Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,624,037 T→C Q27Q (CAA→CAG)  lplA ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,624,0370TC97.1% 197.4 / ‑4.0 68Q27Q (CAA→CAG) lplAlipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (33/33);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGC  >  NC_000913/4623793‑4624280
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
gcgcATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCAGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGa                                                                                                                                                                                                                                                <  1:147779/251‑1 (MQ=255)
                ggACGGGGAGATGTTTTTACCGTACCCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCa                                                                                                                                                                                                                                <  2:299678/250‑1 (MQ=255)
                cGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGACCGCGCTGCGTGGCGGGCATTTGGCGAAAATTACACtcctccc                                                                                                                                                                                                                                >  1:333514/1‑250 (MQ=255)
                  aCGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCAcc                                                                                                                                                                                                                              <  2:288666/251‑1 (MQ=255)
                      ggAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTCCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCCTTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAACAATACACCCCTCCAcccccc                                                                                                                                                                                                                          >  2:336341/1‑247 (MQ=255)
                           tggTTTTATCGTACTCCGGCTTGCCAGCCCTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGtt                                                                                                                                                                                                                      >  1:179621/3‑250 (MQ=255)
                           tggTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCGAGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGtt                                                                                                                                                                                                                      >  1:268186/3‑250 (MQ=255)
                                     gTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAACACGGGt                                                                                                                                                                                                           >  1:287073/1‑251 (MQ=255)
                                                tGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTc                                                                                                                                                                                                 <  1:35211/250‑1 (MQ=255)
                                                   cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCCTCTGGCGAAAAATACACTCCTCCACCGCCCGGTTAAACCACGGGTCGTAAGAGTCag                                                                                                                                                                                               >  2:370269/1‑249 (MQ=255)
                                                    agccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCACTACTGCGCCGTACCAAGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCATTCCACGGGTTCTGAGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGCGAAACAGAACGCGCTGCGTGGCGGGCATCTGGTGACAAATACACTCCTCCACCGCCAGGTTAAACCACGAGTCGTAAGAGCCagcg                                                                                                                                                                                             >  2:383810/1‑248 (MQ=255)
                                                      ccaTCAAGGTAAAGCAGGGATTGCCTAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGGAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGGGTCAGAGATg                                                                                                                                                                                          >  1:470759/1‑251 (MQ=255)
                                                             ggcaatgatgggaTTGCCGCGATCGTTGACCCCCGCGTCGCCACCGCTACAGCGCCGTGCCGGGCGGATTTTATGTTCTGCCAGCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGGGTTGGCATTGCGCCAGAGAAACAGACCGCGCTGCGTGGCTGGCATCGGGGGAAAAATCCACTCCTCCTCCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                                                                                                                                                   <  2:258385/239‑1 (MQ=255)
                                                               tAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAATTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGt                                                                                                                                                                                 >  1:43765/1‑251 (MQ=255)
                                                                 aaGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCCCCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAATTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTaa                                                                                                                                                                               <  2:43765/251‑1 (MQ=255)
                                                                  aGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCGTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAt                                                                                                                                                                              <  2:165984/251‑1 (MQ=255)
                                                                   gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtg                                                                                                                                                                             >  1:70490/1‑251 (MQ=255)
                                                                   gCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTACTCCACCGCCAGGTTAACCCACGGGTCGTAAGGGTCAGAGCTGCGCAGGCGTAAtg                                                                                                                                                                             >  2:78316/1‑251 (MQ=255)
                                                                    cagttATTGCCGCGATCGGTGACCCCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGGCAGAGATGAGCAGGCGTAAtgt                                                                                                                                                                            <  2:333514/247‑1 (MQ=255)
                                                                     aGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgtg                                                                                                                                                                           <  1:63562/251‑1 (MQ=255)
                                                                         attgcctgtattgTGGAACACCGCGCCGCCACCGCTACTGCTCCGTGCTAGGCGGGCGTTATCTTCTGCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGTCCAATCACTACCGTGTCGTCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGGTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTTGAc                                                                                                                                                                        <  2:39523/238‑1 (MQ=255)
                                                                         aTTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAACACGGGTCGTAAGAGTCAGAGATGAGCAGGAGTAATGTGGAc                                                                                                                                                                        >  1:100980/1‑250 (MQ=255)
                                                                           tGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAACCCACGGGTCGTAAGAGTCAGAAATGAGCAGGCGTAATGGGGACAt                                                                                                                                                                      >  2:320904/1‑250 (MQ=255)
                                                                           tGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACAt                                                                                                                                                                      <  2:109812/250‑1 (MQ=255)
                                                                            gCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGGGGACATa                                                                                                                                                                     >  1:198195/1‑250 (MQ=255)
                                                                             acGAGATCGTGGCACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTAATCTTCTTCCATCCGCCGGGGATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGAGCTGCGTGGCGGGCATCTTGCGAAAAATACAATCCTCCACCGCCCGGATAAACCACGGGTAGTAAGAGTTAGAGCTGAGCAGGCGGAACGGGGACATaa                                                                                                                                                                    >  2:388097/2‑250 (MQ=255)
                                                                              cGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACg                                                                                                                                                                  <  1:49457/251‑1 (MQ=255)
                                                                               gagaTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAGTCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGGCGTTAGAATCAGGGTTGAGCAGGCGGAATGTGGACGTAACg                                                                                                                                                                  >  2:304369/1‑250 (MQ=255)
                                                                               gagaTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGa                                                                                                                                                                 <  1:320904/251‑1 (MQ=255)
                                                                                agaTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGCCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACCCTCCTCCACCGCCCGGTTAAACCACGGATCGTAAGAGTAAGAGATGAGCAGGCGTAATGTGGCAATAACggt                                                                                                                                                                >  2:276184/1‑249 (MQ=255)
                                                                                  aTCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                                                                                                                                              <  1:425347/251‑1 (MQ=255)
                                                                                       ggAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                                                                         >  1:421456/1‑251 (MQ=255)
                                                                                          acacCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCtt                                                                                                                                                      <  1:130757/251‑1 (MQ=255)
                                                                                              cgcgCAGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtag                                                                                                                                                  <  2:42375/251‑1 (MQ=255)
                                                                                                cgccgcCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCTCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagta                                                                                                                                                <  1:437968/251‑1 (MQ=255)
                                                                                                 gccgccACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                                                                                                                                               <  1:340556/251‑1 (MQ=255)
                                                                                                   cgccgccgcTACGGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACTCTCTTTCCACGGGTTCTGCTCGTGACTAATCACTACCGGGTGGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAATATACGCTGCTCCACCGTCAGGTTAAACCACGGGTCGTAAGAGTGAGAGATGTGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATg                                                                                                                                             <  1:291798/251‑1 (MQ=255)
                                                                                                    gccACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATgg                                                                                                                                            >  1:383654/1‑251 (MQ=255)
                                                                                                           cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                                                                         <  2:127905/231‑1 (MQ=255)
                                                                                                           cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                                                                                         >  1:127905/1‑231 (MQ=255)
                                                                                                           cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTCGTAATGGGTc                                                                                                                                         >  2:226123/1‑247 (MQ=255)
                                                                                                           cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTc                                                                                                                                         <  1:226123/247‑1 (MQ=255)
                                                                                                             ccTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtt                                                                                                                                   <  1:264809/250‑1 (MQ=255)
                                                                                                                  gccgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc                                                                                                                              <  1:370269/251‑1 (MQ=255)
                                                                                                                       gccAGGCGGACGTTATCTTCTTCCCTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGGTAAACCTCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGGAATGGGTCACTCTTTTTGCTCGCt                                                                                                                         <  2:133151/251‑1 (MQ=255)
                                                                                                                       gccAGACGGACGTCATCTTCTTCCATCCGCCGGGTATTACACTCTTTCAACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGACAGAGAAACAGAACGCGCTGCGTGCCGGGCATCTGGCGAAAAATACACACCCCCACCGCCAGGTTAAACCCAGGGCCGTCAGAGTCAGAGATGAGAAGCCGTAATGTGGACGAAACGTTTTCCTTTCTTGTCGTCATGGGCCCTTTTTTTTTCTCGCt                                                                                                                         >  2:406633/1‑251 (MQ=255)
                                                                                                                           ggCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTttct                                                                                                                     <  1:109956/251‑1 (MQ=255)
                                                                                                                              ggACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcc                                                                                                                  >  1:129932/1‑251 (MQ=255)
                                                                                                                               gACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcct                                                                                                                 >  1:13241/1‑251 (MQ=255)
                                                                                                                                     aTCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCg                                                                                                           >  2:109060/1‑251 (MQ=255)
                                                                                                                                      tcttctTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCgg                                                                                                          >  1:402575/1‑251 (MQ=255)
                                                                                                                                      tcttctTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCgg                                                                                                          >  1:424130/1‑251 (MQ=255)
                                                                                                                                              cATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCTCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGACCGCGCTGCGTGGCGTGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGtt                                                                                                  <  1:383810/251‑1 (MQ=255)
                                                                                                                                               aTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt                                                                                                 <  1:146621/251‑1 (MQ=255)
                                                                                                                                                      ggggaTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGGAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑g                                                                                         >  2:403658/5‑251 (MQ=255)
                                                                                                                                                          aTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGAGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGACAGAGATAAGCAGGCGTAATGTGGACATAAAGATTTACTTTCTTGTCGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑gttct                                                                                     >  1:314710/1‑247 (MQ=255)
                                                                                                                                                                     tCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGGACGCGATGCGTGGCGGGCATCTGGCGAAAAATAACCCCCTCCACCGCCCGGTTAAACCACGGGTCGTAGGAGTCAGAGATGAGCAGGCGTAATGTGGACGTACCGATTTCCTTTCTTGTGGTACTGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTACGGCACCGGTTTGATGGCTGGTTAACAGGAgggg                                                                           >  2:47798/1‑251 (MQ=255)
                                                                                                                                                                       cACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAgggggg                                                                        >  1:410054/1‑248 (MQ=255)
                                                                                                                                                                          gggTTCTGCGCGCGACCAATCCCTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATtg                                                                     <  2:238165/251‑1 (MQ=255)
                                                                                                                                                                                 gcgcgcGACCAATCACTGCCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCa                                                               <  1:188172/250‑1 (MQ=255)
                                                                                                                                                                                                       gtCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTagc                                         <  1:393212/250‑1 (MQ=255)
                                                                                                                                                                                                        tCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCACCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCAAGGGTCGTAAGAGTCAGAGTTGGGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGGAGTAATGGGTCACTATTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAAACGGATGGGCGATTGCGGCCCCCGGGTACGTTTATCCtttggca                                        >  2:116975/1‑244 (MQ=255)
                                                                                                                                                                                                                gagcCAGAGAACCAGAACGCGCTGCGTGGCGGGGTTCTGGCGAAAAATACACCCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTGGTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCggg                               <  2:468380/249‑1 (MQ=255)
                                                                                                                                                                                                                 cgcCAGCGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt                                                                                                 >  2:313352/1‑185 (MQ=255)
                                                                                                                                                                                                                 cgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt                                                                                                 <  1:313352/185‑1 (MQ=255)
                                                                                                                                                                                                                        gaAACAGAACGCGCGGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACt                       <  2:129932/251‑1 (MQ=255)
                                                                                                                                                                                                                                           ggCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTgaaa    <  1:199888/251‑3 (MQ=255)
                                                                                                                                                                                                                                             cGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAagc  >  1:132392/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
GCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGC‑GGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGC  >  NC_000913/4623793‑4624280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑