Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,244	C→T	T310T (ACC→ACT)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,244	0	C	T	100.0%	162.1 / NA	52	T310T (ACC→ACT)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/30);  total (22/30)

ACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGT  >  NC_000913/4628011‑4628471                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    aCCGTGGCGATCCTTGGTGGCGTGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATTCCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTGTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACAGTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATGGACACTGATTTTGTcactaccca                                                                                                                                                                                                                    <  2:279857/251‑7 (MQ=255)  ccGTGGCGATCCTTGGTTGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAATCTTGCCAATATATTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTcactacccag                                                                                                                                                                                                                   <  1:14540/251‑8 (MQ=255)      ggCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCTCTACCCAGGCGt                                                                                                                                                                                                               <  1:116136/251‑1 (MQ=255)          aTCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTTAAACTGATTTTTTCACTACCCAGGCGTTCTg                                                                                                                                                                                                           >  1:243321/1‑251 (MQ=255)                 gtggcgAGTCGAGCGGTAAATCCACCCTGTTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAg                                                                                                                                                                                                    <  1:122377/249‑1 (MQ=255)                       aGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTTTTGACACTGATTTTGTCACTACCCCGGCGTTCTGCAAAAAGTACGa                                                                                                                                                                                               >  1:314166/1‑250 (MQ=255)                          cGAGCGGTAAATCCACCCTGGGAAACAAACTTGCCAATATCTTCAATACCTCCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAggg                                                                                                                                                                                           <  1:45040/251‑1 (MQ=255)                                gTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgtg                                                                                                                                                                                       >  1:187666/1‑247 (MQ=255)                                                                      aatacccccaGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGAGTTTGTCACTTCCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGttt                                                                                                                                               <  1:208931/244‑1 (MQ=255)                                                                       ataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTc                                                                                                                                              <  2:375063/249‑1 (MQ=255)                                                                         accaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCg                                                                                                                                             <  2:343130/250‑1 (MQ=255)                                                                                   cgggggAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAAGCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGa                                                                                                                                    <  2:58264/246‑1 (MQ=255)                                                                                   cGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCCCAATACATTGATTTTGCAGTGAAATATGCCCATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTTCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGa                                                                                                                                    >  1:58264/1‑249 (MQ=255)                                                                                   cGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATc                                                                                                                                  <  1:73849/251‑1 (MQ=255)                                                                                      gggAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCct                                                                                                                                <  1:66016/250‑1 (MQ=255)                                                                                                     aTTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACg                                                                                                                <  1:322976/251‑1 (MQ=255)                                                                                                          gTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATCCCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgt                                                                                                            <  1:108040/250‑1 (MQ=255)                                                                                                           tCTTTTCACACCTCGGCGGTGATGAGATCGCATTTCAGTATTTCGATTACGAAAAAATCGCGCTGGGCCACGCACAATACCTTTATTTTGCAGTGAAATATGCCAATAAAGGGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTATGCAAAAAAGACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGGATACCGGTTCGGTCTGGTGGTCCTGCTGGGGAAAAACACGCCgtgg                                                                                                          >  2:153365/1‑251 (MQ=255)                                                                                                                 cacaCCACGGCGGTGATGAGTTCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTCCCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGAATGAGGAATTCCGTGTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCg                                                                                                    <  1:46028/251‑1 (MQ=255)                                                                                                                   cacCGCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGa                                                                                                  <  1:121861/251‑1 (MQ=255)                                                                                                                    cccTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGAt                                                                                                 <  2:85353/250‑1 (MQ=255)                                                                                                                                     aTCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACCTTGATTTTGCAGTGAAATATGCCAATCAAGTGGCGTTTATTGACACTGATTTTGTCACTAACCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATAACGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCgg                                                                                >  1:227141/1‑251 (MQ=255)                                                                                                                                       cGCATTGCAGTATTCCGATTACGATCAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGc                                                                               >  1:269327/1‑250 (MQ=255)                                                                                                                                              cagtactccgataacgctaacctCGCGCTGGGCCACTCACAATACATTTATTTTGCAGAGAAATATGCCAATAAGGAGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGCAGGGCGTGAGCATCCTTTCGCACAGGCGTTGATTGATGAATACAGTTTCGATCTGGTGATCCTGCTGGAGAACAAAACGCCTTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTccgg                                                                       >  2:298891/23‑248 (MQ=255)                                                                                                                                              cAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGCTGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCggg                                                                       >  2:322169/1‑250 (MQ=255)                                                                                                                                                     ccgattACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGAAACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCg                                                                 <  2:366605/245‑1 (MQ=255)                                                                                                                                                        attACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCaaa                                                             <  2:243321/249‑1 (MQ=255)                                                                                                                                                                   aTCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGaa                                                  <  1:124694/251‑1 (MQ=255)                                                                                                                                                                     cgcgCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGGGGATCGCAAAGAGTTCCAGAACt                                                >  2:12951/1‑251 (MQ=255)                                                                                                                                                                          tGGGCCACGCACAGTACATTGATTTTGGAGTGAAATATGCCATTAAAGAGGCGTTTATTAACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGCAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGGTGAATACCGTTTCGATCTGGTGATCCTGCTGGTGAACAACACGCCGTGGGTGGCGGTTGGTTTACGCAGCCTCGGCAGTTCGGTGGAACGCAAAGCGTTCCAGAACTTGCtg                                           >  2:63140/1‑251 (MQ=255)                                                                                                                                                                            ggCCACGCACAATGCATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGTCACTGATTTTGTCACTCCCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGTGGATGGTTTACGCAGCCTCGGCAGTTCGGGGGATCGCAAAGAGTTCCAGAACTTGCtggt                                         <  1:95368/251‑1 (MQ=255)                                                                                                                                                                            ggCCACGCACAATACATGGAGTTTGCAGTGAAATATGCCAATAAAGTGGCTTTTATTGACACTGATTTTGTCACTACCCAGTCGTTCTGCAAAAAGTACGAAGGGCTGGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCTGCCTCGGCAGTTCTGTGGATCGCAAAGAGGTCCAGAACTTGCtggt                                         <  2:189985/251‑1 (MQ=255)                                                                                                                                                                                ccgcacAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGgag                                     <  1:480659/246‑1 (MQ=255)                                                                                                                                                                                  gcacAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCtggtgg                                       >  2:422154/4‑247 (MQ=255)                                                                                                                                                                                  gcacAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACAATGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCACGGCCGTTCGGTGGGACTCAACGAGTTACAGAAAGTGCtggtgg                                       >  2:253817/4‑247 (MQ=255)                                                                                                                                                                                   cacAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTGTATTGACACTGATTTTGTCACGACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGGTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATg                                  <  2:269327/249‑1 (MQ=255)                                                                                                                                                                                     cAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCt                                >  1:205444/1‑251 (MQ=255)                                                                                                                                                                                      aaTACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCtg                               >  1:403702/1‑250 (MQ=255)                                                                                                                                                                                         aCATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCTTTCTGCAAAAAGTACGAAGTGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGaa                            <  1:339407/251‑1 (MQ=255)                                                                                                                                                                                             tGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCGAAGAGTTCCAGATCTTGCTGGTGGAGATGCtggacg                           >  2:196238/1‑243 (MQ=255)                                                                                                                                                                                             tGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTGCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAagag                         <  2:128399/250‑1 (MQ=255)                                                                                                                                                                                                  ttGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACaa                    <  1:427413/250‑1 (MQ=255)                                                                                                                                                                                                   tgcagTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAat                   >  1:455043/5‑250 (MQ=255)                                                                                                                                                                                                      agTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCg               <  1:309822/250‑1 (MQ=255)                                                                                                                                                                                                          aaaTATGCCAATAAAGTTGCGTTTAGTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAAtt           <  2:314166/251‑1 (MQ=255)                                                                                                                                                                                                          aaaTATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAAtt           >  1:180784/1‑251 (MQ=255)                                                                                                                                                                                                          aaaTATGCCAATAAAGGGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGATCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGGGGCGGATGGTTTCCGCAGCCTCGGCAGTTCGGGGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCtgggaggaaaaaatatcgactt           >  2:177602/1‑230 (MQ=255)                                                                                                                                                                                                            atatGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAg                                                                             >  2:257734/1‑183 (MQ=255)                                                                                                                                                                                                            atatGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAg                                                                             <  1:257734/183‑1 (MQ=255)                                                                                                                                                                                                             tatGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTc                                                                                                                                                                            >  2:326963/1‑87 (MQ=255)                                                                                                                                                                                                              atGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTcgtg       <  1:422154/251‑1 (MQ=255)                                                                                                                                                                                                                   aaTAAAGGGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGAACGCAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAAAGAACAATATCGAATTCGTGCgggg  >  1:71995/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    ACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGT  >  NC_000913/4628011‑4628471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑