Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 6,850 A→G Y370Y (TAT→TAC)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009136,8500AG96.7% 83.2 / ‑4.2 30Y370Y (TAT→TAC) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (16/13);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01

CGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGG  >  NC_000913/6607‑7092
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
cgggccaaagCCGAGTTTACGCTGGCGTAGATAATCACGGGCAAGGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGt                                                                                                                                                                                                                                              <  2:119746/242‑1 (MQ=255)
      cACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACAACAGCGGAAGACTTAGCCAGGGGCCGCCGATGAACGTTGCGGAGGGGCAGATCCGCAAACACCAGATCGCCTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGGAAATGTaatttg                                                                                                                                                                                                                                        >  2:223689/1‑246 (MQ=255)
                                 aTCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGaa                                                                                                                                                                                                             <  1:166966/251‑1 (MQ=255)
                                  acACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAAt                                                                                                                                                                                                            <  1:103847/250‑1 (MQ=255)
                                        gCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGGGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTGCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACcac                                                                                                                                                                                                      <  1:221274/251‑1 (MQ=255)
                                                     ccACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCCGGCCATTATGATAACTGCCAGTTGCCACCTCAGCGGAAGACTTAGCCAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTTAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGTCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAA‑AAAc                                                                                                                                                                                        >  2:119273/1‑251 (MQ=255)
                                                                                       gggaaTAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATc                                                                                                                                                       <  2:143890/247‑1 (MQ=255)
                                                                                          aaTAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCt                                                                                                                                                   <  2:341198/251‑1 (MQ=255)
                                                                                              gCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTAGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTg                                                                                                                                               <  1:47182/251‑1 (MQ=255)
                                                                                                    cAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATa                                                                                                                                           >  2:239314/1‑249 (MQ=255)
                                                                                                                      gCCAGTTGCCACATTAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTGCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCgg                                                                                                                       <  2:134692/251‑1 (MQ=255)
                                                                                                                          gTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATg                                                                                                                   >  1:70481/1‑251 (MQ=255)
                                                                                                                              ccACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAACCAGAATAACCACGAGGGAAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAgg                                                                                                               >  2:152902/1‑251 (MQ=255)
                                                                                                                                acaTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGtt                                                                                                             <  1:239314/251‑1 (MQ=255)
                                                                                                                                acaTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGtt                                                                                                             >  1:239512/1‑251 (MQ=255)
                                                                                                                                      gCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAACAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTACCAGCGGCATGTAGGTTGTGCCg                                                                                                       >  2:10190/1‑251 (MQ=255)
                                                                                                                                      gCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCg                                                                                                       <  1:181035/251‑1 (MQ=255)
                                                                                                                                        ggAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGtt                                                                                                     >  1:270644/1‑251 (MQ=255)
                                                                                                                                           agacTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGGGCAGATCCGCAAACACCAGTTCGCTTTGGGGATG‑TTCAGGCGTAAAACGAAGA‑GATTGTTTTGGGCGTAGATGTAGTTGGCAACGATGAAGCTGAAGGCAAACAGAATAACCACAAGGGTACC‑AAACTCAGCACCCCCGGAACCCATTAACACCCGCATCACCTTCTGGATAAACTGAATAACTTCCAGCAACATGTAAGATATGCCGTTAcc                                                                                                  >  1:303297/4‑251 (MQ=255)
                                                                                                                                            gacTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTAccc                                                                                                 <  1:392950/249‑1 (MQ=255)
                                                                                                                                                                                    gATCCGCAATCACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGAATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGGAGGTTGTGCCGTTACCCGCCAGTAATATCAGCAATGCGCTTGCCGTACAGATGAcc                                                          >  2:346452/1‑250 (MQ=255)
                                                                                                                                                                                      cccaaaaaCACCAGATCTCTTT‑GGGTTGGTTAGGGCG‑AAAAAAAAGAAGATGTTTTTCGGCGAAAATGTATTGGGCACCGATGGGGCAGAAGGAAAAAAAAAAAACCAGGAGGGAAAC‑AAACTCAGCCCCCAAGGACCCAATAAACCCCCGCATCCCCTTCTGGATAAGCTAAATCCCTCCCAGCGGCTTGTAGGTTGTGCCGTCCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAgg                                                       <  2:270644/246‑1 (MQ=255)
                                                                                                                                                                                              cccaagaccgttttgggtttg‑ttAAGGCGAAAAAAGAAGA‑GTTTGTTTGCGGGGAAAATGTAGTTGGAAACGAGGGAGCGGAAGGAAAACAAAAAAACCAGGAGGGAAAC‑AACCTCAGCCCCCCAGGACCCAATAACCCCCCGCATCGCTTTCTGGAAAAGCTGAATCCCTCCCGGCGGAATGTAGGTGGGGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGAt                                               <  1:223689/233‑1 (MQ=255)
                                                                                                                                                                                                      cGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGGGTCGATAAAAATGc                                       >  2:143587/1‑251 (MQ=255)
                                                                                                                                                                                                                    ttCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGAc                          >  1:233136/1‑251 (MQ=255)
                                                                                                                                                                                                                             aaaaaGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAAACATCTGGACAATCCCTTg                 >  2:295091/1‑251 (MQ=255)
                                                                                                                                                                                                                               aaaGAAGA‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTgcc               >  2:84996/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gaaga‑GATTGTTTTCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGCACCCATAAACACCCGCATCGCCTTCTGGATAAGCTGCATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCCTGGCGCtt                                                                         >  2:35622/1‑190 (MQ=255)
                                                                                                                                                                                                                                      gagttgtttttCGGCGAAAATTTAGTTGTAAACGAGGGAGCGGAAGGCAAACAAAAAAACC‑CCGGGGTAACCAACCCCACCCCCCCAGAACCCCATAACCCCCCGCATCCCCTTCGGAATAAGCTAAATCCCTCCCAGGGTCATGTGGGTTGTCCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCg         <  1:119273/244‑1 (MQ=255)
                                                                                                                                                                                                                                             ttttCGGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGCGCCGTTACCCGCAAGCAATATCAACATGGCGCTTGCCGTACAGATGAACAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCgg  >  1:311649/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
CGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTG‑TTCAGGCGTAAAAAGAAGA‑GATTGTTTTCGGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAAC‑AAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGG  >  NC_000913/6607‑7092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑