Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,521	T→C	L204L (TTG→CTG)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,521	0	T	C	100.0%	165.7 / NA	55	L204L (TTG→CTG)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/29);  total (26/29)

CAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTC  >  NC_000913/4626275‑4626762                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cagATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGac                                                                                                                                                                                                                                                  <  1:229379/251‑1 (MQ=255)  agATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGacg                                                                                                                                                                                                                                                 >  1:331747/1‑250 (MQ=255)       aaaaCGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCtt                                                                                                                                                                                                                                            <  1:177822/251‑1 (MQ=255)                  tGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGCCGCGCCTTGCCAAAAcgc                                                                                                                                                                                                                                 >  2:357774/1‑251 (MQ=255)                      cccGGAGAAGAGGCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAACCCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCTCgg                                                                                                                                                                                                                             <  2:173097/250‑1 (MQ=255)                         ggCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCGGACGCGCTTCTCCAAAACGCGCGgtgt                                                                                                                                                                                                                          <  1:103090/251‑1 (MQ=255)                           cGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGCTGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCCGCGTGGCGCAGGTGCGGGAAACGGCGGCTTATCTGACGCGCCTCGCCCAAACGCGCGGGGTgg                                                                                                                                                                                                                        >  2:111345/1‑251 (MQ=255)                           cGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAATAATCGCCTGGCAGCGGTGCGCCGGTGCCTGAAACGGCGGCTTTTCTGACCCGCTTCGcccacacagccggtgtgg                                                                                                                                                                                                                        >  2:9771/1‑235 (MQ=255)                                agTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGAtt                                                                                                                                                                                                                   <  1:176943/251‑1 (MQ=255)                                     cTGCAACAGGTGGCGATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGGGCCGGTGCGTGAAACGGAGGCTTATCTGGCGCCCTTCGCCCAAACGCGCGGCGTgggggttttcat                                                                                                                                                                                                              >  2:120539/1‑241 (MQ=255)                                             ggTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAAGTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCAtggttggac                                                                                                                                                                                                      <  1:191822/251‑6 (MQ=255)                                                  cAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGGACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCCCTTCGCCAAAACGCGGGGGGTGGCGGTTGTCTTGGTTGGGCACGtt                                                                                                                                                                                                 >  2:404747/1‑250 (MQ=255)                                                    aTGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTATCATGGTTGGACACGTAAc                                                                                                                                                                                               <  1:321269/251‑1 (MQ=255)                                                       cgcgcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCa                                                                                                                                                                                             >  1:129521/1‑250 (MQ=255)                                                                cGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGCGCAGATCTGCCTGATTGCCGAAGACGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTAAAGTCATCGCCTGGCAGCGTGGCGCAGGTGAGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAAAGCGCGGTTGGGCGATTGTCATGGGTAGACACGTAACCCAAGAttgctc                                                                                                                                                                                   >  1:460729/1‑246 (MQ=255)                                                                            ccGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGGTGGACACGTAACCAAAGATGGCTCGCTGGCGGGccc                                                                                                                                                                       >  1:359096/1‑251 (MQ=255)                                                                                          cccgtccgatatggAAACCAGCATCGTGCAGAGCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGAGGGACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAac                                                                                                                                                         <  2:200376/239‑1 (MQ=255)                                                                                                   gtCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATc                                                                                                                                                <  1:111345/250‑1 (MQ=255)                                                                                                      ggAAACCAGCATCGCGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTTAAGGGCTGGAacagaacgggag                                                                                                                                               >  1:200376/1‑240 (MQ=255)                                                                                                      ggAAACCAGCATCGAGCACATCTGCCTGTGTGCAGAAGAAGAGCAACCGAAGCTGAGGGTAATTGACGCGACCCAGTGGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTTAAACTGCGGCTTATCTTACGCGCTTCCCCAAAACGCGCGGTGTGGCGAGTGTCATGGTTGGACTCGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTG‑TATCgcct                                                                                                                                            <  2:425107/251‑4 (MQ=255)                                                                                                              gCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTg                                                                                                                                    <  1:338391/251‑1 (MQ=255)                                                                                                                aTCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGGGATGCAAATGGCGGATGGACAGTCATAGCCTGGCAGCGTGGCGCAGGGGCGGGAAACGGAGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGAGGGGCGATTGTCATGGGTGGAAACGTAACCAAAGAGGGCTCGCTGGCGGGCCCTAAAGAGCTGGAAAACTG‑TATCGACAGTTCGGGGCt                                                                                                                                  >  1:176099/1‑251 (MQ=255)                                                                                                                        gATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑Ta                                                                                                                                                  <  2:235181/227‑1 (MQ=255)                                                                                                                        gATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑Ta                                                                                                                                                  >  1:235181/1‑227 (MQ=255)                                                                                                                             gCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGCCTGTTCGGTGCTTTTGGA‑TGGCg                                                                                                                      >  1:76635/1‑249 (MQ=255)                                                                                                                                agATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGcc                                                                                                                 <  1:40660/250‑1 (MQ=255)                                                                                                                                  aTTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TAACGCATGTTCGGTGCTTTTGGA‑TGGCGATGCCgg                                                                                                               >  1:347776/1‑250 (MQ=255)                                                                                                                                             agagCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTc                                                                                                    <  1:148646/251‑1 (MQ=255)                                                                                                                                              gagCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCg                                                                                                   <  1:447822/251‑1 (MQ=255)                                                                                                                                                ccaccgtaaGCTGATGGTAATTGACGCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTACCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCa                                                                                                 <  1:127369/244‑1 (MQ=255)                                                                                                                                                    ccGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGGTTTTGGA‑TGGCGATGCCGAATCCCGGTTTCGCActtt                                                                                             >  1:423422/1‑248 (MQ=255)                                                                                                                                                      gAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTTGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTGCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCActttac                                                                                           <  1:104364/251‑6 (MQ=255)                                                                                                                                                        aGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCCAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCCGGCGGGGCCCAAAATGCTGGAAAACTG‑TATCGCCTTTTTGGTGCTTTTTGG‑TGGCGATGCCGACTCCCCTTTTCGCActtttcgc                                                                                         >  2:144475/1‑244 (MQ=255)                                                                                                                                                            gATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGcc                                                                                     <  1:380788/251‑1 (MQ=255)                                                                                                                                                                 tAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGGTTGTCATGGTTGGCCACGTAACCAAAGATGGCTCGCGGGCGGGCCCTAAAGTGCTGGAACACTG‑TATAGACTGTTTGGGGGGTTTGGG‑TGGGGATGCCGACTCCCCtttttgccctttaccccccccaca                                                                                 >  2:9160/1‑230 (MQ=255)                                                                                                                                                                      gACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAAcc                                                                            <  2:305367/250‑1 (MQ=255)                                                                                                                                                                                  gTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGt                                                               <  1:298075/251‑1 (MQ=255)                                                                                                                                                                                  gTGATGCATATGGCGGATGTACAGACATCGCCTGGCAGCGGGGAGCAGGTGCGGGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACCCGTAACCAAAGATGGCCCGCTGGCGGGCCCTTAAGTGCTGGGACACTGGTA‑CGCATGTTCGGTGCCTTTGGAAGGGCGAT‑CAGCCTCCCGTTTTCCCAACTTACGCCGCCATAAAAACCCCttaggggaggg                                                               >  2:396544/1‑242 (MQ=255)                                                                                                                                                                                     aTGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGaa                                                            <  1:35723/251‑1 (MQ=255)                                                                                                                                                                                       gCATAGGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATg                                                          <  2:372209/251‑1 (MQ=255)                                                                                                                                                                                         atatGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACCCGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑GATCGACTGTTCGGGGCTATTGAA‑TGGCGTTGCCGACTCCCGGTTTCGCACTTTACGCAGCCATAAAAACCGCTTCAGGGCGGTGAATg                                                          >  1:457861/1‑249 (MQ=255)                                                                                                                                                                                           atGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGTCATGGTTGGAAACGTAACCAAAGATGGCTCGCTGGGGGGCCCTAAAGAGGGGGAGCACTG‑TTTCGACTGGTCGGGGCGTTTGGA‑TGGGGAGGCCGGCTCCCGTTTTTGCAATTTACGCCGCCCTAAAAACCCCTTCgggccgggggatgggat                                                      >  2:292824/1‑236 (MQ=255)                                                                                                                                                                                               cGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGGGCTGGAACCCTG‑GATCGACTGTTCGGTGCTTTTGGG‑GGGCGGTGCCGACCCCCGTTTTCGCACTTTACGCAGCCATAAAAACCggctcgggcgggggaatgggctgggg                                                  >  2:45882/1‑226 (MQ=255)                                                                                                                                                                                                      accgTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTAACTGACTCGCTTCTCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGTTCGCTGGCGGGCCCTAAAGTTCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATGAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcg                                           <  2:176099/248‑1 (MQ=255)                                                                                                                                                                                                                 cTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGa                                >  1:51192/1‑250 (MQ=255)                                                                                                                                                                                                                        gggggCGCTGGGGCGTGAAACGGTGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTTGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGc                        <  2:16368/248‑1 (MQ=255)                                                                                                                                                                                                                          tGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATAGTCATGGATGGACACGTAACCAAAGATGGGTCGCTGGCGGGCCCTATAGTGCTGGACCACTG‑TATCGACTGGTCGGGGCTTTTGGA‑TGGCGATGCCTACTCCCCGTTTCTCACTTTTCGCAGCCATAAAAAACGCTTCGGCGCGGTGGATGAGACGTGCGACTTCGCCTAGGACCGAACAgggctg                       >  2:293936/1‑248 (MQ=255)                                                                                                                                                                                                                              gcAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTg                  >  1:137365/1‑251 (MQ=255)                                                                                                                                                                                                                                 ggTGCATGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGATTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAg               <  1:9771/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGTGAAACGGCGGCTTATCGTACGCGCTTCGCCAAATCGCGCGGTGTGTCTATTTTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTGCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCTGCCATAACAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAg           <  1:38551/251‑1 (MQ=255)                                                                                                                                                                                                                                       tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGACCACTG‑TATCGCCTGTTCGGTGCTTTTGGG‑GGGCGATGCCGACTCCCGTTTTCGCCCTTTACGCAGCCATAAAAACCGCTTCGGCGCGGGGGCTGAGCTGGGCGTCTTCGC‑GATGCCCGAACAGGGGCTGCGTggaggcaac          >  2:359403/1‑242 (MQ=255)                                                                                                                                                                                                                                       tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCa            <  2:197324/248‑1 (MQ=255)                                                                                                                                                                                                                                       tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCa            >  1:197324/1‑248 (MQ=255)                                                                                                                                                                                                                                             ggcggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGTAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCtt   <  1:175006/251‑1 (MQ=255)                                                                                                                                                                                                                                              gcggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTc  <  1:200037/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTG‑TATCGACTGTTCGGTGCTTTTGGA‑TGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGC‑GATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTC  >  NC_000913/4626275‑4626762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑