Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,277	G→A	L171L (CTC→CTT)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,277	0	G	A	96.5%	172.9 / ‑3.9	57	L171L (CTC→CTT)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (24/31);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACA‑CCGATTGCCAGTGAGAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTC  >  NC_000913/4624030‑4624510                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             cGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAagc                                                                                                                                                                                                                                           >  1:132392/1‑251 (MQ=255)     catctGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAgca‑gca                                                                                                                                                                                                                                      <  1:246360/247‑1 (MQ=255)                aaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGt                                                                                                                                                                                                                           <  1:422683/251‑1 (MQ=255)                  aTACACTCCCCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCGCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTaa                                                                                                                                                                                                                         <  2:421456/251‑1 (MQ=255)                            ccACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAg                                                                                                                                                                                                                 <  2:205130/249‑1 (MQ=255)                               ccGCCAGGTTAAACCACGGGGCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAgtggtg                                                                                                                                                                                                            <  2:383654/251‑1 (MQ=255)                                 gCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGtt                                                                                                                                                                                                          <  1:300162/251‑1 (MQ=255)                                    aGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGGAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAagc                                                                                                                                                                                                                                           <  2:138625/216‑1 (MQ=255)                                      gTTAAACCTCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTGTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCa                                                                                                                                                                                                    <  2:402575/251‑1 (MQ=255)                                      gTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCa                                                                                                                                                                                                    <  1:54817/251‑1 (MQ=255)                                      gTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAAAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGGGTTAT‑ccc                                                                                                                                                                                                    >  1:401757/1‑250 (MQ=255)                                         aaaCCACTGGTCGTAAGAGTCCGAGATGAGCATGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTATTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTTCG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCAcc                                                                                                                                                                                                  <  2:286856/250‑1 (MQ=255)                                         aaaCCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGACGGTTACCCTGTAGCAGCGTGCGGGGC‑AACACTACG‑CCGATTGGCAGTGGAAGCA‑GCCACATACGGCGTAAAATGTTAatgggggttatacccc                                                                                                                                                                                                  >  1:381792/1‑239 (MQ=255)                                             cACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTg                                                                                                                                                                                            <  1:253141/251‑1 (MQ=255)                                             cACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTg                                                                                                                                                                                            <  1:403658/251‑1 (MQ=255)                                                gggTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GCCATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAAAAGAAGGGCGATTACGGCCCGCGGGGACGGTTTCCCTGTCGCCGAGTGGGGGTCCGGCAC‑ACC‑ACGATTGTCAGTGAAAGCC‑ACAAACTCAGGGGTAAAATATTATTGGTTTTAT‑CCACCTTGTTggg                                                                                                                                                                                          >  2:227361/1‑251 (MQ=255)                                                  gTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCtt                                                                                                                                                                                       <  2:201684/251‑1 (MQ=255)                                                         gagTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGGAGGGCGATTGCTGCCCGCGGGGACGTTTACCCTGTAGCAGCGGGCGGGGC‑CGCACTACG‑CCGGTTGCCAGTGAAAACA‑GCAACCTCAGGCGTAAAATGTTAGTGGGGTTAT‑CCACCT‑GTTGGGCTTCGGTggg                                                                                                                                                                                >  1:117005/1‑250 (MQ=255)                                                                     aGCAGGCGTAAT‑TGTGACATAACGATTTCCTTTCTTGTAGTAATGGGTCTCTCTTTTTTCGCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCT‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGgt                                                                                                                                                                    <  1:314818/251‑1 (MQ=255)                                                                                                   cTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGCTTTCTGCCAGCGGGTACGTTTTCCCTGTTGCAGCGGGCGGGGC‑AGCACTACG‑CCGATTGCCAGTTAAAGCA‑GCCACATCAGGCGGCAAAAGTTAGTGGTGTTAT‑CCCCCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGGCGGGCGGCAGGCGCAGATAGcccggcg                                                                                                                                       >  2:99725/1‑246 (MQ=255)                                                                                                     ttCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCgg                                                                                                                                      <  1:336341/250‑1 (MQ=255)                                                                                                      tCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAACGGAAGGGCGGTTGCTGCCAGCGGGGACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGGGAAAGCA‑GCAACATCAGGCGCAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGGGGGCGTATGGGGGTCGGGGGGCAGGCGCAggtaagcgggcggtg                                                                                                                                    >  1:3854/1‑237 (MQ=255)                                                                                                             gtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTTACAGGGTGGGCGGTTTCTTCCCCCGGGGACCTTTTCCCTGTAGCAGCGTGCGGGTC‑CGCCATACG‑CCGGTTGCCCG‑GGGAACAAGCAACATCCGGCGGAAAAAGTTCATGGGGTTAT‑CCACCT‑GGTGGGGTTCGGTGGGGAGGGTATAGGTGGCGTGCGTCAGgcgccggttacccgacgggcccttttt                                                                                                                             >  2:159467/1‑228 (MQ=255)                                                                                                                  gggTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGAGTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGtttttcccgg                                                                                                                        <  1:428319/251‑6 (MQ=255)                                                                                                                             ttttCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTggttc                                                                                                              <  2:401757/250‑4 (MQ=255)                                                                                                                             ttttCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGGGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGGAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTTGGCTTCGGTGGCGGGCGTATGGGGGTCGGGCGTCAGGCGCAGATAAACGAGCGGTCCGTTTTTCCCGGCCATTGGTTCg                                                                                                             >  1:391370/1‑251 (MQ=255)                                                                                                                                 ctcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGACGTTTACCCTGTTGCAGCGTGCGGGTC‑AGCACTACG‑ACGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCCCCT‑GTTGGGCTTCGGTGGCGGGCGGATGGGGGTCGGGCGTCCGGCGCAGATAGCCGAGCGGTCCGTTTTTTCCGGCCATTGGTTCGAcc                                                                                                          >  1:433673/1‑249 (MQ=255)                                                                                                                                  tcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTTCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGGGCGGGTC‑AGCACTACG‑CCGCTTGCCCGTGAAAACA‑GCAACATCCGGCGTAAAATGTTAGTGGTGTTATTCCAC‑T‑GTTGGGCTTCGGTGGCGGGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGGCCGGTTTTCCCGGCAATTGGTTCGTCAAt                                                                                                        >  1:439434/1‑251 (MQ=255)                                                                                                                                      tttCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGGAGGGCCATTGCTGCCAGCGGGTAAGTTTACCCTGTAGCCACGGGCGGGGC‑CGCCCTACG‑CCCGTTGCCAGTGAAAGCC‑GCAACATCCGGCGGTAAATTTTAGTGGGGTTAT‑CCCCCT‑GTTGGGGTTCCGTGGCGCGCGGATTGGGGTCGAGCGGCAGGCGCAGGTAACCGCGCGGGCCGttttttccggcccatggtttgcacctctgt                                                                                                    >  2:480212/1‑226 (MQ=255)                                                                                                                                          ttcctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTTCCCTGTAGCCGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGGAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGGGCGTATGGGGGTCGAGCGTCAGGCGCAGATAGCCGGGCGGTCCGTTTTTTCCGGCCATTGGTTCGACAATCTTCTGGt                                                                                                >  1:355266/1‑251 (MQ=255)                                                                                                                                           tcctcttccGGCCCCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGt                                                                                                <  2:13241/250‑1 (MQ=255)                                                                                                                                           tcctcttccGGCCCCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGt                                                                                                <  2:434238/250‑1 (MQ=255)                                                                                                                                           tcctcttccGGCACCGGTTTGCTGGCGTTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCTAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATctgctg                                                                                                  <  1:440692/248‑1 (MQ=255)                                                                                                                                           tcctcctccGGCACCGGTTTGCTGGCGGGTAACAGGAAGGGCCATTGCTGCCAGCGGGTACGTTTACGCTGGAGCAGCGTGCGGGTC‑AGACCTACG‑CCGATTGCCAGTGAAAGCA‑GCAACAGCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTGTTCCCGGCAATTGGTTCGACAATCTGCTgg                                                                                                 <  2:166404/249‑1 (MQ=255)                                                                                                                                                 tccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAAt                                                                                          <  1:78316/250‑1 (MQ=255)                                                                                                                                                    ggCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGACGTTTTCCCTGTAGCAGCGGGCGGGGC‑AGCCCTACG‑CCGGTTGCCCGTGGAAGCA‑GCAACCTCAGGCGGAAAATGTTAGTGGGGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGGGCGTGTGGGGGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCAGCTTTTTCCGGCAATTGGGTCGCCAATCTGCTGGTTAAAATAgcc                                                                                      >  2:241672/1‑251 (MQ=255)                                                                                                                                                         cGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTTGCGAGCGTATGTGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAgccgcccg                                                                                 <  1:285257/251‑3 (MQ=255)                                                                                                                                                           gTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTAAG‑CCGATTGCCCGTGTAAGAA‑GCCACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTTGGGGGCGGGCGTATGGGTGTCGGGCGTCAGGCGCAGGTAGCCGAGAGGTCCGTTTTTCCCGGCAATTGGTTCGCCAAATTGCTGGTTAAACTAcccgcccgcc                                                                               >  1:437045/1‑247 (MQ=255)                                                                                                                                                                     ggTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATCGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATATCCGCCCGCTTTTTTACCGt                                                                     >  2:482843/1‑251 (MQ=255)                                                                                                                                                                                     gATTGCTGCCAGCGGGTACGTTTACCCTTTAGCAGCGTTCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGGGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTc                                                     <  2:355266/251‑1 (MQ=255)                                                                                                                                                                                     gATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTc                                                     >  1:165367/1‑251 (MQ=255)                                                                                                                                                                                     gATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTc                                                     <  1:109060/251‑1 (MQ=255)                                                                                                                                                                                      aTTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGtttttcccg                                                                                                                         >  1:202075/1‑178 (MQ=255)                                                                                                                                                                                      aTTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTcg                                                    >  1:261567/1‑251 (MQ=255)                                                                                                                                                                                      aTTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGGGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGtttttcccg                                                                                                                         <  2:202075/182‑5 (MQ=255)                                                                                                                                                                                          ctgcCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCGGGCGCAGATAGCCGATCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCgcgc                                                 >  1:424883/1‑250 (MQ=255)                                                                                                                                                                                                  gggTCCGTTTCCCCTGTAGCAGCGTGCGGGGC‑AGCACT‑CAGCCGATCGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGGCGAGCGTCAGGCGCAGATATCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGGCGCGCACTTCGACg                                        <  2:179621/251‑1 (MQ=255)                                                                                                                                                                                                           tACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGgc                                                                                                                                                      >  1:177587/1‑132 (MQ=255)                                                                                                                                                                                                           tACCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGgc                                                                                                                                                      <  2:177587/132‑1 (MQ=255)                                                                                                                                                                                                            aCCCTGTAGCAGCGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGcc                                >  1:68296/1‑249 (MQ=255)                                                                                                                                                                                                                   agcagcGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACgggc                       <  1:66029/251‑4 (MQ=255)                                                                                                                                                                                                                     cagcGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCg                      >  2:140155/1‑250 (MQ=255)                                                                                                                                                                                                                        cGTGCGGGTC‑AGCACTACG‑CCGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATaa                  <  2:391370/251‑1 (MQ=255)                                                                                                                                                                                                                                 c‑AGCACTACA‑CCGATTGCCAGTGAGAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCtt         >  1:237649/1‑251 (MQ=255)                                                                                                                                                                                                                                         tacgccGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCttttttcc                                                                        >  2:360671/5‑180 (MQ=255)                                                                                                                                                                                                                                         tacgccGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTAc                                                                        <  1:360671/178‑1 (MQ=255)                                                                                                                                                                                                                                          acgccGATTGCCAGTGAAAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGGCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTc  >  2:439504/4‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             CGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTG‑GACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTC‑AGCACTACA‑CCGATTGCCAGTGAGAGCA‑GCAACATCAGGCGTAAAATGTTAGTGGTGTTAT‑CCACCT‑GTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTC  >  NC_000913/4624030‑4624510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑