Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,559	C→T	A319A (GCC→GCT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,559	0	C	T	98.3%	191.7 / ‑6.7	60	A319A (GCC→GCT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (22/37);  total (23/37)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTG  >  NC_000913/4620313‑4620788                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        gccAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCgg                                                                                                                                                                                                                                     >  1:328637/1‑251 (MQ=255)    aGCACCGACCGTGCTGCAGAAACTTGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTc                                                                                                                                                                                                                                  <  1:51825/251‑1 (MQ=255)        ccgaccgTGCTGAAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCTCCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGTTTATGCTTCGGGTCTGGa                                                                                                                                                                                                                              <  2:46873/251‑1 (MQ=255)                   gcAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGAcc                                                                                                                                                                                                                   <  1:363452/251‑1 (MQ=255)                            ggTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACCTCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGc                                                                                                                                                                                                          >  1:414099/1‑251 (MQ=255)                                   gaAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCt                                                                                                                                                                                                   <  1:483203/251‑1 (MQ=255)                                       aacacgttcAGGGGGTTTCTGTCGGTAAAATTGCAGGCATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATTAAAGAAGCGGGTGAGAACACCAACGTCTTCACCAACTGCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGCCCGCCGGCTGCCGTAGCTGATg                                                                                                                                                                                               <  2:408426/243‑1 (MQ=255)                                            ggtcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTctct                                                                                                                                                                                          <  1:83539/248‑1 (MQ=255)                                            ggtcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGAGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAACGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCTCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGTCGGCCGGAGCTGTTGActct                                                                                                                                                                                          >  2:69846/4‑251 (MQ=255)                                             ggcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCGGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCAGCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTctg                                                                                                                                                                                         <  1:325075/249‑1 (MQ=255)                                                           gTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTAGCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATgacg                                                                                                                                                                            <  2:35219/250‑1 (MQ=255)                                                              ggTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACAt                                                                                                                                                                        <  1:281425/251‑1 (MQ=255)                                                                     ttGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCGGCGCGATGCCGACCTCCTgctc                                                                                                                                                                 >  2:104009/1‑248 (MQ=255)                                                                         agACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTc                                                                                                                                                              >  1:417186/2‑250 (MQ=255)                                                                           aCATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCAct                                                                                                                                                           <  1:418641/251‑2 (MQ=255)                                                                             aTCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCActgc                                                                                                                                                         >  2:178070/1‑248 (MQ=255)                                                                                 aCGCCAACTGCGGTATCACCCAGAAAGTGAAAGCAACTGGCCTGGACGCGCTTTTTGACGCCACCATCAAAGCGATGAAAGTAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCATCGTCGCAGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCACTGCTGAGCGAGGACGACATCCTGATTCTCActgaggac                                                                                                                                                     >  2:9445/1‑244 (MQ=255)                                                                                  cGCCAAGTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACTATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACGCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGAcc                                                                                                                                                    <  2:353786/251‑1 (MQ=255)                                                                                    ccAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCAc                                                                                                                                                  <  1:273171/251‑1 (MQ=255)                                                                                       aCTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTGGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGt                                                                                                                                               <  1:335448/251‑1 (MQ=255)                                                                                        cTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGtt                                                                                                                                              >  1:258980/1‑251 (MQ=255)                                                                                             gtctCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGACTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGGCCACGGTTCTGCTGCGGGTCTGGCGCTGTTCGGCCGCCGTCTGCCGGAGCTGCTGTCTCTTCTGCGCGATTGCGCCATCCTGAACCTCAATGATGACCACGGTTGTGA‑t                                                                                                                                        >  2:342077/4‑251 (MQ=255)                                                                                               aTCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑Tc                                                                                                                                       <  2:129709/250‑1 (MQ=255)                                                                                                 cACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGtc                                                                                                                                                                                             <  2:448162/195‑1 (MQ=255)                                                                                                 cACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGtc                                                                                                                                                                                             >  1:448162/1‑195 (MQ=255)                                                                                                   ccAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGAGAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCGGGTCGACCGCCGTCTGCCGGAGCGGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGAcc                                                                                                                                  <  2:100455/251‑1 (MQ=255)                                                                                                          agtgaaagcacacggCCTGGACGAGCTGTTTTACGCAACCATCAAAGAGATGAAAGAAGCGGGTGAGAACACCATCGTCTTCACCAACTTCGTTGACGTCGACTCTTCCTGGGGCCACCGTCGCGTCGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCg                                                                                                                           <  2:15262/238‑1 (MQ=255)                                                                                                               aatcaaCTGGCCTGGACGCGCTGTTTGACGCCTCCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTTCCCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGTGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACt                                                                                                                      <  1:55017/246‑1 (MQ=255)                                                                                                                   aacgggCCGGGACTCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGTGTGATAACACCCTCGTCTTCACCATCGTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGAcc                                                                                                                  <  2:313040/247‑1 (MQ=255)                                                                                                                    aCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACca                                                                                                                 <  1:420027/251‑1 (MQ=255)                                                                                                                          cTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGCTCCTCAAAGCTGACCAAGGATG‑GAGTCCGACATGGACCGGCACTGGCCACAcgcg                                                                                                           >  2:105403/1‑251 (MQ=255)                                                                                                                                     gTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTc                                                                                                <  1:136298/251‑1 (MQ=255)                                                                                                                                        tgccGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACCCCATCGTCTGCCCCTACGTCGTTGACTTCGACTCTTCCTGTGGCCTCCGTCGCGACGTCGCCGTTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGGCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCg                                                                                              <  2:86987/247‑1 (MQ=255)                                                                                                                                             cccccctCAAATAGATGAAAGAAGCGGGGGATAACACCAGTGTCGTCACCAACCTCGTTGACATCGACTCTTCCGGGGGCTACCGTCGCGGCGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGTCCGGTACTGACCACACGCGTGAacagatcggaagatct                                                                                        <  2:136303/245‑14 (MQ=255)                                                                                                                                              caccaTCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGCTGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGC‑TCCGACCTGGACCGGTACTGCCCACACGCGTGAACACATTACGGTACTgg                                                                                       >  1:26583/1‑251 (MQ=255)                                                                                                                                                    cAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttac                                                                                  <  1:327556/250‑5 (MQ=255)                                                                                                                                                      aaGAGATGAAAGAAGCGGGTGATAACACCTTCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacggc                                                                               <  1:332951/251‑8 (MQ=255)                                                                                                                                                      aaGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTACCGGAGCTGATGTCTCTGCTGCGCGATGGAGACATCCTGATCCTCACTGCTGACACCGGTTGTGA‑TCCGACCTGGACCGGTCCTGACCACACGCGTGAACAAAGTCAGGTACTGGtttacggc                                                                               >  2:459572/1‑244 (MQ=255)                                                                                                                                                       agagATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGcc                                                                              >  1:436197/1‑251 (MQ=255)                                                                                                                                                               aagaagCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTa                                                                      <  2:417186/251‑1 (MQ=255)                                                                                                                                                                  aagCGGGTTATAACCCCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaaaa                                                                   <  1:321383/251‑1 (MQ=255)                                                                                                                                                                          gctAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACCTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGcac                                                           >  2:46224/3‑249 (MQ=255)                                                                                                                                                                                 ccATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGt                                                   <  1:312130/251‑1 (MQ=255)                                                                                                                                                                                   aTCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTcc                                                 >  2:79883/1‑250 (MQ=255)                                                                                                                                                                                     cGTCTTCACCAATGTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCacc                                               <  1:222263/251‑3 (MQ=255)                                                                                                                                                                                           cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGaa                                          <  1:346761/250‑1 (MQ=255)                                                                                                                                                                                           cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGAAGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGGCCACACGCGTGACCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGC‑CACCTGGGTCacagtgca                                          >  2:163195/1‑243 (MQ=255)                                                                                                                                                                                                cctgcGTTGGCTTCGACTCTGCCTGGGGCCACCGTCGCGGCGTCGCCGGTTAGGCTGCGGGTCTGGGGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTTCTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGTGTCACCGTGAAACCTTc                                    <  2:26583/247‑1 (MQ=255)                                                                                                                                                                                                    cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTcgcg                                 <  1:135919/250‑1 (MQ=255)                                                                                                                                                                                                    cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCgg                                <  1:242039/251‑1 (MQ=255)                                                                                                                                                                                                          cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGATCACGGTTGCGA‑TCCGACCTGGAACGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAACGTAAAACCGGGATCAC‑TGGTTCATCGTGAAACCTTCGCGGATATCg                          >  2:210759/1‑251 (MQ=255)                                                                                                                                                                                                             cGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGatattggt                        <  1:150484/250‑5 (MQ=255)                                                                                                                                                                                                                     ccTGGGGCCACCTCCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGTCCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGGTGGGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg               <  2:109635/251‑1 (MQ=255)                                                                                                                                                                                                                      cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAcgcttg              >  2:295715/1‑246 (MQ=255)                                                                                                                                                                                                                      cTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTgg              >  1:172214/1‑251 (MQ=255)                                                                                                                                                                                                                       tGGGGCCACAGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGCCGACATCCTGATCCACACTGCTGCCCACGTTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACTGCACGAAAGTAAAACAGGTCTCCC‑TGGCACACCGTGAAACCCTCGCGGatattggttagactcatgc             >  2:259204/1‑236 (MQ=255)                                                                                                                                                                                                                           gCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTa                                                                                           <  1:61594/170‑1 (MQ=255)                                                                                                                                                                                                                           gCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTa                                                                                           >  2:61594/1‑170 (MQ=255)                                                                                                                                                                                                                                 gTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACCTCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGCCCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACATTCGCGGATATTGGACAGCCTCTGGCAAAATAtttt   >  1:454689/1‑251 (MQ=255)                                                                                                                                                                                                                                  gcgcgACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTg  <  1:104009/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        GCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGA‑TCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCAC‑TGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTG  >  NC_000913/4620313‑4620788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑