Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,749	A→G	A18A (GCA→GCG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,749	0	A	G	100.0%	134.1 / NA	45	A18A (GCA→GCG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/22);  total (23/22)

CACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATG  >  NC_000913/4636512‑4636993                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      ccccgggcccgttCTGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGAGCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTAc                                                                                                                                                                                                                                          <  2:385251/239‑1 (MQ=255)        ccgcgcttggccccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGctat                                                                                                                                                                                                                                   <  1:12347/240‑4 (MQ=255)                   cccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCaa                                                                                                                                                                                                                          >  1:330803/1‑249 (MQ=255)                      gCCAGCAACTAATGGATTGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATCCTTATCGCGGCAGGGGATATAGCCTGAGTGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAaagaag                                                                                                                                                                                                                     <  2:290268/251‑1 (MQ=255)                       ccAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGt                                                                                                                                                                                                                    <  1:228925/251‑1 (MQ=255)                                aaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGACTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGGGGCGGTGGCGGCCTGGGTTTTACTGGCTAttttttttgacgaggttgaaccggg                                                                                                                                                                                                           >  2:402081/2‑231 (MQ=255)                                         cgtgtGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTTCAAGAAGTTAAACCGGGCGTGCgg                                                                                                                                                                                                    >  2:219555/5‑248 (MQ=255)                                               ggAAGATGCGCAGGACACCTACGAGCGCACCGGTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATGAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAc                                                                                                                                                                                            <  2:357079/251‑1 (MQ=255)                                                      gcgcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGTAgggg                                                                                                                                                                                     <  2:452621/251‑1 (MQ=255)                                                        gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAc                                                                                                                                                                                   <  1:480911/251‑1 (MQ=255)                                                        gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAc                                                                                                                                                                                   <  1:308400/251‑1 (MQ=255)                                                                    cGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGatac                                                                                                                                                                       <  1:435350/251‑4 (MQ=255)                                                                     gATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATAcc                                                                                                                                                                      >  1:308405/1‑251 (MQ=255)                                                                        cGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGct                                                                                                                                                                                                                                     >  2:163711/1‑184 (MQ=255)                                                                        cGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGct                                                                                                                                                                                                                                     <  1:163711/185‑2 (MQ=255)                                                                        cGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCAGCTCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTCCTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGGGAAGAGCAACGGAGGGGGAGTTAATCGATACCGc                                                                                                                                                                    >  2:66488/1‑250 (MQ=255)                                                                          cACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGGACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAAGTTAATCGATACCGCAAc                                                                                                                                                                 >  1:130788/1‑251 (MQ=255)                                                                                              aaaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑Agactgccc                                                                                                                                            >  1:84127/1‑244 (MQ=255)                                                                                              aaaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGgcgc                                                                                                                                            >  1:475867/1‑251 (MQ=255)                                                                                               aaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGAGCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGcgcg                                                                                                                                           <  1:267636/251‑1 (MQ=255)                                                                                                 cgttacGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGGTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGcgcggc                                                                                                                                         >  2:471028/6‑249 (MQ=255)                                                                                                      cGTGCCTAGCTGCGCGCCATCAACCCTGATCTTGCACCGATGAATACTCATCGCGGAAGGGGAGATAGCCTGAGGGGCCAGTAAAGCGTATCGGCATGCGGTTGCTGCTAGGCTATTTTCTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATGTGTGTCAAAGAAGTTAAGCCGGGCTTGAGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGAt                                                                                                                                    <  2:372759/251‑1 (MQ=255)                                                                                                        tGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGAttt                                                                                                                                  >  1:208109/1‑251 (MQ=255)                                                                                                          ccAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGCGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGGAGGGCAACGGAGGGGGCGTTAATCGATACCGCAAAGTTGCTGGCGG‑AGCAGGGGCGTCCCGCTTTgg                                                                                                                                >  2:243336/1‑250 (MQ=255)                                                                                                                     gcCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGAc                                                                                                                     >  1:347449/1‑251 (MQ=255)                                                                                                                        aTCAACCCCGATCTTTCACCGATTAATCCTCATCGCGGCATGGGATATAGACTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTGGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAAGGCAACGGGGGGGAAGTTAAGGGCTACGTCAACGTGGCTGGGGG‑AGTTGGTGCGTCCCGATTTGccgtcttggggccc                                                                                                                   >  1:186254/1‑237 (MQ=255)                                                                                                                        aTCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTTTTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCCACGGAGGGGATGTTAATCGATACCGCAACGTTGCTGGCGGGAG‑TGGCGCGTCCCGAttttgtccctgggggacc                                                                                                                   >  2:391755/1‑235 (MQ=255)                                                                                                                                 gATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGAATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTACGTACTGGCTATTTTTGTCAAAGAAGATAATCCGGGAGTGCGAAGAGCAAAGGAGGGGACGTTAATCGCTACCGCAAAGTTGCTGGCGG‑GACTGGCGCGTCCCGGTTTGCTCCCTGGGGAGCCAACgaccggg                                                                                                         >  2:430263/1‑245 (MQ=255)                                                                                                                                               tAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTAATCGCTACCGCCACGTTGCTGGCGG‑AGCTGGGGCGTGCCAGTTTGCTCTCTGGGGACCCACCCCCTGGGCAAcaggggcgggg                                                                                           >  2:373982/1‑241 (MQ=255)                                                                                                                                                     tCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAc                                                                                                                <  2:411675/224‑1 (MQ=255)                                                                                                                                                     tCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAc                                                                                                                >  1:411675/1‑224 (MQ=255)                                                                                                                                                         ggcgGCATGGGATATAGGCTGAGGGGGCTGTAATGCGTATCGGCATGCGGGTGCTGCTGGGCTATTTTTGTCTGGTGGCGGTGGCGGCCTGGTTCGTAATGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGATGGGACGTTAAGCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAg                                                                                 <  2:240606/250‑1 (MQ=255)                                                                                                                                                               aTGGGATATAGCCTGAGGGGCCTGTAATGCGTAGCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTacaa                                                                           <  1:335164/251‑1 (MQ=255)                                                                                                                                                                 gggttataGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTacaac                                                                          <  2:347449/246‑1 (MQ=255)                                                                                                                                                                   gATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGTGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATc                                                                       <  1:331702/251‑1 (MQ=255)                                                                                                                                                                    atatAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑GGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCCAACGCCTGGGCAACTGGCGCAGGAGTTTAATCAACTAAAACATCg                                                                      >  1:164500/1‑251 (MQ=255)                                                                                                                                                                     tataGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGGAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCCAAGTTGCTGGCGG‑AGCTGGCGCGTCCCGCTTTGCTCTCTGGGGACCCAACCCATGGGGCACAGGCGCAGGCGTTTAATACGCTACAACATCGc                                                                     >  2:8785/1‑251 (MQ=255)                                                                                                                                                                             gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTCCTGGCTATTTTTGTCCAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGATAATCGATACCGCAACGTTGCTGGCGG‑CGCCGGCGCGGCCCGAGTTGCTCTCTGGGGACCCAAACCATGGGCAACTGGCCCAGGCGGTTAATCCGCTTCAACAACGCCCGtttgc                                                             >  2:209857/1‑249 (MQ=255)                                                                                                                                                                             gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTc                                                              <  1:180867/250‑1 (MQ=255)                                                                                                                                                                                        aaTGCGTATCGGCATGCGGGTGCTGCTGGGCTATTTTTTACTGGTGGCGGTTGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTGGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCg                                                  <  1:66488/251‑1 (MQ=255)                                                                                                                                                                                                        cGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGTCGTTAATCGCTACCGCACCGTTGCTGGCGG‑GGCTGGCGCGTTCCGATTTTCTCTCTTGGGGCCCCACCCCTGGGCAAATGGCGCAGGCGTTTTATCCGCTACCACCTCGCCCGTTTCCCGCCAATTTCTGTGGcctttaacatat                                  >  2:51265/1‑240 (MQ=255)                                                                                                                                                                                                                gCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAAt                           <  1:75592/250‑1 (MQ=255)                                                                                                                                                                                                                          tttttACGGGGGGCGGGGGCGGCCTGGTTCGTACTGGCTAGTTGTGTCAAAGAAGTTAACCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATg                 <  2:263835/250‑1 (MQ=255)                                                                                                                                                                                                                                   gtggcggtggcgGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGAc       <  1:219555/251‑1 (MQ=255)                                                                                                                                                                                                                                      gcggaggcGGCCTGGTTCGTACTGGCTATTTTTGTAAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAACGTGCGCAATGAATATCATGTCTATATGACCg     >  1:407765/1‑250 (MQ=255)                                                                                                                                                                                                                                        ggtggcGGCCTTGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGGGCAACGGAGGGGACGTTAATCGATACCGCAACGTGGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGTATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGAtt  <  2:262699/251‑2 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATG  >  NC_000913/4636512‑4636993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑