Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,385 G→A S46S (TCC→TCT)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,3850GA89.7% 69.1 / ‑1.2 30S46S (TCC→TCT) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (17/9);  total (20/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

GGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCG  >  NC_000913/4630157‑4630618
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
ggCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGGAACCAATCTTGATGT‑ATGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATCATGCACATCCGGGAGTACTTTCCCGCGCCCTTCAGa                                                                                                                                                                                                                      >  2:258830/1‑250 (MQ=255)
 gCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCGGCGGGCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGAcc                                                                                                                                                                                                                    >  2:464474/1‑251 (MQ=255)
         gTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGTTGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGGAGACTTACCcgcg                                                                                                                                                                                                                               >  2:362126/1‑232 (MQ=255)
            aaaaTCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGAAGACTTACCCGCGCCGTTCAGACCCAggcaacca                                                                                                                                                                                                          >  1:171901/1‑244 (MQ=255)
                            tCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGCCTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCTGACCCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATCATACACAGCAGGGACGACTTACCCGCGCCGTTACTACACAGGACACCCATTTTTGCCCCAgtga                                                                                                                                                                                          >  2:336224/1‑247 (MQ=255)
                             cGGCGTACAGCGCCTACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTCACCCATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCCGCAGGGTAGACTTAACCGCGCCGGTCCGACCCAGGACACCAATTTTTTCCCCAGGga                                                                                                                                                                                          >  2:462728/1‑249 (MQ=255)
                                       cgcATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCACTTTTTGCCCCAGGGAAGAAACTCag                                                                                                                                                                                >  2:372613/1‑249 (MQ=255)
                                            acacTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGCCACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATg                                                                                                                                                                         >  1:111421/1‑251 (MQ=255)
                                                      cAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGCTGTTTTTCaaa                                                                                                                                                                >  1:125254/1‑250 (MQ=255)
                                                        ggCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTATTCAATGGACTCACGAACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTGACCTTCGATGTCTTTATCAATGCCCGCCCTAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCACCAAGGACGGAAATCAGAGAGAAGTTTTTCAAAat                                                                                                                                                              >  1:420430/1‑250 (MQ=255)
                                                           gTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCCTAATGCGCAGCCGGGGAGACTTACCCGCGCCGTTCAGACCCAGGACCCCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATg                                                                                                                                                           >  2:258384/1‑250 (MQ=255)
                                                            tttCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTTTCAATGCCCGCCATCATGCGCCGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGCCACCAATTTTTGCCCCAGGGAAAAAACTCAGCGGGATGTTTTTCAAACTATGAc                                                                                                                                                         >  2:156911/1‑251 (MQ=255)
                                                               gggggcttaaccccctacaaaaaCCGCTTCTGCAATGGACCCACGCACGGTGTGTTC‑GGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCGGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGtt                                                                                                                                                      <  1:395397/231‑1 (MQ=255)
                                                                   gCGTTAACCACTTCAGAACCCGCTTCTTAAAGGGCCCCACGCGCGGTGTGTCC‑CGGGTTCAGCTGGGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CGGGCGGCGGACGCGCTTCCCCTTCGATGTCTTTACCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcgg                                                                                                                                                  <  1:76546/251‑1 (MQ=255)
                                                                           cACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTAC‑CGGGTTCAGCTGCGGTTCATGCGGCAGGCAAACAATCTTTA‑GTGCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTTTCAATGCCCGCCATAATGGGCATCAGGGTAGACTTACCCGAGCCCTTCCGGCCCCGGAAACCAATTTTTGGCCCAAGGAAGAAAATCTGAGAGATGGTTTTCAAAATATGGCGtttacgccgaaat                                                                                                                                           >  2:365673/1‑240 (MQ=255)
                                                                                           tcttcAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGGAGACTTACCCGCGCCGTTCCGACCCCGGACACCAATTTTTGCCCCCGGgaagaacct                                                                                                                                                                                   >  2:171918/1‑191 (MQ=255)
                                                                                             ttcAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGTTGT‑CTGGCTGCGGACGCGCTTCCCCTTCGTTGTCTTTTTCCATGCCCGCCATAATGCGCCACAGGGTAGACTTACCCGCGCCGTTCAGACCCCGGCCACCAAATTTTGCCCCAGGGAAGAAACTCAGCGAGATGTTTTTCCAAATATGACGTTTCGGCGGACCAACTTTGccaacccgctgcc                                                                                                                        >  2:316886/1‑240 (MQ=255)
                                                                                              cgaaTGGCCTCACGC‑TGGTGTGGTCGCGGGTTCAGCTGCGGTCCCGGCGGCAGATCACCATTTTTGATTT‑GTGGCGTCGGACGCGCTCCACCTGCGATGTCTTTAGCATTGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCAt                                                                                                                       <  1:316886/249‑1 (MQ=255)
                                                                                                    gcctcccgcggggTGTTTTC‑CGTGTTCGGCTGCGTTTCCTGCGGCAGGTAACCATTTTTGATGT‑CTGGCTGCGGACTCGCTTCTCCTCCGATGTTTTTAGCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGtata                                                                                                                 <  1:431708/240‑1 (MQ=255)
                                                                                                       tCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGTTTCTTGCGGCAGATACCCAATTTTGATTT‑CTGGCTGCGGACGCGCTTCCCCTTCGATTTTTTTATCAATGCCCGCCATAATGCGCAGC‑GGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAAc                                                                                                                                                                                    <  1:198129/180‑1 (MQ=255)
                                                                                                       tCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGTTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGC‑GGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAAc                                                                                                                                                                                    >  2:198129/1‑180 (MQ=255)
                                                                                                                     ttC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATACCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAACCACGt                                                                                                >  1:291230/1‑251 (MQ=255)
                                                                                                                           ggTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCcgc                                                                                                                                                                                                                                >  2:55289/1‑118 (MQ=255)
                                                                                                                               cggCTGCGGTTCCGGCGGCAGGTAACCAATTTTGATGT‑CTGGCTGCGGCCGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCg                                                                                       <  1:372613/249‑1 (MQ=255)
                                                                                                                                                                      gtGGCTGCGGACCCTCTTCCCCTCCATTGTCTTTAACAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGa                                                 <  1:408408/250‑1 (MQ=255)
                                                                                                                                                                            ccggacggtctccccctttgtggtttttATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTa                                           <  1:77060/226‑1 (MQ=255)
                                                                                                                                                                                      ttCACCTTCGATGTCATTATCAATGCCGGCCATAATGCTCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGACAAACTTTGCCGCCACGGTGCATGGTATAAACGAATTGAACCACGTTGGACTTCGCCTCTATGTTTGTCGTGATGGTGGGTTTGCCAAGGCGAAGTGTAGACTTTTTcc                                 >  2:424549/1‑251 (MQ=255)
                                                                                                                                                                                                 tGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCCACGGCGAAGTGTAGCCTTTTTCCCCGCCTAATcc                      >  2:155486/1‑251 (MQ=255)
                                                                                                                                                                                                                  gCCAAAATGCGCAGCAGGGTAGACTTACCCGCTCCGGTCAGACCCAGTACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGTTATAAACGAAGTGAGCCACGTTGGACTTTGCCTCTATGTTTTCCGTGATGATGAGTTTTCAAAGGTGAAGTGTAGTCTTTTTCCCCTCCTAATCCCAGTTGGTCGATcgcct     <  1:396473/251‑5 (MQ=255)
                                                                                                                                                                                                                     aTAAGGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCg  <  1:258619/251‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
GGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTC‑CGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCG  >  NC_000913/4630157‑4630618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑