Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,717	T→C	L8L (TTG→CTG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,717	0	T	C	100.0%	153.8 / NA	48	L8L (TTG→CTG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/20);  total (28/20)

TGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGT  >  NC_000913/4636470‑4636961                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATAGCGGCATGGGATATAGCCTGCGGGCACGGTAATGCGTATCGGACTGCGGTtgctgc                                                                                                                                                                                                                                                    >  2:267636/1‑251 (MQ=255)          aTGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGCTATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtt                                                                                                                                                                                                                                           >  2:480911/1‑251 (MQ=255)            gAGTTTTTATTGCTGAAGACGGTACCCAAGTCGCCAGTCGGGGTCGTGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATTCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtttt                                                                                                                                                                                                                                         <  1:30569/251‑1 (MQ=255)              gTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGCAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGAATCGGCATGCGGTTGCTGCTGGGCTAtttttt                                                                                                                                                                                                                                       >  1:452621/1‑251 (MQ=255)              gTTTTTATTGCTGAAGACGTTACTAAAGTAGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtttttt                                                                                                                                                                                                                                       >  1:394760/1‑251 (MQ=255)               tttttATTGCTGAAGGCGTTCCTCAAGGAGCCCTGCCGCGACTGGTCCAGCCAGCAACTAATGGCTAGCGAGTGGGAAGATGCGCAGGACACCTACGATCGCAACGTTGATAACCACATTAAAACGGTACGTGCCAAGCTGAGCGGCATCAACCCCCATCTTTCACCGATTAATACTCATCGCGGCATGGGATAAAGCCTGGAGGGACTGAAATTCGGACCGGAATGCGGTGGCTGCATGGCTAtttttgg                                                                                                                                                                                                                                      >  2:325083/1‑249 (MQ=255)                               cGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcggtggcgg                                                                                                                                                                                                                      >  1:385251/1‑249 (MQ=255)                                      cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATACGGTTGCTGCTGGGCTATTTTTTATTggtggaggtggcggaccgggt                                                                                                                                                                                                               >  2:381647/1‑242 (MQ=255)                                           ccccgggcccgttCTGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGAGCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTAc                                                                                                                                                                                                          <  2:385251/239‑1 (MQ=255)                                                  ccgcgcttggccccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGctat                                                                                                                                                                                                   <  1:12347/240‑4 (MQ=255)                                                             cccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCaa                                                                                                                                                                                          >  1:330803/1‑249 (MQ=255)                                                                gCCAGCAACTAATGGATTGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATCCTTATCGCGGCAGGGGATATAGCCTGAGTGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAaagaag                                                                                                                                                                                     <  2:290268/251‑1 (MQ=255)                                                                 ccAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGt                                                                                                                                                                                    <  1:228925/251‑1 (MQ=255)                                                                          aaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGACTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGGGGCGGTGGCGGCCTGGGTTTTACTGGCTAttttttttgacgaggttgaaccggg                                                                                                                                                                           >  2:402081/2‑231 (MQ=255)                                                                                   cgtgtGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTTCAAGAAGTTAAACCGGGCGTGCgg                                                                                                                                                                    >  2:219555/5‑248 (MQ=255)                                                                                         ggAAGATGCGCAGGACACCTACGAGCGCACCGGTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATGAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAc                                                                                                                                                            <  2:357079/251‑1 (MQ=255)                                                                                                gcgcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGTAgggg                                                                                                                                                     <  2:452621/251‑1 (MQ=255)                                                                                                  gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAc                                                                                                                                                   <  1:308400/251‑1 (MQ=255)                                                                                                  gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAc                                                                                                                                                   <  1:480911/251‑1 (MQ=255)                                                                                                              cGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGatac                                                                                                                                       <  1:435350/251‑4 (MQ=255)                                                                                                               gATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATAcc                                                                                                                                      >  1:308405/1‑251 (MQ=255)                                                                                                                  cGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGct                                                                                                                                                                                                     >  2:163711/1‑184 (MQ=255)                                                                                                                  cGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGct                                                                                                                                                                                                     <  1:163711/185‑2 (MQ=255)                                                                                                                  cGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCAGCTCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTCCTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGGGAAGAGCAACGGAGGGGGAGTTAATCGATACCGc                                                                                                                                    >  2:66488/1‑250 (MQ=255)                                                                                                                    cACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGGACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAAGTTAATCGATACCGCAAc                                                                                                                                 >  1:130788/1‑251 (MQ=255)                                                                                                                                        aaaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑Agactgccc                                                                                                            >  1:84127/1‑244 (MQ=255)                                                                                                                                        aaaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGgcgc                                                                                                            >  1:475867/1‑251 (MQ=255)                                                                                                                                         aaCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGAGCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGcgcg                                                                                                           <  1:267636/251‑1 (MQ=255)                                                                                                                                           cgttacGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGGTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGcgcggc                                                                                                         >  2:471028/6‑249 (MQ=255)                                                                                                                                                cGTGCCTAGCTGCGCGCCATCAACCCTGATCTTGCACCGATGAATACTCATCGCGGAAGGGGAGATAGCCTGAGGGGCCAGTAAAGCGTATCGGCATGCGGTTGCTGCTAGGCTATTTTCTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATGTGTGTCAAAGAAGTTAAGCCGGGCTTGAGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGAt                                                                                                    <  2:372759/251‑1 (MQ=255)                                                                                                                                                  tGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGAttt                                                                                                  >  1:208109/1‑251 (MQ=255)                                                                                                                                                    ccAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGCGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGGAGGGCAACGGAGGGGGCGTTAATCGATACCGCAAAGTTGCTGGCGG‑AGCAGGGGCGTCCCGCTTTgg                                                                                                >  2:243336/1‑250 (MQ=255)                                                                                                                                                               gcCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGAc                                                                                     >  1:347449/1‑251 (MQ=255)                                                                                                                                                                  aTCAACCCCGATCTTTCACCGATTAATCCTCATCGCGGCATGGGATATAGACTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTGGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAAGGCAACGGGGGGGAAGTTAAGGGCTACGTCAACGTGGCTGGGGG‑AGTTGGTGCGTCCCGATTTGccgtcttggggccc                                                                                   >  1:186254/1‑237 (MQ=255)                                                                                                                                                                  aTCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTTTTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCCACGGAGGGGATGTTAATCGATACCGCAACGTTGCTGGCGGGAG‑TGGCGCGTCCCGAttttgtccctgggggacc                                                                                   >  2:391755/1‑235 (MQ=255)                                                                                                                                                                           gATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGAATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTACGTACTGGCTATTTTTGTCAAAGAAGATAATCCGGGAGTGCGAAGAGCAAAGGAGGGGACGTTAATCGCTACCGCAAAGTTGCTGGCGG‑GACTGGCGCGTCCCGGTTTGCTCCCTGGGGAGCCAACgaccggg                                                                         >  2:430263/1‑245 (MQ=255)                                                                                                                                                                                         tAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTAATCGCTACCGCCACGTTGCTGGCGG‑AGCTGGGGCGTGCCAGTTTGCTCTCTGGGGACCCACCCCCTGGGCAAcaggggcgggg                                                           >  2:373982/1‑241 (MQ=255)                                                                                                                                                                                               tCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAc                                                                                >  1:411675/1‑224 (MQ=255)                                                                                                                                                                                               tCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAc                                                                                <  2:411675/224‑1 (MQ=255)                                                                                                                                                                                                   ggcgGCATGGGATATAGGCTGAGGGGGCTGTAATGCGTATCGGCATGCGGGTGCTGCTGGGCTATTTTTGTCTGGTGGCGGTGGCGGCCTGGTTCGTAATGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGATGGGACGTTAAGCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAg                                                 <  2:240606/250‑1 (MQ=255)                                                                                                                                                                                                         aTGGGATATAGCCTGAGGGGCCTGTAATGCGTAGCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTacaa                                           <  1:335164/251‑1 (MQ=255)                                                                                                                                                                                                           gggttataGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTacaac                                          <  2:347449/246‑1 (MQ=255)                                                                                                                                                                                                             gATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGTGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATc                                       <  1:331702/251‑1 (MQ=255)                                                                                                                                                                                                              atatAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑GGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCCAACGCCTGGGCAACTGGCGCAGGAGTTTAATCAACTAAAACATCg                                      >  1:164500/1‑251 (MQ=255)                                                                                                                                                                                                               tataGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGGAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCCAAGTTGCTGGCGG‑AGCTGGCGCGTCCCGCTTTGCTCTCTGGGGACCCAACCCATGGGGCACAGGCGCAGGCGTTTAATACGCTACAACATCGc                                     >  2:8785/1‑251 (MQ=255)                                                                                                                                                                                                                       gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTCCTGGCTATTTTTGTCCAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGATAATCGATACCGCAACGTTGCTGGCGG‑CGCCGGCGCGGCCCGAGTTGCTCTCTGGGGACCCAAACCATGGGCAACTGGCCCAGGCGGTTAATCCGCTTCAACAACGCCCGtttgc                             >  2:209857/1‑249 (MQ=255)                                                                                                                                                                                                                       gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTc                              <  1:180867/250‑1 (MQ=255)                                                                                                                                                                                                                                  aaTGCGTATCGGCATGCGGGTGCTGCTGGGCTATTTTTTACTGGTGGCGGTTGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTGGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCg                  <  1:66488/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGTCGTTAATCGCTACCGCACCGTTGCTGGCGG‑GGCTGGCGCGTTCCGATTTTCTCTCTTGGGGCCCCACCCCTGGGCAAATGGCGCAGGCGTTTTATCCGCTACCACCTCGCCCGTTTCCCGCCAATTTCTGTGGcctttaacatat  >  2:51265/1‑240 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGG‑AGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGT  >  NC_000913/4636470‑4636961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑