Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,706	A→T	V368V (GTA→GTT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,706	0	A	T	97.7%	140.1 / ‑3.7	44	V368V (GTA→GTT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (15/28);  total (16/28)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

AGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCG  >  NC_000913/4620463‑4620946                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  agagATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGcc                                                                                                                                                                                                                                            >  1:436197/1‑251 (MQ=255)         aagaagCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTa                                                                                                                                                                                                                                    <  2:417186/251‑1 (MQ=255)            aagCGGGTTATAACCCCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaaaa                                                                                                                                                                                                                                 <  1:321383/251‑1 (MQ=255)                    gctAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACCTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGcac                                                                                                                                                                                                                         >  2:46224/3‑249 (MQ=255)                           ccATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGt                                                                                                                                                                                                                 <  1:312130/251‑1 (MQ=255)                             aTCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTcc                                                                                                                                                                                                               >  2:79883/1‑250 (MQ=255)                               cGTCTTCACCAATGTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCacc                                                                                                                                                                                                             <  1:222263/251‑3 (MQ=255)                                     cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGaa                                                                                                                                                                                                        <  1:346761/250‑1 (MQ=255)                                     cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGAAGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGGCCACACGCGTGACCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGC‑CACCTGGGTCacagtgca                                                                                                                                                                                                        >  2:163195/1‑243 (MQ=255)                                          cctgcGTTGGCTTCGACTCTGCCTGGGGCCACCGTCGCGGCGTCGCCGGTTAGGCTGCGGGTCTGGGGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTTCTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGTGTCACCGTGAAACCTTc                                                                                                                                                                                                  <  2:26583/247‑1 (MQ=255)                                              cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTcgcg                                                                                                                                                                                               <  1:135919/250‑1 (MQ=255)                                              cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCgg                                                                                                                                                                                              <  1:242039/251‑1 (MQ=255)                                                    cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGATCACGGTTGCGATCCGACCTGGAACGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAACGTAAAACCGGGATCAC‑TGGTTCATCGTGAAACCTTCGCGGATATCg                                                                                                                                                                                        >  2:210759/1‑251 (MQ=255)                                                       cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGatattggt                                                                                                                                                                                      <  1:150484/250‑5 (MQ=255)                                                               ccTGGGGCCACCTCCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGTCCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGGTGGGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg                                                                                                                                                                             <  2:109635/251‑1 (MQ=255)                                                                cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAcgcttg                                                                                                                                                                            >  2:295715/1‑246 (MQ=255)                                                                cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTgg                                                                                                                                                                            >  1:172214/1‑251 (MQ=255)                                                                 tGGGGCCACAGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCACACTGCTGCCCACGTTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACTGCACGAAAGTAAAACAGGTCTCCC‑TGGCACACCGTGAAACCCTCGCGGatattggttagactcatgc                                                                                                                                                                           >  2:259204/1‑236 (MQ=255)                                                                           gTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACCTCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGCCCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACATTCGCGGATATTGGACAGCCTCTGGCAAAATAtttt                                                                                                                                                                 >  1:454689/1‑251 (MQ=255)                                                                            gcgcgACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTg                                                                                                                                                                <  1:104009/250‑1 (MQ=255)                                                                                               gctgcgGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAat                                                                                                                                              <  2:111667/247‑1 (MQ=255)                                                                                                         tGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGat                                                                                                                                                     <  1:446364/233‑1 (MQ=255)                                                                                                         tGGAGCTGTTCGACAGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCCCCGTGAAACCTTCGCGGATATTGGTCAGCCTCTGGCAAAATATTTTGGTACTTCTGat                                                                                                                                                     >  2:446364/1‑233 (MQ=255)                                                                                                         gggagcTGTTCGACCGCCGTCTGCCGGTGCTGATGTGTCTGCTGCGCGATGACGTCATCCTGATCCTCACTGCTGGCCAAGGGTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCTCCGTGAAACCTTCGCGGATATTGGGCAGACTCGGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGc                                                                                                                                    <  2:296977/245‑1 (MQ=255)                                                                                                           gagcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCAt                                                                                                                                 <  1:295715/248‑1 (MQ=255)                                                                                                             gcTGTTCGACCGCCGTCTTCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAcc                                                                                                                                                                                                                               <  1:147678/155‑1 (MQ=255)                                                                                                             gcTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGCCCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAcc                                                                                                                                                                                                                               >  2:147678/2‑156 (MQ=255)                                                                                                              cTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCCAAATATTTTGGTACTTCTGATATGGAATATGACAAAGCCATGtt                                                                                                                              >  2:155883/1‑251 (MQ=255)                                                                                                                                  ggAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttg                                                                                                          <  2:258980‑M1/251‑16 (MQ=255)                                                                                                                                         aTGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgcccc                                                                                                   >  2:469188‑M1/1‑229 (MQ=255)                                                                                                                                           gTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCGCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgctgcttgatgccctc                                                                                                  <  1:36075‑M1/250‑24 (MQ=255)                                                                                                                                                           gacgacATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGACAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctccc                                                                                  <  1:155883‑M1/250‑40 (MQ=255)                                                                                                                                                               acagcctgatCCGCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagg                                                                             <  2:172214‑M1/247‑45 (MQ=255)                                                                                                                                                                        tCCTCACTGCTGACCCCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGTATATTGGTCAGACTCTGGCAAAATATTTTGGTACTGCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacatggagagggt                                                                     <  2:454689‑M1/250‑53 (MQ=255)                                                                                                                                                                                gcgtgccacggtTGGGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGTGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTTGTCAGACTCGGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagatggtgaaaacatg                                                            <  2:83605‑M1/246‑62 (MQ=255)                                                                                                                                                                                   ggcctcggtggtgatccgacctggaCCGGTACGGACCACACTCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGGTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacaggtagagggtgaaaacatgtt                                                          <  2:414099‑M1/238‑64 (MQ=255)                                                                                                                                                                                    aCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCaaaa                                                                                                                                                                       >  2:100898/1‑140 (MQ=255)                                                                                                                                                                                    aCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCaaaa                                                                                                                                                                       <  1:100898/140‑1 (MQ=255)                                                                                                                                                                                                           aCCGGTACTGACCACACGCGGGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccc                                 <  2:328637‑M1/251‑89 (MQ=255)                                                                                                                                                                                                            ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttggtgccctcaccccggccctctcccaccgggagagggtgaaaacatgtttataaggataaaaaaatgggtacccc                                >  2:392486‑M1/1‑162 (MQ=255)                                                                                                                                                                                                                    gACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacat                          <  1:79883‑M1/249‑96 (MQ=255)                                                                                                                                                                                                                               tGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggagaaaacatgttggttagggtaaaacaaaggctacccccaacattaaagcagaaatt             >  1:31792‑M1/1‑143 (MQ=255)                                                                                                                                                                                                                                          cGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCGTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaacatgttgataaggataaaacaatggctaccccacacattaatgcagaaatgggcgatttcgc  <  2:436197‑M1/251‑120 (MQ=255)                                                                                                                                                                                                                                              aCTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagagggtgaaaac                                                               <  2:22993‑M1/186‑59 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  AGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACATTAATGCAGAAATGGGCGATTTCGCTGACGTAGTTTTGATGCCAGGCG  >  NC_000913/4620463‑4620946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑