BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,742	T→C	H380H (CAT→CAC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,742	0	T	C	97.6%	119.1 / ‑4.4	41	H380H (CAT→CAC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (13/27); total (14/27)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

CACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCTTGAA  >  NC_000913/4620499‑4620987

cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGaa                                                                                                                                                                                                                                                 <  1:346761/250‑1 (MQ=255)

     cctgcGTTGGCTTCGACTCTGCCTGGGGCCACCGTCGCGGCGTCGCCGGTTAGGCTGCGGGTCTGGGGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTTCTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCACTGTGTCACCGTGAAACCTTc                                                                                                                                                                                                                                           <  2:26583/247‑1 (MQ=255)

         cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgcg                                                                                                                                                                                                                                        <  1:135919/250‑1 (MQ=255)

         cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCgg                                                                                                                                                                                                                                       <  1:242039/251‑1 (MQ=255)

               cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGATCACGGTTGCGATCCGACCTGGAACGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAACGTAAAACCGGGATCACTGGTTCATCGTGAAACCTTCGCGGATATCg                                                                                                                                                                                                                                 >  2:210759/1‑251 (MQ=255)

                  cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGatattggt                                                                                                                                                                                                                               <  1:150484/250‑5 (MQ=255)

                          ccTGGGGCCACCTCCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGTCCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGGTGGGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg                                                                                                                                                                                                                      <  2:109635/251‑1 (MQ=255)

                           cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAcgcttg                                                                                                                                                                                                                     >  2:295715/1‑246 (MQ=255)

                           cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTgg                                                                                                                                                                                                                     >  1:172214/1‑251 (MQ=255)

                            tGGGGCCACAGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCACACTGCTGCCCACGTTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACTGCACGAAAGTAAAACAGGTCTCCCTGGCACACCGTGAAACCCTCGCGGatattggttagactcatgc                                                                                                                                                                                                                    >  2:259204/1‑236 (MQ=255)

                                      gTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACCTCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGCCCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACATTCGCGGATATTGGACAGCCTCTGGCAAAATAtttt                                                                                                                                                                                                          >  1:454689/1‑251 (MQ=255)

                                       gcgcgACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTg                                                                                                                                                                                                         <  1:104009/250‑1 (MQ=255)

                                                          gctgcgGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAat                                                                                                                                                                                       <  2:111667/247‑1 (MQ=255)

                                                                    tGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGat                                                                                                                                                                                              <  1:446364/233‑1 (MQ=255)

                                                                    tGGAGCTGTTCGACAGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCCCCGTGAAACCTTCGCGGATATTGGTCAGCCTCTGGCAAAATATTTTGGTACTTCTGat                                                                                                                                                                                              >  2:446364/1‑233 (MQ=255)

                                                                    gggagcTGTTCGACCGCCGTCTGCCGGTGCTGATGTGTCTGCTGCGCGATGACGTCATCCTGATCCTCACTGCTGGCCAAGGGTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCTCCGTGAAACCTTCGCGGATATTGGGCAGACTCGGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGc                                                                                                                                                                             <  2:296977/245‑1 (MQ=255)

                                                                      gagcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCAt                                                                                                                                                                          <  1:295715/248‑1 (MQ=255)

                                                                         cTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCCAAATATTTTGGTACTTCTGATATGGAATATGACAAAGCCATGtt                                                                                                                                                                       >  2:155883/1‑251 (MQ=255)

                                                                                             ggAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttg                                                                                                                                                   <  2:258980‑M1/251‑16 (MQ=255)

                                                                                                    aTGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgcccc                                                                                                                                            >  2:469188‑M1/1‑229 (MQ=255)

                                                                                                      gTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCGCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgctgcttgatgccctc                                                                                                                                           <  1:36075‑M1/250‑24 (MQ=255)

                                                                                                                      gacgacATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGACAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctccc                                                                                                                           <  1:155883‑M1/250‑40 (MQ=255)

                                                                                                                          acagcctgatCCGCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagg                                                                                                                      <  2:172214‑M1/247‑45 (MQ=255)

                                                                                                                                   tCCTCACTGCTGACCCCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGTATATTGGTCAGACTCTGGCAAAATATTTTGGTACTGCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacatggagagggt                                                                                                              <  2:454689‑M1/250‑53 (MQ=255)

                                                                                                                                           gcgtgccacggtTGGGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGTGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTTGTCAGACTCGGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagatggtgaaaacatg                                                                                                     <  2:83605‑M1/246‑62 (MQ=255)

                                                                                                                                              ggcctcggtggtgatccgacctggaCCGGTACGGACCACACTCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGGTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacaggtagagggtgaaaacatgtt                                                                                                   <  2:414099‑M1/238‑64 (MQ=255)

                                                                                                                                               aCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCaaaa                                                                                                                                                                                                                >  2:100898/1‑140 (MQ=255)

                                                                                                                                               aCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCaaaa                                                                                                                                                                                                                <  1:100898/140‑1 (MQ=255)

                                                                                                                                                                      aCCGGTACTGACCACACGCGGGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccc                                                                          <  2:328637‑M1/251‑89 (MQ=255)

                                                                                                                                                                       ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttggtgccctcaccccggccctctcccaccgggagagggtgaaaacatgtttataaggataaaaaaatgggtacccc                                                                         >  2:392486‑M1/1‑162 (MQ=255)

                                                                                                                                                                               gACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacat                                                                   <  1:79883‑M1/249‑96 (MQ=255)

                                                                                                                                                                                          tGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggagaaaacatgttggttagggtaaaacaaaggctacccccaacattaaagcagaaatt                                                      >  1:31792‑M1/1‑143 (MQ=255)

                                                                                                                                                                                                     cGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCGTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgc                                           <  2:436197‑M1/251‑120 (MQ=255)

                                                                                                                                                                                                         aCTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaac                                                                                                        <  2:22993‑M1/186‑59 (MQ=255)

                                                                                                                                                                                                            ggtttacggcccgaaaGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgta                                    <  1:222147‑M1/244‑127 (MQ=255)

                                                                                                                                                                                                                           aGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggc                     <  1:418095‑M1/251‑142 (MQ=255)

                                                                                                                                                                                                                           aGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgtagttttgatgccaggc                     <  1:178070‑M1/251‑142 (MQ=255)

                                                                                                                                                                                                                                        tCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatggcctcaccccggccctctcccacagggagagggtgaaaacatgttggtaagggtaaaaaaatggctaccccacacattaatgcagaaatgggcgatttcgctgaagtagatttgatgccaggcgacccgctgcgtg        >  1:277862‑M1/1‑97 (MQ=255)

                                                                                                                                                                                                                                        tCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctccccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacaccttaatgcaaaaatgggcgatttcgctgacgg                                     >  2:108491‑M1/1‑97 (MQ=255)

                                                                                                                                                                                                                                        tCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgctgacgt                                     <  1:108491‑M1/222‑126 (MQ=255)

                                                                                                                                                                                                                                              ggTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggggaaaacatgttgataaggataaaacaatggctaccccacacattaaagcagaaatgggcgatttcgctgacgtagttttgatgccaggcgacccgctgcgtgcgaggg  >  2:11688‑M1/1‑91 (MQ=255)

CACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCGACCCGCTGCGTGCGAAGTATATTGCTGAAACTTTCCTTGAA  >  NC_000913/4620499‑4620987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`