Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,658	T→C	D46D (GAT→GAC)	creA →	PF05981 family protein CreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,658	0	T	C	100.0%	96.6 / NA	33	D46D (GAT→GAC)	creA	PF05981 family protein CreA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

AGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCT  >  NC_000913/4635419‑4635906                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                         aGGGCTTTACGGAAATAAAAGTAGTGAGATTTTGTTCTGAATCAAAATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATTGTAAAAATATGAAATACAAGCATTGGATCCTGTCGTTAAGTCTGATAATGCTGGGGCCATTGGCGCATGCAGAAGAGATTGGTTCGGTCGACACCGGATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTTGAAGCCGTTGACGATCCAGAtgt                                                                                                                                                                                                                                               <  2:301783/251‑1 (MQ=255)        gACGGAAATAAAAGTAGTGAGATTTTGTTCTGAATCAATATGTTAGTTACCGTGACGAACTAATTGCGCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGGCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGAGCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAAt                                                                                                                                                                                                                                        <  2:241062/251‑1 (MQ=255)                   aaggaTTGAAATTTTGTTCTTAGTCAATCTGTTATTTACCGTGTCGAACTCCTTGCTCGTGTACTAGATAAAATTGGTAACAATCTGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGCTAATGCTGGGGCCATAGGCTCATGCCGACGCGATTGGTTCGGTCGACCCCGTATTTAAACTGATCGGCCCGGATCACAAAATT‑GTTTTTGCAGCCTTTGACGTTCCCGATCTGAAAAATGTCTCCTGttt                                                                                                                                                                                                                             >  2:485403/5‑250 (MQ=255)                       aTTGAGATTTTGTCCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATCAAACTGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAACTGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGCCGTTCCCGATGGGAAAAATGTCACCTGTTAtgtg                                                                                                                                                                                                                         >  2:31057/1‑251 (MQ=255)                                           cAATATGTTATTTACAGTGACGAGCTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACAGTATTTAAAATGCTCGGACAGGATCACAAAATT‑GTTGTGGAAACCTTTGCCGATCCCGATGGGAAAAATGTCAACTGTTATGTACGCGGGGCGAAAAACggtgg                                                                                                                                                                                                     >  2:186682/1‑251 (MQ=255)                                               atGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTAtt                                                                                                                                                                                                 >  2:317383/1‑251 (MQ=255)                                               atGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTAt                                                                                                                                                                                                  <  1:428036/250‑1 (MQ=255)                                               atGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAAGATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGTTGGTAt                                                                                                                                                                                                  <  2:303382/250‑1 (MQ=255)                                                           gTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGGTGTGAAAAATGTCACCTGTTATGTGGGCCGGGCGAAAACAGGTGGTATTAAAGGGGGAtcg                                                                                                                                                                                     >  2:221698/1‑249 (MQ=255)                                                                gAACTAATTTGTCTTGTAACAGATAAAAATGGTAACAATATAAAATGCAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGTCCAGTGGCTCATTCAGAAGAGATTGGTTCGGTCGACTCCGGATTTAAAATGATCGGCCCTGATCACAAAATT‑GTTGTGGAGGCCTTTGTCGATCCTGATTTGAAAAATGTCGCCTGGTATGTCTGCCGTGCGAAAATAGTTGGTATGAAAGAGTGATTGGGTCt                                                                                                                                                                                <  2:473917/251‑1 (MQ=255)                                                                 aaCTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTg                                                                                                                                                                               <  1:114570/251‑1 (MQ=255)                                                                 aaCTAATTGCTCGTGGAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTg                                                                                                                                                                               <  2:87778/251‑1 (MQ=255)                                                                                 aaTAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCg                                                                                                                                                               >  1:15686/1‑251 (MQ=255)                                                                                 aaTAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTAGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCAGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCg                                                                                                                                                               >  1:466636/1‑251 (MQ=255)                                                                                            aTGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGCTGCGGCTAtt                                                                                                                                                    >  1:321813/1‑251 (MQ=255)                                                                                               gTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGACCTTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGACGATACCTACGATGCGGctttttgt                                                                                                                                                 >  2:64416/1‑244 (MQ=255)                                                                                                   cAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTCAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTc                                                                                                                                             >  1:443460/1‑251 (MQ=255)                                                                                                         gAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCGCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGTTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAg                                                                                                                                       <  2:141413/251‑1 (MQ=255)                                                                                                                    aTTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTc                                                                                                                                             <  2:238206/234‑1 (MQ=255)                                                                                                                    aTTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTc                                                                                                                                             >  1:238206/1‑234 (MQ=255)                                                                                                                                                               tCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAgg                                                                                 >  1:99261/1‑251 (MQ=255)                                                                                                                                                                      gaagaGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCg                                                                                                                                                                            <  2:157659/153‑1 (MQ=255)                                                                                                                                                                      gaagaGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCg                                                                                                                                                                            >  1:157659/1‑153 (MQ=255)                                                                                                                                                                                               tATTTAAAATGATCGGCCCGGATCACAAATTT‑GTTGTGGCAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCACCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCAGATTGAACTGGAGGAACGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAATATTCAAATAACGCACGGCCCTGGTCttt                                                 >  1:478109/1‑251 (MQ=255)                                                                                                                                                                                                aTTTAAAATGATCGGCCCGGCTCACAAAATT‑GTTGTGGAAGCCTTTAACGCTCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAGCAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGCTACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGGCGGATCGTATTATAAACGGCAAAGCACAGGGCGCGGAAGTATTCAAAAACCGCACGACCCTGGCCttt                                                 >  2:92319/1‑250 (MQ=255)                                                                                                                                                                                                aTTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGa                                                                                                                                                              >  2:91146/1‑141 (MQ=255)                                                                                                                                                                                                aTTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGa                                                                                                                                                              <  1:91146/141‑1 (MQ=255)                                                                                                                                                                                                     aaaTGATCGGACCGGAACACAAAATTTGTTG‑GGAAGCATTTGACGATCCCGATGTGAAAAATGGCCCCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCg                                           <  2:310169/251‑1 (MQ=255)                                                                                                                                                                                                                      cacaAAATT‑GTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCtt                          <  1:402848/251‑1 (MQ=255)                                                                                                                                                                                                                                gttgtGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCCCCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTatgacg                  <  1:221698/250‑3 (MQ=255)                                                                                                                                                                                                                                        aGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAaacgca         <  1:430948/251‑1 (MQ=255)                                                                                                                                                                                                                                          ccTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTGCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAaacgcaac       <  2:466636/251‑1 (MQ=255)                                                                                                                                                                                                                                            tttGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTCCAGGTCGTGCGCTTTTAAGACGCAAaacgcaccgc     >  2:466181/1‑251 (MQ=255)                                                                                                                                                                                                                                               gatgatCCCCATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGcgct  >  1:258327/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                         AGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATT‑GTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCT  >  NC_000913/4635419‑4635906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑