Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,625,377	T→C	R161R (CGT→CGC)	serB →	phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,625,377	0	T	C	100.0%	193.2 / NA	61	R161R (CGT→CGC)	serB	phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/31);  total (30/31)

TCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATACCTGCGCGACAAGCTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCG  >  NC_000913/4625138‑4625615                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               tcactcacCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCt                                                                                                                                                                                                                                     >  1:390732/1‑251 (MQ=255)             cGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTg                                                                                                                                                                                                                          >  1:198040/1‑250 (MQ=255)                                  gAAGCGCAACTGGCTTTCGCCCCCCTGGGGAAAATCCCGCGCCGGGGCCCGCCGGGTTTGCTGGGGAGGGACGTGGATTCCGCCGCCATCCAAATTGAAGGTTTTGAGGAAATTGCCAAACTGGCCGGAAGGGGCGAGATGGGGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGTTTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGt                                                                                                                                                                                                    <  2:352433/251‑1 (MQ=255)                                      cgcACCTGGAGGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGt                                                                                                                                                                                                <  1:397743/251‑1 (MQ=255)                                            tGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAAt                                                                                                                                                                                          >  2:439629/1‑251 (MQ=255)                                               aTGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTg                                                                                                                                                                                       <  1:382108/251‑1 (MQ=255)                                                 gcccccccccgggggAAAATCCCTCCCCTGCGCCCGCCGGTTTTGCTGGTGATGGCAAGGGATTCCCCCGCCATCCAGATTGAATGTATGGATGAAATTGCCAACCGGGCCGGAACGGGCGAGATGGGGGCGGAGGTAACCGAACGGGCGATGCGCGGCGACCGCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGcc                                                                                                                                                                                     <  2:377296/240‑1 (MQ=255)                                                     ccccGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTg                                                                                                                                                                                 >  1:141371/1‑251 (MQ=255)                                                        cGCTGGGGAAAACCCCGCACCGGCGCACGCCGGGTTTGTTGGTGATGGACTGGGATTCCACCGCCATCCAGATTGAATGGATGGATGAAATGGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATg                                                                                                                                                                              <  2:343223/251‑1 (MQ=255)                                                            ggggAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCa                                                                                                                                                                           <  1:254499/250‑1 (MQ=255)                                                                 aaaTCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTa                                                                                                                                                                     <  1:162568/251‑1 (MQ=255)                                                                          aCCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTg                                                                                                                                                            >  1:133707/1‑251 (MQ=255)                                                                               cgcACGCTGGGTTTCCGGGTGATGGACATGGATGCCCCCGCCAGCCAGATGGAATGTATTGATGAATTGGCCAACCAGGCGGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTCCCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCt                                                                                                                                                       <  2:8361/251‑1 (MQ=255)                                                                                      cGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCt                                                                                                                                                                       <  1:27724/228‑1 (MQ=255)                                                                                      cGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCt                                                                                                                                                                       >  2:27724/1‑228 (MQ=255)                                                                                      cGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATGGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTg                                                                                                                                                <  1:94804/251‑1 (MQ=255)                                                                                       gggTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTgg                                                                                                                                               >  1:378423/1‑251 (MQ=255)                                                                                       gggTTTGCTGGAGATGGACATGGATTCCACCGCCATCCAGATTGAATTTCTTGCTGAAATTGACAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGGAAGGCGCTGACGCCAATATTCTGCAACACGGGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTgg                                                                                                                                               >  2:311292/1‑251 (MQ=255)                                                                                         gTTTGCTGGTGAGGGACAGGGCTTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGGGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGaa                                                                                                                                             <  2:167846/251‑1 (MQ=255)                                                                                                ggTGAGGGACATGGATTCCCCCGCCATCCAGATTAAATGGATTGATGAAATGGCCAAACTGGCCGGAAGGGGCGAGATGGGGGCGGAAGTACCCGAACGGGCGATGCGCGGCAACCTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgg                                                                                                                                      <  2:240607/251‑1 (MQ=255)                                                                                                   gATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgggc                                                                                                                                    <  1:250808/250‑1 (MQ=255)                                                                                                    aTGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgggctg                                                                                                                                  >  2:165128/1‑251 (MQ=255)                                                                                                       gacagggttccctccaccATCCAGATGGAATGTATTGATGAACTGGCCAACCTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGAGGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGaa                                                                                                                               <  2:380620/235‑1 (MQ=255)                                                                                                         cagggattCCACCGCCATCCAGATTGAATGTATTGAGGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTACCCGAACGGGCGATGCGCGGCGAACCCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCGTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAg                                                                                                                             <  2:59888/244‑1 (MQ=255)                                                                                                                     cGCCATCCAGATTGAATTGATTGATGAAATTGCAAAACTGGCGGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCt                                                                                                                 <  2:4363/251‑1 (MQ=255)                                                                                                                     cGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCt                                                                                                                 <  1:165128/251‑1 (MQ=255)                                                                                                                     cGCCATCCAGATTGAAGGTATTGATGAAATTGCCAACCTGTCCGGATCGGGCGAGATGGTGGCGGAGGTAACCGACCGGGCGATGCGCGGCGAACTCGATTTTCCCGCCAGACTGCGCAGCCGCGTGGCGACGCTGATAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCt                                                                                                                 <  1:269072/251‑1 (MQ=255)                                                                                                                      gCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGCGCCCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATGGCCTc                                                                                                                >  2:260794/1‑251 (MQ=255)                                                                                                                        cATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCcg                                                                                                              >  1:147994/1‑251 (MQ=255)                                                                                                                         aTCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCcgg                                                                                                             >  1:279409/1‑251 (MQ=255)                                                                                                                         aTCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCcgg                                                                                                             <  1:380378/251‑1 (MQ=255)                                                                                                                            cAGATTGAATGTATTGATGAAATGGCCAAACTGGCCGGAACGGGCGAGAGGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCcggcgg                                                                                                          <  1:96114/251‑1 (MQ=255)                                                                                                                              gATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCACTATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGATGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCt                                                                                                        >  2:29000/1‑251 (MQ=255)                                                                                                                                      gTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTActttc                                                                                                >  1:431819/1‑251 (MQ=255)                                                                                                                                       tATTGATGAAATTGCCAACCTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTActttc                                                                                                <  1:15049/250‑1 (MQ=255)                                                                                                                                        aTTGATGAAATTGCCAACCTGGCCGGACCGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAGGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTActttcgt                                                                                              >  2:205226/1‑249 (MQ=255)                                                                                                                                         ttGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTActttcttt                                                                                             >  1:372122/1‑251 (MQ=255)                                                                                                                                           gatgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGGGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGc                                                                                           >  1:170688/1‑251 (MQ=255)                                                                                                                                             ggaatttGCCAAACGGGCCGGAACGGGGGAGAGGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACGCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTg                                                                                         <  2:279409/246‑1 (MQ=255)                                                                                                                                               aaaTTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGa                                                                                        >  1:69291/1‑250 (MQ=255)                                                                                                                                                  ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAAttt                                                                                    >  2:260009/1‑249 (MQ=255)                                                                                                                                                   ggcaaaaCCGGCCGGACGGGGCGGGAGGGGGGCGGAGTTAAACGAACGGGCGAGGCGGGGAGACCACGTTTTTACCGCCAGCCGGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGGGCTCAAGCTTGAACCGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGaagatc                                                                                   <  2:268837/247‑5 (MQ=255)                                                                                                                                                     ccAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCCGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGCGCTGGAAAGTGGCTATTGCCTCCGGCGGCTTTACTTTCTTTGCTGactatctg                                                                                 >  1:156989/1‑244 (MQ=255)                                                                                                                                                        aaCTGGCGGGAACGGGCGAGTTGGGGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCGGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTgcgc                                                                              <  1:446502/251‑1 (MQ=255)                                                                                                                                                                     gggCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTCATTTCTTTGCTGGATATCTGCGCGCCAAGTTGCGcc                                                                 >  2:386321/1‑251 (MQ=255)                                                                                                                                                                                gggcgaaataaacggacgggggggggcgcGGCAAACTCGATTTTACCGCCAGCCTGCGCCGCCGCGTGGCGACGCTGAAAGGCGCTGACGCAAATATCCTGCAACGGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGCCCGCCGTg                                                      <  2:390732/226‑1 (MQ=255)                                                                                                                                                                                   cGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGt                                                    >  1:325151/1‑250 (MQ=255)                                                                                                                                                                                    ggAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGttg                                                  >  2:400614/1‑249 (MQ=255)                                                                                                                                                                                                  gggCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTgg                                      >  1:366724/1‑249 (MQ=255)                                                                                                                                                                                                    gCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATAGCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGAt                                  <  1:391503/251‑1 (MQ=255)                                                                                                                                                                                                            cggcgAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACg                          >  1:82008/1‑251 (MQ=255)                                                                                                                                                                                                               ggaactggtttttCCCGCCAGCCTGCGCAGCGGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTAAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACGTTCGTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCAGGGACGGTa                       <  2:247665/242‑1 (MQ=255)                                                                                                                                                                                                                  atcctaTTTTCCCGCCAGCCGGCGCAGCCGCGGGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAAt                    <  2:133707/246‑1 (MQ=255)                                                                                                                                                                                                                      gATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTAc                >  1:93001/1‑251 (MQ=255)                                                                                                                                                                                                                          ttcccGCCAGCCTGCGCAGCCGCGTGGCGCAGCGGAAAGGCGCTGACGCCAATATCCTGCAACAGTTGCTTGAAAACCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGTTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATTTCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCgg             <  2:468331/247‑1 (MQ=255)                                                                                                                                                                                                                          ttACCGCCAGCCTGAGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTACCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGACAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGATCTGGAGATCATGGACGGTAAATTTACCGGc            >  1:19903/1‑251 (MQ=255)                                                                                                                                                                                                                           tACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGc            >  2:251398/1‑250 (MQ=255)                                                                                                                                                                                                                             ccGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAAt         >  1:386981/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCCTGCGAGGCCGCGTGGCGATGCTAAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCTGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGATGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATc   <  1:189948/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCg  <  1:251398/251‑1 (MQ=255)                                                                                                                                                                                                                                     atGCGCAGCAGCGTGGCGACGCTGAAAGACGCAGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATCTGCGCGACAAGTTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAAtg        >  1:367517/2‑244 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               TCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATACCTGCGCGACAAGCTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATCG  >  NC_000913/4625138‑4625615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑