Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,543	G→A	L179L (CTG→CTA)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,543	0	G	A	100.0%	134.8 / NA	42	L179L (CTG→CTA)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/15);  total (27/15)

GCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGT  >  NC_000913/4636298‑4636784                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATgg                                                                                                                                                                                                                                               >  1:262699/1‑250 (MQ=255)          aatgtgacTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCGTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCCGCTGGTTTGACACGCCATTAACGCTGACGCGGAATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCTAGCCGCGTCTGGTCCCGCCAGCACCTAATGGCTAGCGtgtg                                                                                                                                                                                                                                     >  2:352122/8‑248 (MQ=255)                                cccGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGCAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGCGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGACGCGTCTGGTCCCGCCAGCCAATAATGGTTAGCGGGTGGGTAGAGGCGCAGGACCCCTAc                                                                                                                                                                                                               >  2:259117/1‑251 (MQ=255)                                          gtgCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCg                                                                                                                                                                                                     >  1:272823/1‑251 (MQ=255)                                           ggcgcCGGGGTGCGCCCCTTAGTGGGGCGGGTGAAGAAGTTCTGGCCGCGGGCCCCCGTCATCGGTTTGGGTCATTTTGAATTGAATGACCCCGCGGCGAAGATAGGCGGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGt                                                                                                                                                                                                    <  1:482565/250‑1 (MQ=255)                                                               aCTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGa                                                                                                                                                                                                                               >  2:337048/1‑204 (MQ=255)                                                                               aagTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCg                                                                                                                                                                                                          >  2:482331/1‑209 (MQ=255)                                                                               aagTTCTCGCCGCCGTCCCCCGTCACCCGTATTGGTCATTTTGAATTGAAGGACCCCGCGGCGCAGATCAGCTGGTTGGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCg                                                                                                                                                                                                          <  1:482331/209‑1 (MQ=255)                                                                                 gTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCAAAGCTgcgcg                                                                                                                                                              >  1:79054/1‑251 (MQ=255)                                                                                   tctcGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGCTCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTATGGTCCTGCCAGCAACTAATGGATAGCGTGTGGGAGGATGCGCAGGCCACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGcc                                                                                                                                                            >  2:75592/1‑251 (MQ=255)                                                                                             gTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTACTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCACGCTGCGCGCCATCACccccc                                                                                                                                                  >  2:180867/1‑250 (MQ=255)                                                                                             gTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGCTACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCg                                                                                                                                                  >  2:308400/1‑251 (MQ=255)                                                                                                tcCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGAGCAGATCAGCTGGTTTGACACGCAATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGAACCGTTGATACCAACATTAAAAAGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATc                                                                                                                                               >  1:155630/1‑251 (MQ=255)                                                                                                    gTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTc                                                                                                                                           >  2:116036/1‑251 (MQ=255)                                                                                                      cATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGCTCTTTCAc                                                                                                                                         >  2:366035/1‑251 (MQ=255)                                                                                                                     ttttGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATc                                                                                                                          >  1:70199/1‑251 (MQ=255)                                                                                                                                    ccccggggtgaagatctgttGGTTTGACACGCCATAAACGCTGACGCGGTATGATTTTTTATTGCTGAAGACGTTACCCAAGTCGCCAGGCCGCGTCTGGGCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGAtata                                                                                                           <  2:70199/232‑1 (MQ=255)                                                                                                                                         cggcgCAGGACAGCTGGTTTGACACGCCATTAAAGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGACCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGAGCACTCAACCCCGCTCTTTAACCGATTAATACTCCTCGCGGCATGGGAGATAGccat                                                                                                      >  2:474038/1‑249 (MQ=255)                                                                                                                                         cggcgCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGtt                                                                                                                                                                                                   >  2:411866/1‑157 (MQ=255)                                                                                                                                         cggcgCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGtt                                                                                                                                                                                                   <  1:411866/158‑2 (MQ=255)                                                                                                                                               ataTCAGTTGGTTTGACACGCCATTAACGTTGACGCGGTATGAGTTTTTAGTGCTGAAGACGTTACTCAAGTCGCCAGGCTGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGc                                                                                                <  1:363699/249‑1 (MQ=255)                                                                                                                                                   cggttgttttGACCCGCCATTACCGTTGACGGGGTAGGATTTTTTATTGCTGAAGCCTTTACCCAAGCCGCCAGGCCGCGTTTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGAGCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                                                                            <  2:452968/244‑1 (MQ=255)                                                                                                                                                   cAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCCTCTCGGCCTGGGATATAGCCTGAGGGGCCTGt                                                                                            >  2:228925/1‑251 (MQ=255)                                                                                                                                                           ttGACACGCCATTAACGTTGTCGCGGTATGAGTTTTAATTGCGGAAGACGTTCCTCAGGTCGCCAGTCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGt                                                                                     <  1:36137/250‑1 (MQ=255)                                                                                                                                                             gcaacGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCAGAAGCTGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATc                                                                                  <  1:176256/248‑1 (MQ=255)                                                                                                                                                             aacactccattaacgcggatgcGGTATGAGTTTTTTGTGCGGAAGCCGTTACTCAAGGCGCCAGGCCGCGTCTGTTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACTCCTACGATCGCACTGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTAt                                                                                   <  1:301728/229‑1 (MQ=255)                                                                                                                                                                             tGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATAGCGGCATGGGATATAGCCTGCGGGCACGGTAATGCGTATCGGACTGCGGTtgctgc                                                                  >  2:267636/1‑251 (MQ=255)                                                                                                                                                                               acgcGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                     >  1:221834/4‑150 (MQ=255)                                                                                                                                                                               acgcGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCGGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                     <  2:221834/147‑1 (MQ=255)                                                                                                                                                                                      aTGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGCTATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtt                                                         >  2:480911/1‑251 (MQ=255)                                                                                                                                                                                        gAGTTTTTATTGCTGAAGACGGTACCCAAGTCGCCAGTCGGGGTCGTGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATTCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtttt                                                       <  1:30569/251‑1 (MQ=255)                                                                                                                                                                                          gTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGCAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGAATCGGCATGCGGTTGCTGCTGGGCTAtttttt                                                     >  1:452621/1‑251 (MQ=255)                                                                                                                                                                                          gTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCgg                                                                                >  1:40646/1‑224 (MQ=255)                                                                                                                                                                                          gTTTTTATTGCTGAAGACGTTACTAAAGTAGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtttttt                                                     >  1:394760/1‑251 (MQ=255)                                                                                                                                                                                           tttttATTGCTGAAGGCGTTCCTCAAGGAGCCCTGCCGCGACTGGTCCAGCCAGCAACTAATGGCTAGCGAGTGGGAAGATGCGCAGGACACCTACGATCGCAACGTTGATAACCACATTAAAACGGTACGTGCCAAGCTGAGCGGCATCAACCCCCATCTTTCACCGATTAATACTCATCGCGGCATGGGATAAAGCCTGGAGGGACTGAAATTCGGACCGGAATGCGGTGGCTGCATGGCTAtttttgg                                                    >  2:325083/1‑249 (MQ=255)                                                                                                                                                                                                           cGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcggtggcgg                                    >  1:385251/1‑249 (MQ=255)                                                                                                                                                                                                                  cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATACGGTTGCTGCTGGGCTATTTTTTATTggtggaggtggcggaccgggt                             >  2:381647/1‑242 (MQ=255)                                                                                                                                                                                                                       ccccgggcccgttCTGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGAGCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTAc                        <  2:385251/239‑1 (MQ=255)                                                                                                                                                                                                                              ccgcgcttggccccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGctat                 <  1:12347/240‑4 (MQ=255)                                                                                                                                                                                                                                         cccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCaa        >  1:330803/1‑249 (MQ=255)                                                                                                                                                                                                                                            gCCAGCAACTAATGGATTGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATCCTTATCGCGGCAGGGGATATAGCCTGAGTGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAaagaag   <  2:290268/251‑1 (MQ=255)                                                                                                                                                                                                                                             ccAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGt  <  1:228925/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGT  >  NC_000913/4636298‑4636784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑