BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,763	C→T	I387I (ATC→ATT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,763	0	C	T	97.4%	117.7 / ‑4.2	39	I387I (ATC→ATT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (13/25); total (14/25)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

CTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCTTGAAGATGCCCG  >  NC_000913/4620514‑4620995

cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGATCACGGTTGCGATCCGACCTGGAACGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAACGTAAAACCGGGATCACTGGTTCATCGTGAAACCTTCGCGGATATCg                                                                                                                                                                                                                                         >  2:210759/1‑251 (MQ=255)

           ccTGGGGCCACCTCCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGTCCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGGTGGGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg                                                                                                                                                                                                                              <  2:109635/251‑1 (MQ=255)

            cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAcgcttg                                                                                                                                                                                                                             >  2:295715/1‑246 (MQ=255)

            cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTgg                                                                                                                                                                                                                             >  1:172214/1‑251 (MQ=255)

                       gTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACCTCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGCCCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACATTCGCGGATATTGGACAGCCTCTGGCAAAATAtttt                                                                                                                                                                                                                  >  1:454689/1‑251 (MQ=255)

                        gcgcgACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTg                                                                                                                                                                                                                 <  1:104009/250‑1 (MQ=255)

                                           gctgcgGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAat                                                                                                                                                                                               <  2:111667/247‑1 (MQ=255)

                                                     tGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGat                                                                                                                                                                                                      <  1:446364/233‑1 (MQ=255)

                                                     tGGAGCTGTTCGACAGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCCCCGTGAAACCTTCGCGGATATTGGTCAGCCTCTGGCAAAATATTTTGGTACTTCTGat                                                                                                                                                                                                      >  2:446364/1‑233 (MQ=255)

                                                     gggagcTGTTCGACCGCCGTCTGCCGGTGCTGATGTGTCTGCTGCGCGATGACGTCATCCTGATCCTCACTGCTGGCCAAGGGTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCTCCGTGAAACCTTCGCGGATATTGGGCAGACTCGGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGc                                                                                                                                                                                     <  2:296977/245‑1 (MQ=255)

                                                       gagcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCAt                                                                                                                                                                                  <  1:295715/248‑1 (MQ=255)

                                                          cTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCCAAATATTTTGGTACTTCTGATATGGAATATGACAAAGCCATGtt                                                                                                                                                                               >  2:155883/1‑251 (MQ=255)

                                                                              ggAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttg                                                                                                                                                           <  2:258980‑M1/251‑16 (MQ=255)

                                                                                     aTGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgcccc                                                                                                                                                    >  2:469188‑M1/1‑229 (MQ=255)

                                                                                       gTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCGCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgctgcttgatgccctc                                                                                                                                                   <  1:36075‑M1/250‑24 (MQ=255)

                                                                                                       gacgacATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGACAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctccc                                                                                                                                   <  1:155883‑M1/250‑40 (MQ=255)

                                                                                                           acagcctgatCCGCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagg                                                                                                                              <  2:172214‑M1/247‑45 (MQ=255)

                                                                                                                    tCCTCACTGCTGACCCCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGTATATTGGTCAGACTCTGGCAAAATATTTTGGTACTGCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacatggagagggt                                                                                                                      <  2:454689‑M1/250‑53 (MQ=255)

                                                                                                                            gcgtgccacggtTGGGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGTGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTTGTCAGACTCGGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagatggtgaaaacatg                                                                                                             <  2:83605‑M1/246‑62 (MQ=255)

                                                                                                                               ggcctcggtggtgatccgacctggaCCGGTACGGACCACACTCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGGTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacaggtagagggtgaaaacatgtt                                                                                                           <  2:414099‑M1/238‑64 (MQ=255)

                                                                                                                                aCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCaaaa                                                                                                                                                                                                                        >  2:100898/1‑140 (MQ=255)

                                                                                                                                aCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCaaaa                                                                                                                                                                                                                        <  1:100898/140‑1 (MQ=255)

                                                                                                                                                       aCCGGTACTGACCACACGCGGGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccc                                                                                  <  2:328637‑M1/251‑89 (MQ=255)

                                                                                                                                                        ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttggtgccctcaccccggccctctcccaccgggagagggtgaaaacatgtttataaggataaaaaaatgggtacccc                                                                                 >  2:392486‑M1/1‑162 (MQ=255)

                                                                                                                                                                gACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacat                                                                           <  1:79883‑M1/249‑96 (MQ=255)

                                                                                                                                                                           tGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggagaaaacatgttggttagggtaaaacaaaggctacccccaacattaaagcagaaatt                                                              >  1:31792‑M1/1‑143 (MQ=255)

                                                                                                                                                                                      cGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCGTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgc                                                   <  2:436197‑M1/251‑120 (MQ=255)

                                                                                                                                                                                          aCTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaac                                                                                                                <  2:22993‑M1/186‑59 (MQ=255)

                                                                                                                                                                                             ggtttacggcccgaaaGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgta                                            <  1:222147‑M1/244‑127 (MQ=255)

                                                                                                                                                                                                            aGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggc                             <  1:178070‑M1/251‑142 (MQ=255)

                                                                                                                                                                                                            aGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggc                             <  1:418095‑M1/251‑142 (MQ=255)

                                                                                                                                                                                                                         tCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatggcctcaccccggccctctcccacagggagagggtgaaaacatgttggtaagggtaaaaaaatggctaccccacacattaatgcagaaatgggcgatttcgctgaagtagatttgatgccaggcgacccgctgcgtg                >  1:277862‑M1/1‑97 (MQ=255)

                                                                                                                                                                                                                         tCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctccccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacaccttaatgcaaaaatgggcgatttcgctgacgg                                             >  2:108491‑M1/1‑97 (MQ=255)

                                                                                                                                                                                                                         tCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgt                                             <  1:108491‑M1/222‑126 (MQ=255)

                                                                                                                                                                                                                               ggTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggggaaaacatgttgataaggataaaacaatggctaccccacacattaaagcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacccgctgcgtgcgaggg          >  2:11688‑M1/1‑91 (MQ=255)

                                                                                                                                                                                                                                  caccgtgAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacccgctgcgtgcgaagtat        <  1:469188‑M1/247‑163 (MQ=255)

                                                                                                                                                                                                                                   accgtGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacc                         <  2:128286‑M1/230‑146 (MQ=255)

                                                                                                                                                                                                                                   accgtGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcacccccgccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagtttttatgcccggcgacc                         >  1:128286‑M1/3‑87 (MQ=255)

                                                                                                                                                                                                                                        gAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTTCTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgattgcgctgacgtagttttgatgccaggcgacccgctgcgtgcgaagtattttgct  <  1:93739‑M1/250‑169 (MQ=255)

CTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCTTGAAGATGCCCG  >  NC_000913/4620514‑4620995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`