| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,626,673 | G→A | L254L (TTG→TTA) | radA → | DNA recombination protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,626,673 | 0 | G | A | 98.3% | 172.4 / NA | 61 | L254L (TTG→TTA) | radA | DNA recombination protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/37); total (23/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCG‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCT > NC_000913/4626430‑4626909 | gATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGcc < 1:380788/251‑1 (MQ=255) gACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAAcc < 2:305367/250‑1 (MQ=255) gTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGt < 1:298075/251‑1 (MQ=255) gTGATGCATATGGCGGATGTACAGACATCGCCTGGCAGCGGGGAGCAGGTGCGGGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACCCGTAACCAAAGATGGCCCGCTGGCGGGCCCT‑TAAGTGCTGGGACACTGGTA‑CGCATGTTCGGTGCCTTTGGAAGGGCGAT‑CAGCCTCCCGTTTTCCCAACTTACGCCGCCATAAAAACCCCttaggggaggg > 2:396544/1‑242 (MQ=255) aTGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGaa < 1:35723/251‑1 (MQ=255) gCATAGGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATg < 2:372209/251‑1 (MQ=255) atatGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACCCGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑GATCGACTGTTCGGGGCTATTGAA‑TGGCGTTGCCGACTCCCGGTTTCGCACTTTACGCAGCCATAAAAACCGCTTCAGGGCGGTGAATg > 1:457861/1‑249 (MQ=255) atGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGTCATGGTTGGAAACGTAACCAAAGATGGCTCGCTGGGGGGCCCT‑AAAGAGGGGGAGCACTG‑TTTCGACTGGTCGGGGCGTTTGGA‑TGGGGAGGCCGGCTCCCGTTTTTGCAATTTACGCCGCCCTAAAAACCCCTTCgggccgggggatgggat > 2:292824/1‑236 (MQ=255) cGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGGGCTGGAACCCTG‑GATCGACTGTTCGGTGCTTTTGGG‑GGGCGGTGCCGACCCCCGTTTTCGCACTTTACGCAGCCATAAAAACCggctcgggcgggggaatgggctgggg > 2:45882/1‑226 (MQ=255) accgTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTAACTGACTCGCTTCTCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGTTCGCTGGCGGGCCCT‑AAAGTTCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATGAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcg < 2:176099/248‑1 (MQ=255) cTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGa > 1:51192/1‑250 (MQ=255) gggggCGCTGGGGCGTGAAACGGTGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTTGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGc < 2:16368/248‑1 (MQ=255) tGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATAGTCATGGATGGACACGTAACCAAAGATGGGTCGCTGGCGGGCCCT‑ATAGTGCTGGACCACTG‑TATCGACTGGTCGGGGCTTTTGGA‑TGGCGATGCCTACTCCCCGTTTCTCACTTTTCGCAGCCATAAAAAACGCTTCGGCGCGGTGGATGAGACGTGCGACTTCGCCTAGGACCGAACAgggctg > 2:293936/1‑248 (MQ=255) gcAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTg > 1:137365/1‑251 (MQ=255) ggTGCATGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGATTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAg < 1:9771/251‑1 (MQ=255) cGTGAAACGGCGGCTTATCGTACGCGCTTCGCCAAATCGCGCGGTGTGTCTATTTTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTGCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCTGCCATAACAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAg < 1:38551/251‑1 (MQ=255) tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGACCACTG‑TATCGCCTGTTCGGTGCTTTTGGG‑GGGCGATGCCGACTCCCGTTTTCGCCCTTTACGCAGCCATAAAAACCGCTTCGGCGCGGGGGCTGAGCTGGGCGTCTTCGC‑GATGCCCGAACAGGGGCTGCGTggaggcaac > 2:359403/1‑242 (MQ=255) tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCa > 1:197324/1‑248 (MQ=255) tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCa < 2:197324/248‑1 (MQ=255) ggcggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGTAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCtt < 1:175006/251‑1 (MQ=255) gtggcttacctGACGCGCCTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTTGACACGTAACCAAAGATGGCGCGCTGGCGTGCCCT‑AAAGTGCTGGAACACTG‑GATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTTAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTc < 2:293833/241‑1 (MQ=255) gcggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTc < 1:200037/251‑1 (MQ=255) ggtttagctGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTGGCGGGCGGGCCCT‑AAAGTGCTGGAACACGG‑TATCGACTGTGCGGTGCTTTTTGA‑TGGCGATGCCGACTCCCGTTGTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTGCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCgg < 2:162350/243‑1 (MQ=255) tacgcgattCGCCAAAACTCGCGGTGGGGCGATTGTCATGGGTGGATACGGAACCAAAGATGGTCCGCTGG‑TGGGCCGTAAAGTGCTGGACCACTG‑TATCGACTGTACGTTGTTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGTCCGAACAGGGTCTGCGTGAATACAGCAATATTTCGGCAATTTTc < 2:394924/243‑1 (MQ=255) gacgcGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAAtt < 1:241998/243‑1 (MQ=255) gcgcgtCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGGTGGGGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCGTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTaa < 1:61136/246‑1 (MQ=255) cGCTTCGCAAAAACGCGGGGTGTGGCGATTGTCATGGTGGGGCGCGTACCCAAAGATGGTTCGCTGGCTGGCCCG‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACGCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAg < 2:114656/251‑1 (MQ=255) cTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGCCTGCTCGGTGCCTTTGGA‑TGGCGCTGCCGACTACCGTTTTCGCCATTTACGCACACATAAAAACCACTTCGGCGCGGGTAATGAGCAGGACTGCTTCGC‑GATAGCCCACCCGGGGCTGCGTGATGTAAGACACACTTAGGCAATTTTCTTTAGTc > 2:405223/1‑251 (MQ=255) tCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTc < 1:144475/249‑1 (MQ=255) ccAAAACGCGCGGTGTGGCGATTGTAATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCgcgga < 2:331747/251‑2 (MQ=255) aaaaCGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGC‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCCC‑GATGGCCGAACAGGGGCTCCGTGAAGTCAGCAACCCTTCGGACATTTTTTTAAGTCgcgggg > 2:389398/1‑248 (MQ=255) ggggggTGTGGGGATTGTCATGGTTGGACACGGAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAg < 2:9683/247‑1 (MQ=255) gggcgGTGTGGCGATTGTCAGGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCTCT‑AAAGTGCTGGAACACTG‑TATCGACGGTTCGGTGCTTTTGGA‑GGGCGATGCCGACTCCCGTTTGCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAg < 2:469532/249‑1 (MQ=255) cgcgGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAgg > 1:413023/1‑250 (MQ=255) cgcgGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGAGGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGGACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGt < 1:32672/251‑1 (MQ=255) ggTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGAc < 1:221702/251‑1 (MQ=255) aTTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGGGCTGGAACCCTG‑GATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCCGCCATAAAAAACGCTTCGGCGCGGTGAATGGGCTGGGCGTCTTCGC‑GAAGACCGAAAAGGGGCTGCGGGACGTCAGCAACCCTTCGGCAATTTTCTTAAGGCgcggagtgggagggccctccggccg > 2:414251/1‑230 (MQ=255) tGTCATGGTTGGACACGTAACCAACGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTGTCTTAATTCGCGGAGATGAAGTGACCTCCGGCAGc < 1:483537/250‑1 (MQ=255) tGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGa > 1:102891/1‑239 (MQ=255) tGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGa < 2:102891/239‑1 (MQ=255) ggttggacACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑CAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGGGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGGGATGAAGTGCCCTCCGGCAGCTCCGTg > 2:473478/8‑250 (MQ=255) ggttggacACGTAACCAAAGATGGCTCGCTGGCGGGCCCT‑AAAGTGCTAGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGCCTTCGC‑GATGACCGAACCGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCCTAAGTCGCGGAAGTGAAGTGACCTCCGGCAGCTccgtt > 2:404505/8‑246 (MQ=255) ggacACGTAACCAAAGATGGCTCGGTGGCGGGCCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTGAATTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGTtggt < 1:130828/248‑1 (MQ=255) aTGGCTCGCTGGCGGGCCCT‑AAAGTGCTGGAACAATG‑TATCGCCTGTTCGGAGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCCTAAAAACCGCTTCGACGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGGTGGAGGTGTGGGAGGGAAcg > 1:425064/1‑251 (MQ=255) ggctcggtggcgggcCCT‑AAAGGGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGATTCACGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGGCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAAcgcg < 2:302943/244‑1 (MQ=255) ggcCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCtggt > 1:91866/1‑251 (MQ=255) ggcCCT‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTGTTCGCACTTTACGCAGCCATAAAACCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCtggt < 1:120539/251‑1 (MQ=255) ccctaaaGTGCTGGAACCCTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGTCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGTTCCGTGATGGTGGTGTGTGAAGGAACGCGTccgtttctggtgg < 1:171858/247‑12 (MQ=255) aaGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGAtt > 1:343875/1‑251 (MQ=255) ggAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGgc < 1:404747/249‑1 (MQ=255) gAACACTG‑TATCGACTGGTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCt < 1:161784/251‑1 (MQ=255) aCTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACTGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCg < 1:39303/251‑1 (MQ=255) cGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGAtggtgg < 1:29904/189‑1 (MQ=255) cGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGAtggtgg > 2:29904/1‑189 (MQ=255) tGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAACAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCCGCTCCGTGCTGGTGGTGTGGGAAGGACCGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCCCTCGGTGCTGGCg > 2:212386/1‑251 (MQ=255) gA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGaatccgcg > 1:446654/1‑245 (MQ=255) gttgttGCCGACTCCCGTTTTGTCACGTTACGCGTCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATTACCGAACAGGGGCGGCGTGAAGTTAGCAACCCTTCGGCAATTTTCTTAAGGCGCGGAGATGAAGTTACCGCCGGCAGCTCCGTGATGGTGGTGTGAGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATggttaatccgcgcc < 2:392566/245‑13 (MQ=255) ccGACTCCCGTTTTCGCACTTTACGCAGCCATAAAACCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGCTCACTCGATGATGGCGAATCCGCGCCGCGTGGcc > 2:280454/1‑250 (MQ=255) ccGACCCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCGCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCa < 2:76635/251‑1 (MQ=255) gcctccctgtttCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGGGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGt < 2:406707/242‑1 (MQ=255) cGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCt < 1:9160/251‑1 (MQ=255) | GATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCG‑AAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCT > NC_000913/4626430‑4626909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |