGGCAACTGGCGCAGGAGTTTAATCAACTAAAACATCg                                                                                       >  1:164500/1‑251 (MQ=255)                                                                                                                                                   tataGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGGAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCCAAGTTGCTGGCGG‑AGCTGGCGCGTCCCGCTTTGCTCTCTGGGGACCCAACCCATGGGGCACAGGCGCAGGCGTTTAATACGCTACAACATCGc                                                                                      >  2:8785/1‑251 (MQ=255)                                                                                                                                                           gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTCCTGGCTATTTTTGTCCAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGATAATCGATACCGCAACGTTGCTGGCGG‑CGCCGGCGCGGCCCGAGTTGCTCTCTGGGGACCCAAACCATGGGCAACTGGCCCAGGCGGTTAATCCGCTTCAACAACGCCCGtttgc                                                                              >  2:209857/1‑249 (MQ=255)                                                                                                                                                           gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTc                                                                               <  1:180867/250‑1 (MQ=255)                                                                                                                                                                      aaTGCGTATCGGCATGCGGGTGCTGCTGGGCTATTTTTTACTGGTGGCGGTTGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTGGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCg                                                                   <  1:66488/251‑1 (MQ=255)                                                                                                                                                                                      cGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGTCGTTAATCGCTACCGCACCGTTGCTGGCGG‑GGCTGGCGCGTTCCGATTTTCTCTCTTGGGGCCCCACCCCTGGGCAAATGGCGCAGGCGTTTTATCCGCTACCACCTCGCCCGTTTCCCGCCAATTTCTGTGGcctttaacatat                                                   >  2:51265/1‑240 (MQ=255)                                                                                                                                                                                              gCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAAt                                            <  1:75592/250‑1 (MQ=255)                                                                                                                                                                                                        tttttACGGGGGGCGGGGGCGGCCTGGTTCGTACTGGCTAGTTGTGTCAAAGAAGTTAACCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATg                                  <  2:263835/250‑1 (MQ=255)                                                                                                                                                                                                                 gtggcggtggcgGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGAc                        <  1:219555/251‑1 (MQ=255)                                                                                                                                                                                                                    gcggaggcGGCCTGGTTCGTACTGGCTATTTTTGTAAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAACGTGCGCAATGAATATCATGTCTATATGACCg                      >  1:407765/1‑250 (MQ=255)                                                                                                                                                                                                                      ggtggcGGCCTTGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGGGCAACGGAGGGGACGTTAATCGATACCGCAACGTGGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGTATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGAtt                   <  2:262699/251‑2 (MQ=255)                                                                                                                                                                                                                         ggcggCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGAttcg                 >  1:74134/5‑247 (MQ=255)                                                                                                                                                                                                                         ggcggCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGAttcg                 <  1:352122/246‑4 (MQ=255)                                                                                                                                                                                                                         ggcggCCTGGTTCGTACTGGCTAGTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTGGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGAttcg                 <  2:74134/246‑4 (MQ=255)                                                                                                                                                                                                                                  gTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAgggtaa         >  1:148411/1‑246 (MQ=255)                                                                                                                                                                                                                                  gTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAg       <  1:471028/251‑1 (MQ=255)                                                                                                                                                                                                                                   ttCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCa               <  2:257080/242‑1 (MQ=255)                                                                                                                                                                                                                                   ttCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCa               >  1:257080/1‑242 (MQ=255)                                                                                                                                                                                                                                       tACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCCATATCGGTGGCCTTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTg  >  2:212171/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTg  <  1:366035/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     CCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTG  >  NC_000913/4636530‑4637010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑