Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,625,242	T→C	D116D (GAT→GAC)	serB →	phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,625,242	0	T	C	96.2%	152.7 / NA	53	D116D (GAT→GAC)	serB	phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (25/26);  minor base A (1/1);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTG  >  NC_000913/4624994‑4625487                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      cACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTTATGGatat                                                                                                                                                                                                                                                     <  2:240153/251‑1 (MQ=255)       ggTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGattc                                                                                                                                                                                                                                               <  1:325873/251‑4 (MQ=255)           gTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCGGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCAcc                                                                                                                                                                                                                                           <  1:441244/251‑1 (MQ=255)                               ggTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTAt                                                                                                                                                                                                                       >  1:352433/1‑251 (MQ=255)                                gTCGTTGGCTTGATAAGCATCGTCTGACACAATACTATAGCATACTGGGTGCGGCTATGGTGATTGTTGCCGCTTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTCCACGCACCGCACGGGCTACGTGCCTGGCCCACGAAGCGCAACTGTGTGTCGCCCCGCTGGGGAAAAGCTCGCACCTACGCACGCCGTGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTAtt                                                                                                                                                                                                                      <  2:7914/251‑1 (MQ=255)                                     gggCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGAATGAATGTATtgatga                                                                                                                                                                                                                 <  1:374444/251‑1 (MQ=255)                                      ggCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGaa                                                                                                                                                                                                                <  2:3722/251‑1 (MQ=255)                                            ataaacagcgtgagaaccAATACAAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGAGCGTGGAAGATTATCAGGTGATTCGTATGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGAAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGATGGGGCTGGAAATGGATTCCACCGCAATGCAGATTGAATGGATTGAGGAAAtgtac                                                                                                                                                                                                          >  2:319566/17‑247 (MQ=255)                                                acagcGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGCTTCCACCGCCATCCAGATTGCATGTATTGATGAAATTGCCAAAc                                                                                                                                                                                                      >  2:397743/5‑251 (MQ=255)                                                acagcGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGGGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCCCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGACTGTATTGATGAAATTGCCAAAc                                                                                                                                                                                                      >  1:236390/5‑251 (MQ=255)                                                     gTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGcc                                                                                                                                                                                                 >  1:346208/1‑251 (MQ=255)                                                                tACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGGTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAAAGGGCgg                                                                                                                                                                                      >  2:446502/1‑250 (MQ=255)                                                                    aaaacaaaaggggTGAGGCGATGTTGATTGGTGCCGCCTGGTGCGTGGAAGATTATCAGGTTATTCGGCTGGCAGGTTCACTCACCGCCTGGGCTACGCGCCGGGCCCATTAAGCGCAACTGTATGGCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGACGTGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGAtg                                                                                                                                                                                  <  2:262884/241‑1 (MQ=255)                                                                       gCAAACTGGGTTCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTCCGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGAtggtg                                                                                                                                                                               <  2:290144/251‑1 (MQ=255)                                                                         aaaCTGAGTGCGGCGATGGTGAATGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCCCCTGCGCACGCCGGGGTTGCTGGTGCTGGACATGGATTCCACAGCCATCAAGCCTGCATGTATTGATGAAATTGCCCAACTGGCCGGAACGGGCGAGAtggtggg                                                                                                                                                                             >  2:344464/1‑250 (MQ=255)                                                                                      cgATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGCTTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGGGATGGTGGCGGAAGTAACCGa                                                                                                                                                                 >  2:254499/1‑250 (MQ=255)                                                                                       aaTGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCAGCTTGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGGTTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGGAGTAACCGaa                                                                                                                                                                >  2:269072/2‑250 (MQ=255)                                                                                          ggTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACggg                                                                                                                                                            >  1:4363/1‑251 (MQ=255)                                                                                             gATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCgg                                                                                                                                                         >  2:162568/1‑250 (MQ=255)                                                                                              aTTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGAt                                                                                                                                                        >  1:247665/1‑251 (MQ=255)                                                                                              aTTGTTGCCGCCTGGTGCGTGGAAGATTAGCAGGTGTCTCGTCTGGCAGGTTCACTCACCGCACGGTCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGTACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGAt                                                                                                                                                        <  1:433473/251‑1 (MQ=255)                                                                                                    gccgccgtgTGCATGGAAGATTATCAGGTGATTTGTCCGGCAGGTTCCCTCTCCGCCCGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGcgcg                                                                                                                                                   <  1:263503/242‑1 (MQ=255)                                                                                                           ggggCGTGGAAGATTATCAGGTGATGCGTCGGGCAGGTTCGATCACCGCACGGGCTACGCGCCTGGTCCATGAAGAGCAACTGGATGTCGTCCCGCGGGGGAAAAGCCCGCAACTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGAGGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTc                                                                                                                                           <  2:250018/248‑1 (MQ=255)                                                                                                           ggTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTgca                                                                                                                                                                                                                            >  2:109289/1‑168 (MQ=255)                                                                                                           ggTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGaa                                                                                                                                                                                                                            <  1:109289/170‑1 (MQ=255)                                                                                                                       aTTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGCGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGcc                                                                                                                               >  2:380378/1‑251 (MQ=255)                                                                                                                                  aTTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTCGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCCGCCTgcgc                                                                                                                      >  2:391503/1‑249 (MQ=255)                                                                                                                                                 tcactcacCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCt                                                                                                     >  1:390732/1‑251 (MQ=255)                                                                                                                                                 accctcacCGCCCGGGGCACGCGCCTGGCCCATGAACCGCAATTGGATCTTCCCCCGTTGGGGAAAACCCCGCCCCGGTCCACGCCGGGTCTGCTGGTGAGTGACATGGATTCCGCCGCCATCCAGATGGAATGGATTGAAGAAATGGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGATCGGGGGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAgacgcttgtcgccgc                                                                                                      <  2:221253/247‑15 (MQ=255)                                                                                                                                                            aCGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATtgatga                                                                                                                                                                                                                 >  1:128071/1‑132 (MQ=255)                                                                                                                                                            aCGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATtgatga                                                                                                                                                                                                                 <  2:128071/132‑1 (MQ=255)                                                                                                                                                             cGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTg                                                                                          >  1:198040/1‑250 (MQ=255)                                                                                                                                                                                  gAAGCGCAACTGGCTTTCGCCCCCCTGGGGAAAATCCCGCGCCGGGGCCCGCCGGGTTTGCTGGGGAGGGACGTGGATTCCGCCGCCATCCAAATTGAAGGTTTTGAGGAAATTGCCAAACTGGCCGGAAGGGGCGAGATGGGGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGTTTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGt                                                                    <  2:352433/251‑1 (MQ=255)                                                                                                                                                                                      cgcACCTGGAGGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGt                                                                <  1:397743/251‑1 (MQ=255)                                                                                                                                                                                            tGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAAt                                                          >  2:439629/1‑251 (MQ=255)                                                                                                                                                                                               aTGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTg                                                       <  1:382108/251‑1 (MQ=255)                                                                                                                                                                                                 gcccccccccgggggAAAATCCCTCCCCTGCGCCCGCCGGTTTTGCTGGTGATGGCAAGGGATTCCCCCGCCATCCAGATTGAATGTATGGATGAAATTGCCAACCGGGCCGGAACGGGCGAGATGGGGGCGGAGGTAACCGAACGGGCGATGCGCGGCGACCGCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGcc                                                     <  2:377296/240‑1 (MQ=255)                                                                                                                                                                                                     ccccGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTg                                                 >  1:141371/1‑251 (MQ=255)                                                                                                                                                                                                        cGCTGGGGAAAACCCCGCACCGGCGCACGCCGGGTTTGTTGGTGATGGACTGGGATTCCACCGCCATCCAGATTGAATGGATGGATGAAATGGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATg                                              <  2:343223/251‑1 (MQ=255)                                                                                                                                                                                                            ggggAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCa                                           <  1:254499/250‑1 (MQ=255)                                                                                                                                                                                                                 aaaTCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTa                                     <  1:162568/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTg                            >  1:133707/1‑251 (MQ=255)                                                                                                                                                                                                                            cTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGAtt                                                                                                                                       >  2:222144/1‑142 (MQ=255)                                                                                                                                                                                                                            cTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGAtt                                                                                                                                       <  1:222144/142‑1 (MQ=255)                                                                                                                                                                                                                               cgcACGCTGGGTTTCCGGGTGATGGACATGGATGCCCCCGCCAGCCAGATGGAATGTATTGATGAATTGGCCAACCAGGCGGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTCCCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCt                       <  2:8361/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCt                                       <  1:27724/228‑1 (MQ=255)                                                                                                                                                                                                                                      cGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCt                                       >  2:27724/1‑228 (MQ=255)                                                                                                                                                                                                                                      cGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATGGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTg                <  1:94804/251‑1 (MQ=255)                                                                                                                                                                                                                                       gggTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTgg               >  1:378423/1‑251 (MQ=255)                                                                                                                                                                                                                                       gggTTTGCTGGAGATGGACATGGATTCCACCGCCATCCAGATTGAATTTCTTGCTGAAATTGACAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGGAAGGCGCTGACGCCAATATTCTGCAACACGGGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTgg               >  2:311292/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTTTGCTGGTGAGGGACAGGGCTTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGGGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGaa             <  2:167846/251‑1 (MQ=255)                                                                                                                                                                                                                                                ggTGAGGGACATGGATTCCCCCGCCATCCAGATTAAATGGATTGATGAAATGGCCAAACTGGCCGGAAGGGGCGAGATGGGGGCGGAAGTACCCGAACGGGCGATGCGCGGCAACCTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgg      <  2:240607/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgggc    <  1:250808/250‑1 (MQ=255)                                                                                                                                                                                                                                                    aTGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgggctg  >  2:165128/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      CACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTG  >  NC_000913/4624994‑4625487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑