Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,574	A→G	G462G (GGT→GGC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,574	0	A	G	97.8%	128.8 / ‑5.9	46	G462G (GGT→GGC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base G (22/22);  minor base T (0/1);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

CGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGT  >  NC_000913/6334‑6818                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     cGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCg                                                                                                                                                                                                                                            >  1:71278/1‑251 (MQ=255) cGTCAGTTTCCGCGCATCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCg                                                                                                                                                                                                                                            <  1:196885/251‑1 (MQ=255)    catgtgccGCGCTTAATGGAGCAACGGCTGGGAATTGTCTAACAGCGCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGAt                                                                                                                                                                                                                                         <  2:28122/245‑1 (MQ=255)                      gATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAac                                                                                                                                                                                                                        >  2:437238/1‑250 (MQ=255)                       aTCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAacac                                                                                                                                                                                                                      >  1:143890/1‑251 (MQ=255)                         cAACGGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGTAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACAcc                                                                                                                                                                                                                     <  2:344521/250‑1 (MQ=255)                          aaCTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCgg                                                                                                                                                                                                                   <  2:381009/251‑1 (MQ=255)                                     aTTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGa                                                                                                                                                                                                        <  1:409484/251‑1 (MQ=255)                                           tAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTAc                                                                                                                                                                                                  >  1:341198/1‑251 (MQ=255)                                              cAGCTCCGGCAGCGTATAGCGCGGTGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTCAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACTCCGGGCGCTCGCCGAGTTTACGCt                                                                                                                                                                                               <  2:102995/251‑1 (MQ=255)                                                   ccGGCAGCGTAGAGCGCGTGGTGGTCAACGGGCTTTGGTAAGCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGc                                                                                                                                                                                            <  2:48326/249‑1 (MQ=255)                                                          cGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGATGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCACACACCGGGCGAACGCCGAGTTTACGCTGGCGTAGATAAt                                                                                                                                                                                   >  2:127060/1‑251 (MQ=255)                                                             ataGCGCGTGGTGGTCAATGGGCTTTGGTAAGCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGAAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCGGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCAc                                                                                                                                                                                <  2:227587/251‑1 (MQ=255)                                                                    gtggtgGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAAGCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGGTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGAGCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAAt                                                                                                                                                                         <  2:467607/251‑1 (MQ=255)                                                                            cAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGAGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAAAATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATACTCACTGGCAATGGTATGa                                                                                                                                                                  >  2:349269/1‑250 (MQ=255)                                                                                  gCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAg                                                                                                                                                           <  1:308388/251‑1 (MQ=255)                                                                                         tAATCAAGCGTTTTCGCAGGTGAAATAAGAAGCAGAATAGCCAATCCTGGCATGAAATTCATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGGTGTGAGTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTTGCGTCCGATATCCGGATAACGCAACGTACCAAACACCGGGCGCACGCCGAATTTACGCTAGCTTAGATAATCACTGGCATTGGTATGAACCACAGGCgaga                                                                                                                                                     <  2:227567/250‑1 (MQ=255)                                                                                                gCGTTTTCGCAGGTGAAATAAGAATCAACATATCCAGGCCTTGCAGGAAATTTATGACGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCAGCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTaaa                                                                                                                                             <  2:167076/251‑1 (MQ=255)                                                                                                 cGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCAACCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTaaaa                                                                                                                                            <  2:457179/251‑1 (MQ=255)                                                                                                             tGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAAt                                                                                                                                >  1:353719/1‑251 (MQ=255)                                                                                                                   aaGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGGCACATTGGTa                                                                                                                           >  2:181035/1‑250 (MQ=255)                                                                                                                        tCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGCGTTGATCGATATTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGACAGCTGGCGGCCGCTATCCGGATAACGAAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGACGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGGAAAATGGCGGTCAAATTgggtaaaagc                                                                                                                     >  2:256396/1‑243 (MQ=255)                                                                                                                               atCCATTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAgg                                                                                                              <  2:216321/251‑1 (MQ=255)                                                                                                                                           cAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAACGCTGGCGGCCGCTATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGCTAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCCGGCCGTTATTatat                                                                                                  >  2:207097/1‑251 (MQ=255)                                                                                                                                             ggAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCt                                                                                                <  1:437238/251‑1 (MQ=255)                                                                                                                                                aaTTTATGCCGACTTTATCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGcc                                                                                             <  1:17798/251‑1 (MQ=255)                                                                                                                                                aaTTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCATTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGcc                                                                                             <  1:218812/251‑1 (MQ=255)                                                                                                                                                              ttAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCa                                                                                <  1:365076/250‑1 (MQ=255)                                                                                                                                                                      aaaagagaatgagTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTGAAATTGGTAATAGCCATGCAGGCCATTATAATATCTGCCAGCTTCCACAACAGCGGaaaa                                                                        >  1:352611/5‑248 (MQ=255)                                                                                                                                                                      aaaagagaatgagTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAACCATGCCGGCCATTATGATATTTTCCAGTTGCCCCATCAGCGGaaaac                                                                       >  2:47182/5‑248 (MQ=255)                                                                                                                                                                                     gATCGATAGTTGTGATTACTCCTGCGAAACATCATCCAACGCGTCAGGAGAAAGCTGGCGGCCGATATCCGGATAAAGCAACGGATCAAAAACAGGGCGCACGCCGAGTTTACGCTGGAGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGGCAAATTGGGAATAGCCCAGCAGGAAATTATGATAACTACCAGTTGCAACATCAGCGGGAGACTTAGCAAGGcgccgc                                                        >  1:333607/1‑251 (MQ=255)                                                                                                                                                                                         gctAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATTGTATCTGCCAGTTTCCACATCAGCGGAAAGATTAGAAAGGTgccgaccgt                                                    >  2:392950/3‑246 (MQ=255)                                                                                                                                                                                             gttgtaagtACTCCCGCGAAACATCACCCCACGGTTCCGGAGAAGGCGGGCGGCCGTGAACCGGATAACGGAACGGAACAAACACCGTGCGAACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATAGTAGGAACCACAGGCGAGAGCAGTAAAATGGCTGTCAAACTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTGGCCACATCAGCGGAAGACTTGGCAAGGGGCCGCTGATGAcc                                                <  2:241193/243‑1 (MQ=255)                                                                                                                                                                                               tgtgATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATAGCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAACTGGCGGAAATATTGGTAATAGCCAGGCAGGCCATTAGGATATCTGCCAGTTGCCACATCAGCGGAAGACATAGCAATGTGCCGCCGATGGCCGt                                              >  1:156546/1‑251 (MQ=255)                                                                                                                                                                                                      aCTCTTGCGAAACATCATCCCCCGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGTAAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCTAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAg                                       <  2:323322/251‑1 (MQ=255)                                                                                                                                                                                                      aCTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCATTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGa                                                                                                     <  2:30757/189‑1 (MQ=255)                                                                                                                                                                                                      aCTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGGCATTATGa                                                                                                     >  1:30757/1‑189 (MQ=255)                                                                                                                                                                                                       cTCCTGCGACACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGCTCTCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTACCATTGGTAATAGCCGTGCCGGCCACTATGATATCTGCCAGATGCCCCATCAGCGGAAGGCTTAGCAAGGTGCCGCCGAttgacgatgcgaagg                                      >  2:221274/1‑237 (MQ=255)                                                                                                                                                                                                           cgCGAAACATCATCCCACGCTTCCAGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCTTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATAGCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCa                                  <  1:84389/250‑1 (MQ=255)                                                                                                                                                                                                              gAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGGAATAGCCACGCCGGCCATTATACTATCTGCCAGTTTCCACAGCAGCGGAAGCCTTCGCAAAGTGCCGCCGGAGACCGGTGCGacggggcaacg                               >  2:459145/1‑241 (MQ=255)                                                                                                                                                                                                               aaaCATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGc                                   >  1:78205/1‑246 (MQ=255)                                                                                                                                                                                                                           ccgggtcctggaaaagtgggcggcCGATATCCGGATAACGCAACGGATCAAACCCCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGAAATGGTATGAACCACAGGTGAGAGCAGTAAAATGGCGGGCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCCAACCCCAg                  <  2:383187/233‑1 (MQ=255)                                                                                                                                                                                                                           aCGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATCACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCCTTATAATATCTGCCCGTTGCCACCTAAGCGGAAAACTTAGCCAGGTGCCTCCGATGACCGTTTGGAAGGGGCAGCACCCCAAACACCa                   >  2:245146/1‑250 (MQ=255)                                                                                                                                                                                                                              cgTCCGGAGAAAGCTGGCTGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGtt                                                                                         <  2:288784/177‑1 (MQ=255)                                                                                                                                                                                                                              cgTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGAAGGCCATTATGATATCTGCCAGtt                                                                                         >  1:288784/1‑177 (MQ=255)                                                                                                                                                                                                                                   ggAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGGAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCCGATCGCttt          >  1:134692/1‑251 (MQ=255)                                                                                                                                                                                                                                           cTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCCTGCAGGCCATTATGATATCTTCCAGTTGCCACATCAGCGGAAAACTTAGCAAGGTGCCGCCGCTGGCCGTTGCGAAGGTGCAGATCCCCAAACACCAAAACCCATTAGGgttgt  >  2:481257/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGT  >  NC_000913/6334‑6818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑