| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,628,678 | A→C | intergenic (+131/+177) | nadR → / ← ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,628,678 | 0 | A | C | 97.0% | 85.2 / NA | 33 | intergenic (+131/+177) | nadR/ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (14/18); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
GGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGG‑CTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCA > NC_000913/4628430‑4628910 | gggggAGATGCTGGAAGAGAACAAGATCGAATGCGGGCGGGTTGAAGAGGAGGATTATGACAGCCGGTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGa < 2:173277/248‑1 (MQ=255) gtggAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGAt < 2:442689/251‑1 (MQ=255) aaaTAACGAATTCGTGCGGTTTGAAGAGGACGAGTATGACAGCCGTTGCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTCTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCg < 1:250330/250‑1 (MQ=255) attcgtgCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGaaaaggcg > 1:272887/5‑246 (MQ=255) atgagtgCGGGTTGCAGAGGACGATTATGACAGACGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTAAAAGGCGCGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACATCTATGG‑AAGAGGATGTGGGGATTAAACACGGTTTGAAAAACCCTGCACGGTGGACCCGCTACGAAATTGCGGATAATTGGG‑CTGTTGTAgggcgacaacgggg > 2:230044/5‑238 (MQ=255) gggTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCAcgcc < 1:235277/251‑1 (MQ=255) ggTTGAAGAGGACGATTATGACAGCCGGTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCAcgccg > 1:178681/1‑251 (MQ=255) gAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGGGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATc < 2:117590/251‑1 (MQ=255) aaggggcggtttaggacatccGTTTCCTGCGCTGCTGGGAGCTGGGGCGGGAGATGATGGGGGAGCAGAGATAACCGCGTTGAAACGGCTCAAAGGCGAGTTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATcc < 1:253817/231‑1 (MQ=255) cgATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGAAAATAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTc > 1:67165/2‑251 (MQ=255) tatgACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGt < 1:322169/247‑1 (MQ=255) atgacagcagTTTCCTGCGCTGCGTGGAGCGGGTGCGGGAGATGATGGGGGAGCAGAAATAACCGCGATGAAACGGCTAAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGGGACCCGCTACAAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCTTTCACGCCGTATCCGGCATTCGGTGc < 1:377086/242‑1 (MQ=255) atgACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGACGTATCCGGCATTCGGTGc > 2:443985/3‑251 (MQ=255) gTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGATTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGACGGAAAAAGCGTTCACGCCGTATCCGGCATTCggatatcaatgccc > 2:235967/1‑239 (MQ=255) tgcgctgcgTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGG‑CTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATgg > 2:383986/1‑250 (MQ=255) cgTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCtt > 1:161356/1‑250 (MQ=255) gTGCGGGAGATGATGGGAGCGCAGAGCTAACAGAGATGAAAAGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGCAACCCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATAATAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCAGGCATTCGGTGCTCAATGCCTGATGCGACGATTACCGCGTCtt > 2:206899/1‑250 (MQ=255) gagaTGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATACGGCATTCGGTGCGCAATGCCTGATGAGACGCTTACCGCGTCTTATCAGGc > 2:373100/1‑251 (MQ=255) aTAACCGCGATGAAACGGCACAAAGGCGAGGT‑TCAAAAAAATTTTTTTGATTGGTGGAAACCCTCT‑TGGCAAGAGGCCGTCGAGATAAAACAAAGTTTTAAAAAACCTGCACGGGTGCCCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCGGGAAAAGGTGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTAtt < 1:93043/251‑1 (MQ=255) aaCCGCGATGAAACGGCTCAAAGGCGAGTTATAAAAT‑AATTTTTTTGATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTg < 1:383935/250‑1 (MQ=255) cTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttgt > 1:3273/1‑239 (MQ=255) gttGATGAGTTGAAATCCTCTCGGG‑AAGATGCTGTCAAGATTAAACAAGGTTTGACAAACCCTGCACGGGTGACCCGCTACGAAATTGCGGATCCTAAGG‑CTGTTGTAGGCCGGAAAAGTCGTTTACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:166798/249‑1 (MQ=255) tgtatgaGTTGAAACCCTATCTGG‑AAGAGGTTGTCGAGATTAAACAAGTTTTGAAAAACCCTGCACGGGTGACCCGCCACGAAAGTGCGGAT‑ATAAGGGCTTTTGTAGGCCGGAAAAGGCGTTCACTCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:196238/247‑1 (MQ=255) gaGTTGAAAACTTCTCTGG‑AAGAGGCGGTCGATAGTAAACAAGGTTAGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGAAACTAAGT‑CTGTGGTAGGCGGGAAAAGTCGTCCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGc < 1:294763/221‑1 (MQ=255) gTTGAAACCCTCTCTGG‑AAGAGGCGGTGAAGATTAACCAAGGTTTAAAAAAACCTGCCCGGGTGCCCCGCTACGAAATTCCGGATCTTAGGG‑CTGTTTTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTTTCAGGCCTGGAGATTCACAACGTATTGAAATTGCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAttt < 1:392895/251‑1 (MQ=255) agGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGATCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATAc > 2:407893/1‑251 (MQ=255) gtttacGAGATTAAAAAAGGTGTGAAAAAACCTGACCGGGGCCCCCGGTACGAAATTGCGGATACAAAGG‑CTGGGGTAGGCGGGAAAAGGCGGTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGGGGCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTCCACGCCTCATCCGGCATTTTACGCATTACTTCGCAATACGc < 1:175570/246‑1 (MQ=255) gagaTTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTAc > 1:347729/1‑251 (MQ=255) aaaCCCGCACGGGTGACCCCCTAGGAAATTGCGGATCCTAAGG‑CTTTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAgt < 1:193250/251‑2 (MQ=255) tGACCCGCTACGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCCATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGcccc > 1:332707/1‑250 (MQ=255) gtaCCCGCTACGAAATGGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCa < 1:87030/249‑1 (MQ=255) ctaCGAAATTGCGGATACTAAGG‑CTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttg > 1:445579/3‑135 (MQ=255) ctaCGAAATTGCGGATACTAAGG‑CTGTGGCAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttg < 2:445579/143‑11 (MQ=255) | GGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGG‑AAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGG‑CTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCA > NC_000913/4628430‑4628910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |