Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,132	A→C	G276G (GGT→GGG)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,132	0	A	C	100.0%	93.9 / NA	31	G276G (GGT→GGG)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/9);  total (22/9)

AGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAA‑GCCCTGCATGAGCCGGGCGAC  >  NC_000913/6899‑7362                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        aGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGACTACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCCGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGAAG‑CGGGGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCAtt                                                                                                                                                                                                                        >  2:310811/1‑251 (MQ=255)   ggTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTgg                                                                                                                                                                                                                      >  1:60338/1‑251 (MQ=255)     tAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCCGGCCGCCGCTGCC‑GCTGCCGAGTTTGGCGTCGACCCCATTCCCGCCTCATTggc                                                                                                                                                                                                                     >  1:474166/1‑249 (MQ=255)                       aGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGaaa                                                                                                                                                                                                   >  1:61678/1‑250 (MQ=255)                             ccATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGtt                                                                                                                                                                                             >  1:272461/1‑250 (MQ=255)                                   acACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGAAATc                                                                                                                                                                                      >  1:204770/2‑251 (MQ=255)                                              gCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGAAAGTAATATCAGCATGGCGCTTGCAGTACCGATGACCAGGGTGTCGATAAAAATGCCAATCATATGGACAATCCCTTGCGCTGCCGGATA‑CGGCAGCCAGGACGACGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATAATGCGCTGAAAACCGGTAATAATCGCCTGACTTa                                                                                                                                                                            >  1:281363/1‑250 (MQ=255)                                                                   ccTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTAAAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGAAG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTAGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGAATATACAGCCGCgccgcc                                                                                                                                                      >  1:319848/1‑251 (MQ=255)                                                                            ggCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCt                                                                                                                                                     >  1:420252/1‑243 (MQ=255)                                                                                 gTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTgcc                                                                                                                                        >  1:3472/1‑251 (MQ=255)                                                                                       tgtgACGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGCCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACAGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCGGCCGCTCCCTgcaagcca                                                                                                                                   >  2:410861/1‑250 (MQ=255)                                                                                                        gTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAAt                                                                                                                  >  2:394854/1‑250 (MQ=255)                                                                                                             ataaGCATGGCGCTTGCCGTACAGATGCCCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGCCCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGAcc                                                                                                             <  1:152902/247‑1 (MQ=255)                                                                                                             aacAGCATGTCGCTTGCCGTACACATGCCCAGGGGGCCGATAAAAATGCCAATCATCTGGAAAATCCTTTGCGCTGCCGGAGG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGCGGCCCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGAcc                                                                                                             <  1:433135/248‑1 (MQ=255)                                                                                                                               gaaaggaggacaggggTGTCGAAAAAACGGCAAATAATCGGTACAATCCCGTGCGC‑GGCGGAGGGCGGGGGCCAGGACGCGGCGGCC‑GCGTCCGCGTTTGGCGCCGCCCCCATCCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTg                                                                                          <  2:70481/238‑1 (MQ=255)                                                                                                                                      tGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAACTGACGTGGGGAAGTTGCCCGat                                                                                    >  2:373523/1‑250 (MQ=255)                                                                                                                                                    aTAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAAt                                                                     >  1:146066/1‑251 (MQ=255)                                                                                                                                                                        gACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCACCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTAc                                                                           >  1:240761/1‑225 (MQ=255)                                                                                                                                                                         ccagccccgtGCGCGGCCGGAGG‑CGGAGCCCGGGCCCCCGCGCCC‑GCTGCGGCGTTGGGGGCGGACCCCATTCCCGCCTCATGGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCa                                                <  2:237657/242‑1 (MQ=255)                                                                                                                                                                          cAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGTCGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGAAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGCCCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAACTCCACAGGCAGGTCAGTAccc                                                 >  2:247740/1‑249 (MQ=255)                                                                                                                                                                           aaTCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGCCAGTAccc                                                 >  2:360331/1‑248 (MQ=255)                                                                                                                                                                                      cgcccGGAGG‑CGGAGGCCGGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATc                                   <  1:360331/248‑1 (MQ=255)                                                                                                                                                                                            gATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCCGTACCCAGATTATCGCCATCAACggg                             >  1:93977/1‑251 (MQ=255)                                                                                                                                                                                             aTG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACggg                             >  2:93021/1‑250 (MQ=255)                                                                                                                                                                                               gggggagcccggtcgcccgcgccc‑gcggccgcTTTTGGCGTCGTCCCCTCCCCCGCCTCATTGAAAAACATACTGCGCTGAAAACCGTTAGTAAGCGCCTGGCTAAAGGTATACCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGAc                           <  2:51707/229‑1 (MQ=255)                                                                                                                                                                                                              cccccgccc‑gcggccggGTTGGGCGTCCACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAA‑CCCCTGCATGAg           <  2:146066/244‑1 (MQ=255)                                                                                                                                                                                                                cgctgcccgctgccGGTTTTGGCGCCCACCCCTTCCCCGCCTCATTGGAAAACATACTGCGCGGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAA‑CCCCTGCATGa            <  2:61678/249‑1 (MQ=255)                                                                                                                                                                                                                    gcc‑gctgccgcGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAA‑CCCCTGCATGAGCCGGGc     >  1:331976/1‑251 (MQ=255)                                                                                                                                                                                                                    gcc‑gctgccgcGTTGGGCGTCGCCCCCATCCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAA‑CCCCTGCATGAGCCggg      <  1:245919/250‑1 (MQ=255)                                                                                                                                                                                                                        gatgccgcGTTTGGCGACGGCCCCATTCCCGACTCATTGTAAAACATCCTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGGATATACCGCCGCGCCGACTGCCGCTTCCGGCCAGCCAAAAGCACGCTCAAAAATCGCCCCAATGACGTGAGGAAGTTGCCCGATATTAATTACGCAACTCACCCGGCAGGACCGTACCCAGATTACCGCCAACCACGG‑ACAAAACCCCTGCATGAGACGGGCGAc  >  2:139540/3‑251 (MQ=255)                                                                                                                                                                                                                                gTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCa                                                                >  2:75047/1‑182 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        AGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATG‑CGGAGGCCAGGACGCCGCTGCC‑GCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAA‑GCCCTGCATGAGCCGGGCGAC  >  NC_000913/6899‑7362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑