Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,943	G→C	V344V (GTG→GTC)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,943	0	G	C	100.0%	141.3 / NA	42	V344V (GTG→GTC)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/18);  total (24/18)

GGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAG‑C‑TCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTT  >  NC_000913/4626700‑4627182                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ggTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCAcc                                                                                                                                                                                                                                           <  1:359403/251‑1 (MQ=255)      aTGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGCTTCAGGCGCTGGTCGATCACTCGATGATGGCGTATCCGCGCCGCGTGGCAGTGGGGCTGGAACACAACCGTATGGCAATCCTGCTGGCTGCCTTGCACCGtacc                                                                                                                                                                                                                                      >  2:413595/1‑248 (MQ=255)             gggCGTCGGCGCGATGAACGAACAGGGGATGCGTGAAGTCAGCAACCCTTCGGCAATTTTCGTAAGTCGCGGAGATGAAGTGACCGCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACggtggt                                                                                                                                                                                                                                <  2:51192/250‑1 (MQ=255)              ggCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCTCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTTCACCGTACCGGTGGTCt                                                                                                                                                                                                                              >  2:214503/1‑251 (MQ=255)               gggccTTCGCGATGACCGAACAGGGGCTTCGGGAAGTCAGCAACCCTGCGGCAATTTTCGTAAGTCGCGGATATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTg                                                                                                                                                                                                                             <  2:423422/247‑1 (MQ=255)                         gATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCg                                                                                                                                                                                                                   >  1:72453/1‑251 (MQ=255)                               cGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAgg                                                                                                                                                                                                             <  1:96014/251‑1 (MQ=255)                                 aaCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGAt                                                                                                                                                                                                           >  1:233169/1‑251 (MQ=255)                                 aaCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTAGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGGGGAGATTCAGGCACTGGTCGGTCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGCCTGGCAATCCTGCTGGCTGTCTTTCACCGGCAGGGTGGTCTGCAAATGGCCGATCAggct                                                                                                                                                                                                           >  1:9517/1‑249 (MQ=255)                                      gggCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGa                                                                                                                                                                                                                  >  1:213920/1‑239 (MQ=255)                                      gggCTGCGTGAAGTCAGCAACCCTGCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGa                                                                                                                                                                                                                  <  2:213920/239‑1 (MQ=255)                                           gCGTGAAGTCAGCATCCCTGCGGTAATTTTCTTAATGCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGGGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTtgt                                                                                                                                                                                                   <  2:347776/249‑1 (MQ=255)                                              ggAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGGTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACg                                                                                                                                                                                              <  2:413023/250‑1 (MQ=255)                                                  gTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGAGGAGATTCAGGAGCTGGTCGATCACTCGATGATGGCGAATCCGCGACGAGTGGCAGTGGGGCTGGAAAAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTATGCAAATGGCCGTTAAGGATGTGTTGTTGAACG‑TGGt                                                                                                                                                                                         >  1:334216/1‑251 (MQ=255)                                                     aGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTcgg                                                                                                                                                                                      >  1:92991/1‑251 (MQ=255)                                                               cGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGCTCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGa                                                                                                                                                                              >  1:49711/1‑249 (MQ=255)                                                                 gCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGCTGATGGCGACTCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACCGTGGTCTGCCAATGGCCGATCCGGCTGTGTTTATGAACGCTGG‑CGGCGGCGTGACGGt                                                                                                                                                                          >  2:17055/1‑251 (MQ=255)                                                                  cAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGt                                                                                                                                                                          >  1:341481/1‑250 (MQ=255)                                                                             aGTCGGGGAGATGAAGTGACGTCCGGCAGGC‑GC‑GTGATGGTGGTGTGGGAAGGAACGCGTTCGTTGCTGGTGGAGAGTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAg                                                                                                                                                              <  2:137365/251‑1 (MQ=255)                                                                                   ggagATGAAGTGACCTCCGGCAG‑CCTC‑GTGATGGGGGGGGGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAAGCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGa                                                                                                                                                        <  2:204339/248‑1 (MQ=255)                                                                                      gATGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACtt                                                                                                                                                     <  1:45882/251‑1 (MQ=255)                                                                                       aTGAAGTGACCTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTa                                                                                                                                                    <  1:389398/251‑1 (MQ=255)                                                                                           aGTGACCTCCGGCAG‑C‑GCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGt                                                                                                                                                <  1:396544/251‑1 (MQ=255)                                                                                                ccTCCGGCAG‑C‑TCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTActg                                                                                                                                           <  1:414251/251‑1 (MQ=255)                                                                                                               cgTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGCCTTAGCGTTACTGCTGGCGATGGttt                                                                                                                              >  1:326597/2‑251 (MQ=255)                                                                                                                           gtGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAAAGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGGTTCCAGCCTgcgtg                                                                                                                  >  2:381675/2‑249 (MQ=255)                                                                                                                                      cgcgTCCGTTGCTGGTGGAGATCCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCCCGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTCCTGATGGCGGTGGGTTCCAGCCTGCGGGACAGACCGCTg                                                                                                       >  2:267524/1‑251 (MQ=255)                                                                                                                                         gtccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCa                                                                                                                           <  1:332495/222‑1 (MQ=255)                                                                                                                                         gtccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGcc                                                                                                     >  1:63405/7‑250 (MQ=255)                                                                                                                                         gtccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑GGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCa                                                                                                                           >  2:332495/7‑228 (MQ=255)                                                                                                                                         gtccgttgctgGTGGAGATTCAGGCGCTGGGCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGcc                                                                                                     >  1:264871/7‑250 (MQ=255)                                                                                                                                               tgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAGCCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGCCGGCGTGAAGGTAACCGTAACCAGCGCCTGCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGa                                                                                               <  1:445766/250‑1 (MQ=255)                                                                                                                                                        agaTTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGGCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCAACAGGATATGgtggtg                                                                                     >  1:182654/1‑251 (MQ=255)                                                                                                                                                                      gTCGATCACTCGATGATGGCTAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCgg                                                                       <  1:425647/251‑1 (MQ=255)                                                                                                                                                                      gTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCgg                                                                       >  1:48938/1‑251 (MQ=255)                                                                                                                                                                             acgcGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCa                                                                <  1:404505/248‑1 (MQ=255)                                                                                                                                                                                         gtgaaacgcgccgcGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGc                                                    <  1:12972/243‑1 (MQ=255)                                                                                                                                                                                          gaatccgcgccgcGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGCCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGcc                                                   >  1:458906/8‑251 (MQ=255)                                                                                                                                                                                                      cgTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCg                                       >  1:204773/1‑251 (MQ=255)                                                                                                                                                                                                                gggCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATACGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGAACGCTGCCACAGGATCTGGTGGTGTTTGGCGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACg                             >  2:415562/1‑251 (MQ=255)                                                                                                                                                                                                                             aCCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGCAGTCGGGCTGGCAGGGGAGATCCGCCCGGTTCCCAGCGGTCAGGAACGAATTTCAGAAgcg                >  1:267204/1‑251 (MQ=255)                                                                                                                                                                                                                                           ctgctgGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCCCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGACCGGATTTCAGAAGCGGCGAAACACGGttt  >  2:10834/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAG‑C‑TCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACG‑TGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTT  >  NC_000913/4626700‑4627182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑