Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,349	C→T	E37E (GAG→GAA)	yaaA ←	DNA binding and peroxide stress response protein YaaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,349	0	C	T	97.1%	99.4 / ‑6.3	34	E37E (GAG→GAA)	yaaA	DNA binding and peroxide stress response protein YaaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/14);  minor base A (0/1);  total (19/15)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGAT  >  NC_000913/6104‑6587                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               cgcaGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATg                                                                                                                                                                                                                                           >  1:216321/4‑251 (MQ=255)     gTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGACTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGGTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCCGTTTCCGCGCTTCATGGATc                                                                                                                                                                                                                                       >  1:175522/1‑251 (MQ=255)              aCAACCCGTAAAGCATCCGCAATTGCTGTGTGGCACAATCGAATTCTTCTTCGCTTAAGTTTTCGCCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGACTCGCCGGGCGGGCAT‑TCTCCGGCGTAAAATCGGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAActgctg                                                                                                                                                                                                                               <  1:321392/250‑1 (MQ=255)                   ccGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAAtt                                                                                                                                                                                                                         >  1:446879/1‑251 (MQ=255)                     ggAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGt                                                                                                                                                                                                                       >  1:323322/1‑251 (MQ=255)                       aaaGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCt                                                                                                                                                                                                                     >  1:119746/1‑251 (MQ=255)                               cGCAAATGCTGTGGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCAAGAATCGCCGGGGGGGTAT‑TCTCCGTCGTGAAATCTGTCTCCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCGACTGCTGGGAATTGTCTAACAGCTc                                                                                                                                                                                                             <  1:462138/251‑1 (MQ=255)                                 cAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGCTGCGCATCCGCGTGCTAATCTGCGGGGGCGTCGGTTTCCGCGCTTCATGGGTTAACTGATGGGCATTGTCTGACAGCTCCg                                                                                                                                                                                                           >  2:84389/1‑251 (MQ=255)                                                                  gCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCACGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGGTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCttt                                                                                                                                                                          >  2:103536/1‑251 (MQ=255)                                                                   cTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTACCAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTg                                                                                                                                                                         >  2:17798/1‑251 (MQ=255)                                                                      agggttccgcccTGAAAGCGGGTGTAGACATCACCTTAAACCGCCAGAACCGCCGGGCGGGCAT‑TCCCCGGCGGGAAATCGGGCTGCCAGTCATGAAAGCGAGGGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTa                                                                                                                                                                      <  2:93578/241‑1 (MQ=255)                                                                              ggccggaaagcctgtggaaaaaTCACCTTTAAACGCCAGAATCGCCTGGGGGGCATCTC‑CCGGGGTGAAAGGGGGCTGCCATGAATGAAAGCGAGCGGCGGTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAAGTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCg                                                                                                                                                              <  2:175522/230‑1 (MQ=255)                                                                                           gtgtaacaatccccTTTAAACGCCAGACCCGCCGGGGGGGTAT‑TTCCCGGGGGGAAAGCTGGCGGCCAGTAATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGa                                                                                                                                                 <  2:58918/239‑1 (MQ=255)                                                                                                     cactgtataaccctaGAATCGCTCGGCGGGCAT‑TCCCCGGCGTGAAATCTGCCTCCAAGTCTTGAAAGCGCGGGGCGTTGACCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATc                                                                                                                                       <  2:387777/237‑1 (MQ=255)                                                                                                        cTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGc                                                                                                                                    >  1:325209/1‑251 (MQ=255)                                                                                                           tAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAActgctg                                                                                                                                                                                                                               >  2:31334/1‑157 (MQ=255)                                                                                                           tAAACGCCAGAACCGCCGGGCGGGCAT‑TCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAActgctg                                                                                                                                                                                                                               <  1:31334/157‑1 (MQ=255)                                                                                                                                gggCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAAttt                                                                                                            >  1:48326/1‑251 (MQ=255)                                                                                                                                  ggcatcctccGGGGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAGTCAGCATATCCAGTCCTTGCAGGAAATTTa                                                                                                           <  1:326153/243‑1 (MQ=255)                                                                                                                                                                                  gTTGATACCCGCCGGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGGGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGACAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGAAAGTTGTGAtt                                                           >  2:376052/1‑251 (MQ=255)                                                                                                                                                                                       gcgccgtcAGTTTGTCGCGTATGCGCATCAGCGTGTTAAACGGCGGAGGTGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAAACAGCAAATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTAc                                                         <  2:266782/241‑1 (MQ=255)                                                                                                                                                                                       cccccGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTcc                                                      <  2:376440/249‑1 (MQ=255)                                                                                                                                                                                                  ttGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATAGACTGCTGGGGATTGTCTAACAGCTCAGGCAGCGTATCGCGCGTGGTGGTCAACGGGCTTTGGTAATCACGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCAAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAAcatc                                           >  2:172576/1‑251 (MQ=255)                                                                                                                                                                                                         tGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGCTAGTTGTGATTACTCCTGCGAAACATCATCCCAc                                    >  2:365076/1‑251 (MQ=255)                                                                                                                                                                                                          gATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCa                                     <  1:103536/249‑1 (MQ=255)                                                                                                                                                                                                          gATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCAcg                                   <  1:376052/251‑1 (MQ=255)                                                                                                                                                                                                                cATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCg                             >  2:103847/1‑251 (MQ=255)                                                                                                                                                                                                                 aTCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGTTTACTCCTGCGAAACATCATCCCACGCGTCCgg                            >  1:135348/1‑251 (MQ=255)                                                                                                                                                                                                                    agcattcTAATCCGCGGAGGCGTCAGTTTCCGCGCTGCAATGATTAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCTTGGGTGTCAACGGTCTTTGTTAATCAAGCGTTTTCGCATTTGAAATAAGAATCAGCATAGCCAGTCCTGGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGGGGTGATCGATAGTTGTGAGTACTCCTGCGAAACATCATCCCACGCCCCCGgaga                         <  2:300866/245‑1 (MQ=255)                                                                                                                                                                                                                        tGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGc                     >  1:344521/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAAAGGGCTTTGGTAATCAAGCGTTTTAGCAGGTGAAATAAGAATCAGCATATCAAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCggc              >  1:452265/1‑247 (MQ=255)                                                                                                                                                                                                                                        cGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCg     >  1:71278/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGTCAGTTTCCGCGCATCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCg     <  1:196885/251‑1 (MQ=255)                                                                                                                                                                                                                                           catgtgccGCGCTTAATGGAGCAACGGCTGGGAATTGTCTAACAGCGCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGAt  <  2:28122/245‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               CGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGAT  >  NC_000913/6104‑6587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑