Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,055	T→C	E21E (GAA→GAG)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,055	0	T	C	100.0%	234.2 / NA	73	E21E (GAA→GAG)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/38);  total (35/38)

CGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAA  >  NC_000913/4623809‑4624297                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ggACGGGGAGATGTTTTTACCGTACCCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCa                                                                                                                                                                                                                                                 <  2:299678/250‑1 (MQ=255) cGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGACCGCGCTGCGTGGCGGGCATTTGGCGAAAATTACACtcctccc                                                                                                                                                                                                                                                 >  1:333514/1‑250 (MQ=255)   aCGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCAcc                                                                                                                                                                                                                                               <  2:288666/251‑1 (MQ=255)       ggAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTCCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCCTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAACAATACACCCCTCCAcccccc                                                                                                                                                                                                                                           >  2:336341/1‑247 (MQ=255)            tggTTTTATCGTACTCCGGCTTGCCAGCCCTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGtt                                                                                                                                                                                                                                       >  1:179621/3‑250 (MQ=255)            tggTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCGAGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGtt                                                                                                                                                                                                                                       >  1:268186/3‑250 (MQ=255)                      gTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAACACGGGt                                                                                                                                                                                                                            >  1:287073/1‑251 (MQ=255)                                 tGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTc                                                                                                                                                                                                                  <  1:35211/250‑1 (MQ=255)                                    cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCCTCTGGCGAAAAATACACTCCTCCACCGCCCGGTTAAACCACGGGTCGTAAGAGTCag                                                                                                                                                                                                                >  2:370269/1‑249 (MQ=255)                                     agccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCACTACTGCGCCGTACCAAGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCATTCCACGGGTTCTGAGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGCGAAACAGAACGCGCTGCGTGGCGGGCATCTGGTGACAAATACACTCCTCCACCGCCAGGTTAAACCACGAGTCGTAAGAGCCagcg                                                                                                                                                                                                              >  2:383810/1‑248 (MQ=255)                                       ccaTCAAGGTAAAGCAGGGATTGCCTAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGGAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGGGTCAGAGATg                                                                                                                                                                                                           >  1:470759/1‑251 (MQ=255)                                              ggcaatgatgggaTTGCCGCGATCGTTGACCCCCGCGTCGCCACCGCTACAGCGCCGTGCCGGGCGGATTTTATGTTCTGCCAGCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGGGTTGGCATTGCGCCAGAGAAACAGACCGCGCTGCGTGGCTGGCATCGGGGGAAAAATCCACTCCTCCTCCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                                                                                                                                                                    <  2:258385/239‑1 (MQ=255)                                                tAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAATTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                                                                                                                                                                  >  1:43765/1‑251 (MQ=255)                                                  aaGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCCCCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAATTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTaa                                                                                                                                                                                                <  2:43765/251‑1 (MQ=255)                                                   aGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCGTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                                                                                               <  2:165984/251‑1 (MQ=255)                                                    gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                                                                                                                                                              >  1:70490/1‑251 (MQ=255)                                                    gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTACTCCACCGCCAGGTTAACCCACGGGTCGTAAGGGTCAGAGCTGCGCAGGCGTAAtg                                                                                                                                                                                              >  2:78316/1‑251 (MQ=255)                                                     cagttATTGCCGCGATCGGTGACCCCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGGCAGAGATGAGCAGGCGTAAtgt                                                                                                                                                                                             <  2:333514/247‑1 (MQ=255)                                                      aGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                                                                                                                                                            <  1:63562/251‑1 (MQ=255)                                                          attgcctgtattgTGGAACACCGCGCCGCCACCGCTACTGCTCCGTGCTAGGCGGGCGTTATCTTCTGCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGTCCAATCACTACCGTGTCGTCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGGTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTTGAc                                                                                                                                                                                         <  2:39523/238‑1 (MQ=255)                                                          aTTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAACACGGGTCGTAAGAGTCAGAGATGAGCAGGAGTAATGTGGAc                                                                                                                                                                                         >  1:100980/1‑250 (MQ=255)                                                            tGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAACCCACGGGTCGTAAGAGTCAGAAATGAGCAGGCGTAATGGGGACAt                                                                                                                                                                                       >  2:320904/1‑250 (MQ=255)                                                            tGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACAt                                                                                                                                                                                       <  2:109812/250‑1 (MQ=255)                                                             gCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGGGGACATa                                                                                                                                                                                      >  1:198195/1‑250 (MQ=255)                                                              acGAGATCGTGGCACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTAATCTTCTTCCATCCGCCGGGGATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGAGCTGCGTGGCGGGCATCTTGCGAAAAATACAATCCTCCACCGCCCGGATAAACCACGGGTAGTAAGAGTTAGAGCTGAGCAGGCGGAACGGGGACATaa                                                                                                                                                                                     >  2:388097/2‑250 (MQ=255)                                                               cGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACg                                                                                                                                                                                   <  1:49457/251‑1 (MQ=255)                                                                gagaTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAGTCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGGCGTTAGAATCAGGGTTGAGCAGGCGGAATGTGGACGTAACg                                                                                                                                                                                   >  2:304369/1‑250 (MQ=255)                                                                gagaTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGa                                                                                                                                                                                  <  1:320904/251‑1 (MQ=255)                                                                 agaTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGCCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACCCTCCTCCACCGCCCGGTTAAACCACGGATCGTAAGAGTAAGAGATGAGCAGGCGTAATGTGGCAATAACggt                                                                                                                                                                                 >  2:276184/1‑249 (MQ=255)                                                                   aTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                                                                                                                                                               <  1:425347/251‑1 (MQ=255)                                                                        ggAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                                                                                          >  1:421456/1‑251 (MQ=255)                                                                           acacCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                                                                                                                                                       <  1:130757/251‑1 (MQ=255)                                                                               cgcgCAGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtag                                                                                                                                                                   <  2:42375/251‑1 (MQ=255)                                                                                 cgccgcCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCTCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                                                                                                                                                                 <  1:437968/251‑1 (MQ=255)                                                                                  gccgccACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                                                                                                                                                                <  1:340556/251‑1 (MQ=255)                                                                                    cgccgccgcTACGGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACTCTCTTTCCACGGGTTCTGCTCGTGACTAATCACTACCGGGTGGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAATATACGCTGCTCCACCGTCAGGTTAAACCACGGGTCGTAAGAGTGAGAGATGTGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                                                                                                                                                              <  1:291798/251‑1 (MQ=255)                                                                                     gccACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATgg                                                                                                                                                             >  1:383654/1‑251 (MQ=255)                                                                                            cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                                                                                          >  1:127905/1‑231 (MQ=255)                                                                                            cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                                                                                          <  2:127905/231‑1 (MQ=255)                                                                                            cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTCGTAATGGGTc                                                                                                                                                          >  2:226123/1‑247 (MQ=255)                                                                                            cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTc                                                                                                                                                          <  1:226123/247‑1 (MQ=255)                                                                                              ccTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtt                                                                                                                                                    <  1:264809/250‑1 (MQ=255)                                                                                                   gccgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc                                                                                                                                               <  1:370269/251‑1 (MQ=255)                                                                                                        gccAGGCGGACGTTATCTTCTTCCCTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGGTAAACCTCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGGAATGGGTCACTCTTTTTGCTCGCt                                                                                                                                          <  2:133151/251‑1 (MQ=255)                                                                                                        gccAGACGGACGTCATCTTCTTCCATCCGCCGGGTATTACACTCTTTCAACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGACAGAGAAACAGAACGCGCTGCGTGCCGGGCATCTGGCGAAAAATACACACCCCCACCGCCAGGTTAAACCCAGGGCCGTCAGAGTCAGAGATGAGAAGCCGTAATGTGGACGAAACGTTTTCCTTTCTTGTCGTCATGGGCCCTTTTTTTTTCTCGCt                                                                                                                                          >  2:406633/1‑251 (MQ=255)                                                                                                            ggCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTttct                                                                                                                                      <  1:109956/251‑1 (MQ=255)                                                                                                               ggACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcc                                                                                                                                   >  1:129932/1‑251 (MQ=255)                                                                                                                gACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcct                                                                                                                                  >  1:13241/1‑251 (MQ=255)                                                                                                                      aTCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCg                                                                                                                            >  2:109060/1‑251 (MQ=255)                                                                                                                       tcttctTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCgg                                                                                                                           >  1:402575/1‑251 (MQ=255)                                                                                                                       tcttctTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCgg                                                                                                                           >  1:424130/1‑251 (MQ=255)                                                                                                                               cATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCTCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGACCGCGCTGCGTGGCGTGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGtt                                                                                                                   <  1:383810/251‑1 (MQ=255)                                                                                                                                aTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt                                                                                                                  <  1:146621/251‑1 (MQ=255)                                                                                                                                       ggggaTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGGAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑g                                                                                                          >  2:403658/5‑251 (MQ=255)                                                                                                                                           aTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGAGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGACAGAGATAAGCAGGCGTAATGTGGACATAAAGATTTACTTTCTTGTCGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑gttct                                                                                                      >  1:314710/1‑247 (MQ=255)                                                                                                                                                      tCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGGACGCGATGCGTGGCGGGCATCTGGCGAAAAATAACCCCCTCCACCGCCCGGTTAAACCACGGGTCGTAGGAGTCAGAGATGAGCAGGCGTAATGTGGACGTACCGATTTCCTTTCTTGTGGTACTGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTACGGCACCGGTTTGATGGCTGGTTAACAGGAgggg                                                                                            >  2:47798/1‑251 (MQ=255)                                                                                                                                                        cACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAgggggg                                                                                         >  1:410054/1‑248 (MQ=255)                                                                                                                                                           gggTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATtg                                                                                      <  2:238165/251‑1 (MQ=255)                                                                                                                                                                  gcgcgcGACCAATCACTGCCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCa                                                                                <  1:188172/250‑1 (MQ=255)                                                                                                                                                                                        gtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTagc                                                          <  1:393212/250‑1 (MQ=255)                                                                                                                                                                                         tCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCACCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCAAGGGTCGTAAGAGTCAGAGTTGGGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGGAGTAATGGGTCACTATTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAAACGGATGGGCGATTGCGGCCCCCGGGTACGTTTATCCtttggca                                                         >  2:116975/1‑244 (MQ=255)                                                                                                                                                                                                 gagcCAGAGAACCAGAACGCGCTGCGTGGCGGGGTTCTGGCGAAAAATACACCCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTGGTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCggg                                                <  2:468380/249‑1 (MQ=255)                                                                                                                                                                                                  cgcCAGCGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt                                                                                                                  >  2:313352/1‑185 (MQ=255)                                                                                                                                                                                                  cgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt                                                                                                                  <  1:313352/185‑1 (MQ=255)                                                                                                                                                                                                         gaAACAGAACGCGCGGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACt                                        <  2:129932/251‑1 (MQ=255)                                                                                                                                                                                                                            ggCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTgaaa                     <  1:199888/251‑3 (MQ=255)                                                                                                                                                                                                                              cGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAagc                   >  1:132392/1‑251 (MQ=255)                                                                                                                                                                                                                                  catctGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAgcagca               <  1:246360/247‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGt    <  1:422683/251‑1 (MQ=255)                                                                                                                                                                                                                                               aTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc                                                                                                                                               >  1:241675/1‑111 (MQ=255)                                                                                                                                                                                                                                               aTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc                                                                                                                                               <  2:241675/111‑1 (MQ=255)                                                                                                                                                                                                                                               aTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGAAATGGGTCACTCTTTTTtc                                                                                                                                               >  1:236114/1‑111 (MQ=255)                                                                                                                                                                                                                                               aTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTGGTAGTAATGGGTCACTCTTTTTtc                                                                                                                                               <  2:236114/111‑1 (MQ=255)                                                                                                                                                                                                                                               aTACACTCCCCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCGCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTaa  <  2:421456/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAA  >  NC_000913/4623809‑4624297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑