Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,385	G→A	A261A (GCG→GCA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,385	0	G	A	98.3%	171.7 / ‑4.9	60	A261A (GCG→GCA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (30/28);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

CCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATC  >  NC_000913/4620138‑4620631                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cctgccACGAAGGAACCTTTGGCCTGGATAAACTCTTCGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGAc                                                                                                                                                                                                                                                     <  1:383607/251‑1 (MQ=255)      cacgaaggaacctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTa                                                                                                                                                                                                                                                <  1:451566/233‑1 (MQ=255)             gaacctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGGTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCCGCGTACCGGTAAACGTCACGACCTGGCTGTTGAGCCCCCCGCACCGACCGTGCTGCGGAAACTGGTTGATGAAAAACACGGTCCGGGGGTTTCTGTCGGTGACATTGCAGACATCTCCGCCAAc                                                                                                                                                                                                                                         >  2:150484/12‑251 (MQ=255)                       ccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGGCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCa                                                                                                                                                                                                                               <  1:471687/250‑1 (MQ=255)                         tGGATAAACTCTACGAACTGTGCGCAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCCCATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCATCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCCCCAGCACCCACCCTTCTGCCGAAACCGGTTTATGTAAAACCCGGTCCGGTGGTTTCTGTCGGTAAAATTGCAGACATTTCCCCCAACTGCGGTATcccc                                                                                                                                                                                                                             >  2:242039/1‑248 (MQ=255)                         tGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGGTGATGGAAAAAACGGTCAGGGGGTTTCTGTCGGTAAAATTGCAGACATCTACCCCCACTTCGGTATCAcc                                                                                                                                                                                                                             >  2:312130/1‑251 (MQ=255)                              aaaCTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGAACGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGGCGGTAAAATTGCAGACATCTACGCCAACTGCGGAATCAccca                                                                                                                                                                                                                           >  2:61316/1‑246 (MQ=255)                               aaCTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTTGTGGTATCACTCGTCCGTTTATCGGTTCCAAAAACGTTAACTTCCATCGTACCGGAAACCGTCACGACCTGGGTGTTCTGCCGCCCACACCGGCCGGGCTGCCGAAACCGGTTGATGCAAAAAACGGTCAGGGGGATTCTGTCGGTAGAATTGCAGACCTCTACGCCTACTGccggctgatcaagga                                                                                                                                                                                                                        >  1:240846/1‑236 (MQ=255)                                  tctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGGATCACCAAGAAAGTg                                                                                                                                                                                                                    >  1:111667/1‑251 (MQ=255)                                   ctACGAACTGGGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTACCGGTGACAAAGCCGGTAACTGCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGt                                                                                                                                                                                                                     <  1:298971/249‑1 (MQ=255)                                     ccGAACTGTGCGAAATCGCCCGTGAAGAGCTGTCCACCGGCGGCTACAACATCGGTCGTGTTATCGCTCTTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGaaa                                                                                                                                                                                                                 <  2:146232/250‑1 (MQ=255)                                      cGAACGGTGCGAAATCGCCCGTTAAGAGCTGCCCAACGGCGGCTACAACATCGTTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCTGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAg                                                                                                                                                                                                                <  1:280328/251‑1 (MQ=255)                                          cTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGGGTTATCGCTCGTCCGGTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGGCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGGAAAATTGCCAACATCTACGCCAACTGAGGTGTCCCCAAGAAAGTGaaatcaac                                                                                                                                                                                                            >  1:129709/1‑246 (MQ=255)                                             tgCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTgg                                                                                                                                                                                                         >  1:35219/1‑251 (MQ=255)                                              gctaaaTCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTTGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGc                                                                                                                                                                                                        <  1:203260/248‑1 (MQ=255)                                                gAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGCCCGGGCTGCAGCAACTGGTTGATGAAAAACCCGGTCCGGTGGTTTCTGTCGGTAAAATTGCAGACATCTTCCCCAACTGCGGGATCACCAAGAAAGTGAAAGCAACTGGCCt                                                                                                                                                                                                      >  1:385645/1‑251 (MQ=255)                                                      gCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGAcgc                                                                                                                                                                                                >  1:158733/1‑251 (MQ=255)                                                      gCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCCCCAGCACCGACCGTGCTGCAGAAAATGGTTGATGAAAAACACGGTCCGGGGGTTTCTGTCGGTAAAAATGCAGACATCTACCCCAACTGCGGTTTCACCCAGAAAGTGAAAGCAACTGGCCTGGaagc                                                                                                                                                                                                >  2:273171/1‑248 (MQ=255)                                                        ccGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACgcgc                                                                                                                                                                                              >  1:41073/1‑251 (MQ=255)                                                           tGAAGATCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGCCGGTAACTTCCAGCGTACCGGGAACCTTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGGAAAACACGGGCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCCAGACAGAGAAAGCCACTTGCCTGGGCGCCCTgg                                                                                                                                                                                           >  2:135919/1‑250 (MQ=255)                                                           tGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGGGTTATCCCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGCGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGt                                                                                                                                                                                           >  1:109635/1‑251 (MQ=255)                                                           ggAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGt                                                                                                                                                                                           <  2:158733/250‑1 (MQ=255)                                                            gAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGtt                                                                                                                                                                                          <  1:260691/251‑1 (MQ=255)                                                             aaGACCTGACCAACGGCGGCTACAACACCGGTCGTGTTATCGCTCGCCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTAGCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGttt                                                                                                                                                                                         <  2:385645/251‑1 (MQ=255)                                                                agCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGAc                                                                                                                                                                                      <  1:61316/251‑1 (MQ=255)                                                                  cTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGGAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGc                                                                                                                                                                                    >  1:253278/1‑251 (MQ=255)                                                                    gACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTTATGAAAAACAAGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCAACAAGAAAGTGAACGCAACTGGCCTGGACGCGCTGTTTGGCGccc                                                                                                                                                                                  >  1:267383/1‑250 (MQ=255)                                                                              ggcTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGCCAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCaa                                                                                                                                                                           >  1:186092/1‑248 (MQ=255)                                                                               gcTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCCGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCCCCGACCGCGCTGCAGCAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGCCCTCTACGCCAACTGCGGTATCACCACGAAAGTGAAAGCAACTGGCCTGGAGGCGCCGTTTGACGCCCCCATCAAaga                                                                                                                                                                        >  2:325075/1‑250 (MQ=255)                                                                                        tCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCAGTCGGTAAAATTGCAGACATCTACGCCAACTGCTGTATCACCAAGCAAGTGAACGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATAAAAGAGATGAaaga                                                                                                                                                               >  1:323095/1‑250 (MQ=255)                                                                                             cGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCggg                                                                                                                                                         <  1:46168/251‑1 (MQ=255)                                                                                                 ttatcgcactcccGTTTAGCGGTGACAAAGCCGGGAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTGGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATGGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTgt                                                                                                                                                      <  2:267383/239‑2 (MQ=255)                                                                                                       cccGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaagaaa                                                                                                                                                                                                                       <  2:222799/177‑1 (MQ=255)                                                                                                          gTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTGGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTTGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATc                                                                                                                                            <  1:113557/251‑1 (MQ=255)                                                                                                          gTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGGCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATc                                                                                                                                            <  2:253278/251‑1 (MQ=255)                                                                                                           cccGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCg                                                                                                                                           <  2:41073/250‑1 (MQ=255)                                                                                                                     ggtgACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCa                                                                                                                                  >  1:80542/4‑250 (MQ=255)                                                                                                                              gCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTACAATTGCAGACATCTACGCCAACTGCGGTAACACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCCCCATCCAAGAGATGACAGAAGCGGGTGAAAACACCATCGACTTCACCTACTTCGTTgc                                                                                                                        >  2:63424/1‑250 (MQ=255)                                                                                                                                   tAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGACAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTTGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCg                                                                                                                   >  2:281425/1‑251 (MQ=255)                                                                                                                                    aaCTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCg                                                                                                                   >  2:483203/1‑250 (MQ=255)                                                                                                                                        tCCATCGTACCGGTCACCGTTCCGACCTAGCTGGTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGGCACCATCAAAGAGATGAAAGAAGCGGGGGATAACGCCATCGTCTTCACCAACTTCGTTGACTTCGAcc                                                                                                                <  2:214355/249‑2 (MQ=255)                                                                                                                                         ccAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCtt                                                                                                             >  2:420027/1‑251 (MQ=255)                                                                                                                                           aGCGTACCGGGAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTcc                                                                                                           <  1:80353/251‑1 (MQ=255)                                                                                                                                                            aaCGACCTGGCTGTTGAGCCGCCAGCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCCCCTTcgcgc                                                                                          >  2:418095/2‑250 (MQ=255)                                                                                                                                                               gACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGCTGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGAATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTcgcgcagg                                                                                       >  2:346761/1‑247 (MQ=255)                                                                                                                                                                                gccAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCgg                                                                      >  1:328637/1‑251 (MQ=255)                                                                                                                                                                                   aGCACCGACCGTGCTGCAGAAACTTGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTc                                                                   <  1:51825/251‑1 (MQ=255)                                                                                                                                                                                       ccgaccgTGCTGAAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCTCCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGTTTATGCTTCGGGTCTGGa                                                               <  2:46873/251‑1 (MQ=255)                                                                                                                                                                                                  gcAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGAcc                                                    <  1:363452/251‑1 (MQ=255)                                                                                                                                                                                                          tGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTc                                                                                                                                      <  1:45748/161‑1 (MQ=255)                                                                                                                                                                                                          tGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTc                                                                                                                                      >  2:45748/1‑161 (MQ=255)                                                                                                                                                                                                           ggTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACCTCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGc                                           >  1:414099/1‑251 (MQ=255)                                                                                                                                                                                                                  gaAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCt                                    <  1:483203/251‑1 (MQ=255)                                                                                                                                                                                                                      aacacgttcAGGGGGTTTCTGTCGGTAAAATTGCAGGCATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATTAAAGAAGCGGGTGAGAACACCAACGTCTTCACCAACTGCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGCCCGCCGGCTGCCGTAGCTGATg                                <  2:408426/243‑1 (MQ=255)                                                                                                                                                                                                                           ggtcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTctct                           <  1:83539/248‑1 (MQ=255)                                                                                                                                                                                                                           ggtcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGAGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAACGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCTCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGTCGGCCGGAGCTGTTGActct                           >  2:69846/4‑251 (MQ=255)                                                                                                                                                                                                                            ggcAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCGGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCAGCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTctg                          <  1:325075/249‑1 (MQ=255)                                                                                                                                                                                                                                          gTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTAGCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATgacg             <  2:35219/250‑1 (MQ=255)                                                                                                                                                                                                                                             ggTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACAt         <  1:281425/251‑1 (MQ=255)                                                                                                                                                                                                                                                    ttGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCGGCGCGATGCCGACCTCCTgctc  >  2:104009/1‑248 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATC  >  NC_000913/4620138‑4620631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑