Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,571	A→G	E323E (GAA→GAG)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,571	0	A	G	98.3%	186.8 / NA	60	E323E (GAA→GAG)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (22/37);  total (23/37)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAAT  >  NC_000913/4620331‑4620806                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              gcAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGAcc                                                                                                                                                                                                                                     <  1:363452/251‑1 (MQ=255)          ggTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACCTCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGc                                                                                                                                                                                                                            >  1:414099/1‑251 (MQ=255)                 gaAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCt                                                                                                                                                                                                                     <  1:483203/251‑1 (MQ=255)                     aacacgttcAGGGGGTTTCTGTCGGTAAAATTGCAGGCATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATTAAAGAAGCGGGTGAGAACACCAACGTCTTCACCAACTGCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGCCCGCCGGCTGCCGTAGCTGATg                                                                                                                                                                                                                 <  2:408426/243‑1 (MQ=255)                          ggtcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTctct                                                                                                                                                                                                            <  1:83539/248‑1 (MQ=255)                          ggtcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGAGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAACGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCTCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGTCGGCCGGAGCTGTTGActct                                                                                                                                                                                                            >  2:69846/4‑251 (MQ=255)                           ggcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCGGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCAGCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTctg                                                                                                                                                                                                           <  1:325075/249‑1 (MQ=255)                                         gTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTAGCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATgacg                                                                                                                                                                                              <  2:35219/250‑1 (MQ=255)                                            ggTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACAt                                                                                                                                                                                          <  1:281425/251‑1 (MQ=255)                                                   ttGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCGGCGCGATGCCGACCTCCTgctc                                                                                                                                                                                   >  2:104009/1‑248 (MQ=255)                                                       agACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTc                                                                                                                                                                                >  1:417186/2‑250 (MQ=255)                                                         aCATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCAct                                                                                                                                                                             <  1:418641/251‑2 (MQ=255)                                                           aTCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCActgc                                                                                                                                                                           >  2:178070/1‑248 (MQ=255)                                                               aCGCCAACTGCGGTATCACCCAGAAAGTGAAAGCAACTGGCCTGGACGCGCTTTTTGACGCCACCATCAAAGCGATGAAAGTAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCATCGTCGCAGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCACTGCTGAGCGAGGACGACATCCTGATTCTCActgaggac                                                                                                                                                                       >  2:9445/1‑244 (MQ=255)                                                                cGCCAAGTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACTATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACGCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGAcc                                                                                                                                                                      <  2:353786/251‑1 (MQ=255)                                                                  ccAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCAc                                                                                                                                                                    <  1:273171/251‑1 (MQ=255)                                                                     aCTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTGGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGt                                                                                                                                                                 <  1:335448/251‑1 (MQ=255)                                                                      cTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGtt                                                                                                                                                                >  1:258980/1‑251 (MQ=255)                                                                           gtctCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGACTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGGCCACGGTTCTGCTGCGGGTCTGGCGCTGTTCGGCCGCCGTCTGCCGGAGCTGCTGTCTCTTCTGCGCGATTGCGCCATCCTGAACCTCAATGATGACCACGGTTGTGA‑t                                                                                                                                                          >  2:342077/4‑251 (MQ=255)                                                                             aTCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑Tc                                                                                                                                                         <  2:129709/250‑1 (MQ=255)                                                                               cACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGtc                                                                                                                                                                                                               <  2:448162/195‑1 (MQ=255)                                                                               cACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGtc                                                                                                                                                                                                               >  1:448162/1‑195 (MQ=255)                                                                                 ccAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGAGAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCGGGTCGACCGCCGTCTGCCGGAGCGGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGAcc                                                                                                                                                    <  2:100455/251‑1 (MQ=255)                                                                                        agtgaaagcacacggCCTGGACGAGCTGTTTTACGCAACCATCAAAGAGATGAAAGAAGCGGGTGAGAACACCATCGTCTTCACCAACTTCGTTGACGTCGACTCTTCCTGGGGCCACCGTCGCGTCGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCg                                                                                                                                             <  2:15262/238‑1 (MQ=255)                                                                                             aatcaaCTGGCCTGGACGCGCTGTTTGACGCCTCCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTTCCCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGTGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACt                                                                                                                                        <  1:55017/246‑1 (MQ=255)                                                                                                 aacgggCCGGGACTCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGTGTGATAACACCCTCGTCTTCACCATCGTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGAcc                                                                                                                                    <  2:313040/247‑1 (MQ=255)                                                                                                  aCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACca                                                                                                                                   <  1:420027/251‑1 (MQ=255)                                                                                                        cTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGCTCCTCAAAGCTGACCAAGGATG‑GAGTCCGACATGGACCGGCACTGGCCACAcgcg                                                                                                                             >  2:105403/1‑251 (MQ=255)                                                                                                                   gTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTc                                                                                                                  <  1:136298/251‑1 (MQ=255)                                                                                                                      tgccGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACCCCATCGTCTGCCCCTACGTCGTTGACTTCGACTCTTCCTGTGGCCTCCGTCGCGACGTCGCCGTTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGGCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCg                                                                                                                <  2:86987/247‑1 (MQ=255)                                                                                                                           cccccctCAAATAGATGAAAGAAGCGGGGGATAACACCAGTGTCGTCACCAACCTCGTTGACATCGACTCTTCCGGGGGCTACCGTCGCGGCGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGTCCGGTACTGACCACACGCGTGAacagatcggaagatct                                                                                                          <  2:136303/245‑14 (MQ=255)                                                                                                                            caccaTCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGCTGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGC‑TCCGACCTGGACCGGTACTGCCCACACGCGTGAACACATTACGGTACTgg                                                                                                         >  1:26583/1‑251 (MQ=255)                                                                                                                                  cAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttac                                                                                                    <  1:327556/250‑5 (MQ=255)                                                                                                                                    aaGAGATGAAAGAAGCGGGTGATAACACCTTCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacggc                                                                                                 <  1:332951/251‑8 (MQ=255)                                                                                                                                    aaGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTACCGGAGCTGATGTCTCTGCTGCGCGATGGAGACATCCTGATCCTCACTGCTGACACCGGTTGTGA‑TCCGACCTGGACCGGTCCTGACCACACGCGTGAACAAAGTCAGGTACTGGtttacggc                                                                                                 >  2:459572/1‑244 (MQ=255)                                                                                                                                     agagATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGcc                                                                                                >  1:436197/1‑251 (MQ=255)                                                                                                                                             aagaagCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTa                                                                                        <  2:417186/251‑1 (MQ=255)                                                                                                                                                aagCGGGTTATAACCCCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaaaa                                                                                     <  1:321383/251‑1 (MQ=255)                                                                                                                                                        gctAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACCTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGcac                                                                             >  2:46224/3‑249 (MQ=255)                                                                                                                                                               ccATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGt                                                                     <  1:312130/251‑1 (MQ=255)                                                                                                                                                                 aTCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTcc                                                                   >  2:79883/1‑250 (MQ=255)                                                                                                                                                                   cGTCTTCACCAATGTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCacc                                                                 <  1:222263/251‑3 (MQ=255)                                                                                                                                                                         cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGaa                                                            <  1:346761/250‑1 (MQ=255)                                                                                                                                                                         cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGAAGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGGCCACACGCGTGACCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGC‑CACCTGGGTCacagtgca                                                            >  2:163195/1‑243 (MQ=255)                                                                                                                                                                              cctgcGTTGGCTTCGACTCTGCCTGGGGCCACCGTCGCGGCGTCGCCGGTTAGGCTGCGGGTCTGGGGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTTCTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGTGTCACCGTGAAACCTTc                                                      <  2:26583/247‑1 (MQ=255)                                                                                                                                                                                  cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTcgcg                                                   <  1:135919/250‑1 (MQ=255)                                                                                                                                                                                  cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCgg                                                  <  1:242039/251‑1 (MQ=255)                                                                                                                                                                                        cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGATCACGGTTGCGA‑TCCGACCTGGAACGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAACGTAAAACCGGGATCAC‑TGGTTCATCGTGAAACCTTCGCGGATATCg                                            >  2:210759/1‑251 (MQ=255)                                                                                                                                                                                           cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGatattggt                                          <  1:150484/250‑5 (MQ=255)                                                                                                                                                                                                   ccTGGGGCCACCTCCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGTCCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGGTGGGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg                                 <  2:109635/251‑1 (MQ=255)                                                                                                                                                                                                    cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAcgcttg                                >  2:295715/1‑246 (MQ=255)                                                                                                                                                                                                    cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTgg                                >  1:172214/1‑251 (MQ=255)                                                                                                                                                                                                     tGGGGCCACAGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCACACTGCTGCCCACGTTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACTGCACGAAAGTAAAACAGGTCTCCC‑TGGCACACCGTGAAACCCTCGCGGatattggttagactcatgc                               >  2:259204/1‑236 (MQ=255)                                                                                                                                                                                                         gCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTa                                                                                                             <  1:61594/170‑1 (MQ=255)                                                                                                                                                                                                         gCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTa                                                                                                             >  2:61594/1‑170 (MQ=255)                                                                                                                                                                                                               gTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACCTCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGCCCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACATTCGCGGATATTGGACAGCCTCTGGCAAAATAtttt                     >  1:454689/1‑251 (MQ=255)                                                                                                                                                                                                                gcgcgACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTg                    <  1:104009/250‑1 (MQ=255)                                                                                                                                                                                                                                   gctgcgGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAat  <  2:111667/247‑1 (MQ=255)                                                                                                                                                                                                                                             tGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGat         <  1:446364/233‑1 (MQ=255)                                                                                                                                                                                                                                             tGGAGCTGTTCGACAGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCCCCGTGAAACCTTCGCGGATATTGGTCAGCCTCTGGCAAAATATTTTGGTACTTCTGat         >  2:446364/1‑233 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              GCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAAT  >  NC_000913/4620331‑4620806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑