Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,069	C→T	R386R (CGC→CGT)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,069	0	C	T	93.6%	136.5 / NA	48	R386R (CGC→CGT)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/21);  minor base G (1/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATG  >  NC_000913/4626831‑4627317                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                          cgcgTCCGTTGCTGGTGGAGATCCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCCCGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTCCTGATGGCGGTGGGTTCCAGCCTGCGGGACAGACCGCTg                                                                                                                                                                                                                                               >  2:267524/1‑251 (MQ=255)    gtccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGcc                                                                                                                                                                                                                                             >  1:63405/7‑250 (MQ=255)    gtccgttgctgGTGGAGATTCAGGCGCTGGGCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGcc                                                                                                                                                                                                                                             >  1:264871/7‑250 (MQ=255)          tgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAGCCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGCCGGCGTGAAGGTAACCGTAACCAGCGCCTGCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGa                                                                                                                                                                                                                                       <  1:445766/250‑1 (MQ=255)                   agaTTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGGCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCAACAGGATATGgtggtg                                                                                                                                                                                                                             >  1:182654/1‑251 (MQ=255)                                 gTCGATCACTCGATGATGGCTAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCgg                                                                                                                                                                                                               <  1:425647/251‑1 (MQ=255)                                 gTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCgg                                                                                                                                                                                                               >  1:48938/1‑251 (MQ=255)                                        acgcGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCa                                                                                                                                                                                                        <  1:404505/248‑1 (MQ=255)                                                    gtgaaacgcgccgcGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGc                                                                                                                                                                                            <  1:12972/243‑1 (MQ=255)                                                     gaatccgcgccgcGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGCCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGcc                                                                                                                                                                                           >  1:458906/8‑251 (MQ=255)                                                                 cgTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCg                                                                                                                                                                               >  1:204773/1‑251 (MQ=255)                                                                           gggCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATACGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGAACGCTGCCACAGGATCTGGTGGTGTTTGGCGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACg                                                                                                                                                                     >  2:415562/1‑251 (MQ=255)                                                                                        aCCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGCAGTCGGGCTGGCAGGGGAGATCCGCCCGGTTCCCAGCGGTCAGGAACGAATTTCAGAAgcg                                                                                                                                                        >  1:267204/1‑251 (MQ=255)                                                                                                      ctgctgGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCCCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGACCGGATTTCAGAAGCGGCGA‑AACACGGttt                                                                                                                                         >  2:10834/1‑251 (MQ=255)                                                                                                          gggcttgcgtGCACCGGCACGGGGGGCTGCAAATGGCCGTTCAGGATGGGTTTGGGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGc                                                                                                                                     <  2:457861/242‑1 (MQ=255)                                                                                                                   tGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGCTGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGACTTTCAGAAGCGGCGA‑AACAAGGGTTTCGCCGGGCGAtt                                                                                                                            >  2:34586/1‑251 (MQ=255)                                                                                                                         gTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCcg                                                                                                                      >  1:14909/1‑251 (MQ=255)                                                                                                                           cACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCcgg                                                                                                                     <  1:97234/250‑1 (MQ=255)                                                                                                                              ggtggtCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGACGGGCGATTGTTCCggcggc                                                                                                                 >  1:337058/1‑251 (MQ=255)                                                                                                                               gtggtCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCt                                                                                                                <  1:764/251‑1 (MQ=255)                                                                                                                               ggggtCGGCAAAGGGCCGATCAGGAGGGGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCt                                                                                                                <  2:458906/249‑1 (MQ=255)                                                                                                                                   tCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGGGATTGTTCCGGCGGCTAACg                                                                                                            >  2:384656/1‑251 (MQ=255)                                                                                                                                     tGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGGGGTGTTTGGTGAAGTCGGGCTGGCAGGGGCGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGGTTGTTCCGGGGGCTAACGt                                                                                                           >  2:364009/1‑250 (MQ=255)                                                                                                                                      gCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGtac                                                                                                         <  2:48938/251‑3 (MQ=255)                                                                                                                                            ggCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGaaa                                                                                                    <  1:212386/250‑1 (MQ=255)                                                                                                                                              ccGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGaaaaaa                                                                                                 <  2:91866/251‑1 (MQ=255)                                                                                                                                              ccGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGAGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGaaaac                                                                                                  >  1:2393/1‑249 (MQ=255)                                                                                                                                                  tCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGGGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATGGTTCCGGCGGCTAACGGACCGCAAAAAgcgc                                                                                             >  2:5398/1‑251 (MQ=255)                                                                                                                                                  tCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGAGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAACAgcgc                                                                                             >  2:133570/1‑251 (MQ=255)                                                                                                                                                   cAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGcc                                                                                            >  1:78024/1‑251 (MQ=255)                                                                                                                                                        gggtggtgtgagaggggactgcgtcgTGAAGGGATCCGAATCCAGCGCCGTCTTAGCATTACTGCTGGCGATGTGTGCCAGCATGCGTGACAGTGAGCGGCCAGAGGAGCTGGGGGTGTATGTATAAGTCGGGCTGGCTGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGTGA‑AACACGGGTTTCGGCGGGTGATTGTTCCGGTTGTTAACGTACCGAAAAAAGCGGCGGAAg                                                                                       <  2:460729/227‑1 (MQ=255)                                                                                                                                                         gtgtTTGTGAACTGGGTCGGGGGCGTGAAGGTAACCGAAACCAGCGCCGACTGAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAgg                                                                                      <  2:343875/251‑1 (MQ=255)                                                                                                                                                          tgtTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAggg                                                                                     >  1:135497/1‑251 (MQ=255)                                                                                                                                                                ggAACGTGGTCGGCGGCGGGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAg                                                                               <  2:446654/250‑1 (MQ=255)                                                                                                                                                                                ggTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGTTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaaa                                                               <  1:34586/250‑1 (MQ=255)                                                                                                                                                                                cgTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaa                                                                >  2:424901/1‑250 (MQ=255)                                                                                                                                                                                        taccttccccgagctcctccTTAGAGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTcc                                                       >  1:155416/20‑251 (MQ=255)                                                                                                                                                                                                        cGACCTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGCCCGCGGCCACAGGATCTGGTTGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCgtgt                                       <  1:258780/251‑1 (MQ=255)                                                                                                                                                                                                                 gTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGGGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGACAAC‑CGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGCAGGGATGCAGATTTTTGGCGGTAAAAACATCTCCGACGCCCTTTGCGTGTTCGCCGACt                              >  2:135070/1‑251 (MQ=255)                                                                                                                                                                                                                    aCTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTtat                           <  1:473478/251‑1 (MQ=255)                                                                                                                                                                                                                     cggctgGCGATGGGTTCAAGCCTGCGGGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTtata                          <  2:72453/249‑1 (MQ=255)                                                                                                                                                                                                                       gctgGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGGTTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAAt                        >  2:164096/1‑251 (MQ=255)                                                                                                                                                                                                                       gctgGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATaa                         <  1:293936/250‑1 (MQ=255)                                                                                                                                                                                                                          ggCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATgag                     <  1:280454/251‑1 (MQ=255)                                                                                                                                                                                                                          ggCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATg                       <  2:135497/249‑1 (MQ=255)                                                                                                                                                                                                                           gCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACTGGATCTGGTGGTGTTAGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCAGGTGCCCAGCGTTCAGGAACGAATTTCAGAAGCGGCGA‑AACACTGTTTTCGCCGGGCGGTGGTTCCGGAGGCTAACGTACTGACAAAAGCGCCGGAAGGGATGCAGATATTTGGCGTTAAAAAACTACCCTAAGCGCTGAACGTGTTCGTCGAGTTATAATgggg                    >  2:345889/1‑248 (MQ=255)                                                                                                                                                                                                                            cGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATgagga                   <  1:164096/251‑3 (MQ=255)                                                                                                                                                                                                                             gatggtgcccAGCCTGCTGGACCGACCGCGGCAACAGGATCGGGTGGTGTTTGGTGAAGGCGGGCTGGCAGGGGAGATACGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAAGgaggaa                  <  2:9517/243‑4 (MQ=255)                                                                                                                                                                                                                                             cgcgACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCACATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgagagatt  >  2:77465/1‑247 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                          CGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGA‑AACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATG  >  NC_000913/4626831‑4627317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑