Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,553	A→G	intergenic (‑31/‑180)	ettA ← / → slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,553	0	A	G	100.0%	129.0 / NA	40	intergenic (‑31/‑180)	ettA/slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/18);  total (22/18)

CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTC‑GATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGC  >  NC_000913/4630321‑4630798                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       gtGGCTGCGGACCCTCTTCCCCTCCATTGTCTTTAACAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGa                                                                                                                                                                                                                                      <  1:408408/250‑1 (MQ=255)       ccggacggtctccccctttgtggtttttATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTa                                                                                                                                                                                                                                <  1:77060/226‑1 (MQ=255)                 ttCACCTTCGATGTCATTATCAATGCCGGCCATAATGCTCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGACAAACTTTGCCGCCACGGTGCATGGTATAAACGAATTGAACCACGTTGGACTTCGCCTCTATGTTTGTCGTGATGGTGGGTTTGCCAAGGCGAAGTGTAGACTTTTTcc                                                                                                                                                                                                                      >  2:424549/1‑251 (MQ=255)                            tGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCCACGGCGAAGTGTAGCCTTTTTCCCCGCCTAATcc                                                                                                                                                                                                           >  2:155486/1‑251 (MQ=255)                                             gCCAAAATGCGCAGCAGGGTAGACTTACCCGCTCCGGTCAGACCCAGTACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGTTATAAACGAAGTGAGCCACGTTGGACTTTGCCTCTATGTTTTCCGTGATGATGAGTTTTCAAAGGTGAAGTGTAGTCTTTTTCCCCTCCTAATCCCAGTTGGTC‑GATcgcct                                                                                                                                                                                         <  1:396473/251‑5 (MQ=255)                                                aTAAGGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCg                                                                                                                                                                                      <  1:258619/251‑1 (MQ=255)                                                                  gACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACATTTCGGCGGAACAACTTTTCCGACACGATGCATGGTATAAACGGATTGATCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGAGAAGCCTTTTTCCCCGCCTAATCCCATTTGGTC‑GATCACCTTCGCcgtaaaagtactaca                                                                                                                                                                      >  2:118937/1‑244 (MQ=255)                                                                           gcgcCGTTCAGACCCAGGACACCAATTTTGGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAAc                                                                                                                                                           <  1:155486/251‑1 (MQ=255)                                                                               cgttCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTgg                                                                                                                                                       <  1:156911/249‑1 (MQ=255)                                                                                                       ttGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAg                                                                                                                                <  1:258384/250‑1 (MQ=255)                                                                                                         gCCCCAGGGAAGAAACTCAAAGAGATGTTTTGCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCAt                                                                                                                             <  2:407691/251‑1 (MQ=255)                                                                                                          ccccAGGGCAGACACTCAGAGAGATGTTTTTCAAAATATGACGCTTCGGCGGAACAAATTTGCCGACACGATGCATGGTATAAACGAATTGTGTCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGGGTTTTCAACGGCGAAGTGTAGCCTTTTTCCCCGCCTATTCCCAGTTGGTC‑GCTCACCTTCGCAGTAGATGTAAAAAAATATACGTAATGTTGCGTAAACGGCatttattggctcaaat                                                                                                                             >  2:342286/1‑235 (MQ=255)                                                                                                              aGGGAAGAAACTCAGAAAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACATTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGAATTCGCCTCTATGTTTATCGTGATGATGAGTTTTCCAAGGCGAAGTGTAGCCTTTTTCCACGCATAATCCCAGTTGGTCTGA‑AAGCTTCGCAGTAAAAGTCAAAACGAGACCGTAACGGGGAGTAAACGGCAATGCCGGGATAGCATAAAtt                                                                                                                        >  2:40548/1‑250 (MQ=255)                                                                                                                      aaCTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAcgc                                                                                                                >  1:231723/1‑251 (MQ=255)                                                                                                                           agagagATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCAt                                                                                                           >  1:369971/1‑251 (MQ=255)                                                                                                                           agagagATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCAt                                                                                                           <  1:228829/251‑1 (MQ=255)                                                                                                                            gtgagaTGTATTTCAAAAAATTCCGTTTCGGCGGCACCAATTGGCCCGCACGAGGCATGGTATAAACTAATTGAGCCACTTTGGTCTTCGCCTTTATGTTTATCGTGAGGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGGC‑GATCAACGTCGCATTAAAAGTAAAAAAGTTTCCGTAACGTCGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTTCGCGGCAtt                                                                                                          <  2:281871/249‑2 (MQ=255)                                                                                                                               ggaTGTTTTTCAAAATAGGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACg                                                                                                       <  2:125254/250‑1 (MQ=255)                                                                                                                                 aTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGa                                                                                                                                        >  1:360369/1‑216 (MQ=255)                                                                                                                                 aTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGAATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGa                                                                                                                                        <  2:360369/216‑1 (MQ=255)                                                                                                                                             atatGACGTTTCGGCGGAATCTCTTGGCCGACTCGATGCATGGTACAATCGAATGTAGCCACGGTGGACTTTGCCTCTATGTTTTTCGTGAAGATGAGTTTTCAAAGGCGAAGTTTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTGCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGTCGCTGCATTGATGTAt                                                                                         <  2:406708/251‑1 (MQ=255)                                                                                                                                                     tttCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCg                                                                                                                                                                                                                                       <  2:378419/102‑1 (MQ=255)                                                                                                                                                     tttCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCg                                                                                                                                                                                                                                       >  1:378419/1‑102 (MQ=255)                                                                                                                                                      ttCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGCATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACtt                                                                                 >  2:41574/1‑250 (MQ=255)                                                                                                                                                      tcgggcggaaatactgggCCGACACGATGCATGGGATAAACGAATTGAGCCACGTTGGACTGCGCCTCTATGTTTATCGGGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTATCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACtt                                                                                 <  2:436644/233‑1 (MQ=255)                                                                                                                                                             gAACAACTTTGCCGACACGATGCATGGTATAAACGAATTAAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGCTGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCATCGCAGTAAAAGGAAAAAAGAGTCCGTAACGTGGCGTAAACGGCAGTGAATGGTTAGCATAAATCTATTACGCGGAATGACGCTGCATTGATGTATTTACACTTAAAGGATg                                                                         >  2:121352/1‑251 (MQ=255)                                                                                                                                                                caacTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCAccttc                                                                                                                                                                                       <  2:264334/138‑4 (MQ=255)                                                                                                                                                                caacTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCAccttc                                                                                                                                                                                       >  1:264334/1‑135 (MQ=255)                                                                                                                                                                    tttGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTtgt                                                                  >  1:474894/1‑251 (MQ=255)                                                                                                                                                                         cGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaa                                                              >  1:222181/1‑250 (MQ=255)                                                                                                                                                                           acacGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaaaa                                                            >  2:265696/1‑250 (MQ=255)                                                                                                                                                                                 tGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTACCGTGGCGTAACCGGCAATGACTGGTTAGCATAAATCTATTACGCGGCCTGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCaa                                                      >  2:287415/1‑250 (MQ=255)                                                                                                                                                                                             aCGAATTGAGCCACGTTGGACTTCGCATCTATGTTTATCGTGATGATGAGGTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGAATTTATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTgg                                         >  2:283020/1‑251 (MQ=255)                                                                                                                                                                                                              ggccTTCGCCTCTATGTTTATCGTGATGATGAGGTTTCAAAGGCGAAGTGGTGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCg                         <  2:51386/247‑1 (MQ=255)                                                                                                                                                                                                                     gCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAAAGGCAATGACTGGTTAGCATAAATCTATTAAGCGGCATGACGCTGCATTGCTGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGtgtctgt                 >  1:206177/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGtgtctgtc                >  1:334330/1‑251 (MQ=255)                                                                                                                                                                                                                            tGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGGGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTACAAGTAAAAACGTGTCCGTAACGTGGCGTAACCGGCCATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGATTGTGGAAAAAGCCAAACAAGTTACATGGCGGCTGTTGGCTGCCGGTGTCTGTctgcagc          >  2:292907/1‑248 (MQ=255)                                                                                                                                                                                                                                 aTCGTGATGATGAGTTTTCAAAGGCGAAGGGGAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGt      <  2:128301/250‑1 (MQ=255)                                                                                                                                                                                                                                 aTCGTGATGATGAGTTGTCAAAGGCGCAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGAGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCCTTGATGTATTTACACTTAGCGGATGAGCTTGTGGAAAAAGCCAAACAAGATACCTAGAGGCTGTTGGCTGCCGGAGTCTGTCTGATGACGGTc     >  1:352586/1‑251 (MQ=255)                                                                                                                                                                                                                                    gTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTC‑GATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTcagc  >  1:420723/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTC‑GATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGC  >  NC_000913/4630321‑4630798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑